富氢水对兔腰椎间盘退变的影响

发布时间：2018-07-12 19:37

本文选题：腰椎间盘退变 + 富氢水　；参考：《山东大学学报(医学版)》2017年03期

【摘要】：目的探讨腹腔注射富氢生理盐水对腰椎间盘退变的影响。方法 36只新西兰大白兔随机分为空白对照组、模型对照组和模型实验组,每组12只。各组均行右后外侧肌间隙入路显露腰椎间盘,空白对照组不做其他处理;模型对照组和模型实验组采用髓核穿刺抽吸法造腰椎间盘退变模型。模型实验组行腹腔注射富氢生理盐水8 mL/kg,每3天1次;模型对照组腹腔注射等量的等渗盐水;空白对照组不做任何处理。分别在4、8周行X线检查和组织病理学评价;采用ELISA法检测椎间盘组织中肿瘤坏死因子(TNF-α)、白细胞介素1(IL-1)、基质金属蛋白酶-3(MMP-3)含量。结果腰椎间盘退变模型用富氢生理盐水治疗4、8周后,在影像学和组织病理学方面均有明显改善;模型实验组椎间盘中的TNF-α、IL-1、MMP-3含量均明显低于模型对照组,高于空白对照组,差异有统计学意义(P0.001)。结论腹腔注射富氢生理盐水治疗兔腰椎间盘退变性疾病具有一定的积极作用。
[Abstract]:Objective to investigate the effect of intraperitoneal injection of hydrogen rich saline on the degeneration of lumbar intervertebral disc. Methods 36 New Zealand rabbits were randomly divided into blank control group, model control group and model experimental group, with 12 rats in each group. All groups were exposed to the lumbar intervertebral disc on the right posterior lateral muscle, and the blank control group did not do other treatment; model control group and model experiment Group a model of lumbar intervertebral disc degeneration was made by intraperitoneal injection of nucleus pulposus aspiration. The experimental group was injected intraperitoneally with hydrogen rich physiological saline 8 mL/kg, 1 times every 3 days. The model control group was injected with equal amount of isotonic saline, and the blank control group did not do any treatment. The X-ray examination and histological evaluation were performed at 4,8 weeks, and the ELISA method was used to detect the intervertebral disc tissue. The content of medium tumor necrosis factor (TNF- alpha), interleukin 1 (IL-1) and matrix metalloproteinase -3 (MMP-3). Results the lumbar disc degeneration model was significantly improved in imaging and histopathology after 4,8 weeks with hydrogen rich saline treatment. The content of TNF- alpha, IL-1, MMP-3 in the intervertebral disc of the model experimental group were significantly lower than that of the model control group. In the blank control group, the difference was statistically significant (P0.001). Conclusion intraperitoneal injection of hydrogen rich physiological saline in the treatment of lumbar intervertebral disc degeneration disease in rabbits has a positive effect.
【作者单位】：桂林医学院附属医院脊柱骨病外科;
【基金】：桂林市科技项目(20150206-1-8)
【分类号】：R681.53

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