曲古霉素A预处理的hUC-MSCs移植对小鼠脑损伤的神经修复作用

发布时间：2018-07-01 14:19

本文选题：曲古霉素A + 人脐带间充质干细胞　；参考：《郑州大学学报(医学版)》2017年04期

【摘要】：目的:探讨30 nmol/L曲古霉素A(TSA)预处理的人脐带间充质干细胞(h UC-MSCs)移植对脑损伤(TBI)小鼠的神经修复和促进作用。方法:利用颅脑打击器和立体定位仪制作中度脑损伤小鼠模型,并将造模成功的27只小鼠随机分为Veh组、MSCs组和TSA+MSCs组;术后3天,PI染色评估各组小鼠脑损伤周围的细胞坏死情况,术后第1、2、3、7、14、21和28天,采用神经功能缺损程度评分(NDS评分)和糖水偏好实验评估小鼠神经运动感觉和抑郁情况;术后第28天采用qRT-PCR检测组蛋白去乙酰化酶1(HDAC1)和神经元特异性相关基因(DCX、MAP2)的表达。结果:与Veh组相比,MSCs组和TSA+MSCs组小鼠脑损伤周围的坏死细胞数明显减少,其中TSA+MSCs组降低更为明显(P0.001)。术后第1天,各组小鼠的NDS评分差异无统计学意义;从术后第3天开始,TSA+MSCs组小鼠NDS评分低于MSCs组和Veh组(P0.001);术后第28天,与其他两组相比,TSA+MSCs组小鼠蔗糖偏嗜度增强、HDAC1表达量降低,MAP2、DCX的表达量增加(P0.001)。结论:TSA预处理能够有效促进h UC-MSCs移植对TBI小鼠的神经修复。
[Abstract]:Aim: to investigate the effects of human umbilical cord mesenchymal stem cells (hUC-MSCs) pretreated with 30 nmol / L Tracocomycin A (TSA) on the neural repair and promotion of brain injury (TBI) mice. Methods: the mouse model of moderate brain injury was made by using craniocerebral punch and stereotactic locator, and 27 mice were randomly divided into two groups: Veh group and TSA MSCs group. On the 3rd day after operation, the cell necrosis around brain injury was evaluated by Pi staining. On the 21st and 28th days after operation, the neurological function deficit score (NDS) and the sugar water preference test were used to evaluate the neuromotor sensation and depression in the mice. The expression of histone deacetylase 1 (HDAC1) and neuron-specific associated gene (DCXfMAP2) were detected by qRT-PCR on the 28th day after operation. Results: compared with Veh group, the number of necrotic cells in TSA MSCs group and TSA MSCs group was significantly decreased, especially in TSA MSCs group (P0.001). The NDS scores in the TSA MSCs group were lower than those in the MSCs group and the Veh group from the 3rd day after operation (P0.001), and on the 28th day after operation, the NDS scores in the TSA MSCs group were lower than those in the MSCs group and the Veh group (P0.001). Compared with the other two groups, the expression of HDAC1 decreased in TSA MSCs group (P0.001). Conclusion the preconditioning of TSA can effectively promote the nerve repair of hUC-MSCs transplantation in TBI mice.
【作者单位】：郑州大学生命科学学院干细胞研究室;郑州大学第一附属医院骨科;
【基金】：国家自然科学基金资助项目U1404313,81471306和81601078 河南省高校科技创新团队15IRTSTHN022 河南省科技创新人才计划154200510008 河南省国际人才合作项目2016GH03,2016GH15
【分类号】：R651.15

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