右美托咪定对免疫低下幼鼠细胞免疫的影响

发布时间：2018-07-20 09:24

【摘要】：目的评价右美托咪定对脾切除幼鼠外周血T淋巴细胞增殖及T细胞亚群的影响。方法健康雄性SD大鼠24只,6周龄,体重130~150g,随机分为四组:生理盐水组(S组)、右美托咪定组(D组)、生理盐水组+脾切除(SN组)和右美托咪定组+脾切除(SD组),每组6只。S、D组不做外科处理,SN、SD组制备脾切除模型。喂养1周后S组和SN组腹腔注射生理盐水10ml/kg,D组和SD组腹腔注射右美托咪定50μg/kg。给药后2h,取血样,采用MTT法检测外周血T淋巴细胞增殖能力,采用流式细胞术检测T细胞亚群即CD4~+、CD8~+细胞含量,计算CD4~+/CD8~+值。结果与S组比较,SN组T淋巴细胞增殖能力降低,CD4~+、CD8~+含量及CD4~+/CD8~+值降低,D组T淋巴细胞增殖能力明显降低(P0.05);与D组比较,SD组T淋巴细胞增殖能力明显降低,CD4~+、CD8~+含量及CD4~+/CD8~+值明显降低(P0.05);与SN组比较,SD组T淋巴细胞增殖能力明显降低(P0.05)。结论右美托咪定对正常幼鼠和免疫低下幼鼠细胞免疫均有一定抑制作用,且对免疫低下幼鼠的抑制作用更强。
[Abstract]:Objective to evaluate the effect of dexmetomidine on T lymphocyte proliferation and T cell subsets in peripheral blood of splenectomy young rats. Methods 24 male Sprague-Dawley rats were aged 6 weeks. The rats were randomly divided into four groups: normal saline group (S group), dexmetomidine group (D group), splenectomy group (SN group) and desmetomidine group (SD group). One week after feeding, normal saline 10 ml / kg D and SD group were intraperitoneally injected with dexmetomidine 50 渭 g / kg / kg in S group and SN group. Two hours after administration, blood samples were collected and the proliferation ability of peripheral blood T lymphocytes was detected by MTT assay. The T cell subsets (CD4 ~ / CD8 ~ cells) were measured by flow cytometry, and the CD4 ~ / CD8~ value was calculated. Results compared with S group, the proliferation ability of T lymphocytes in S group decreased the content of CD4 ~ + CD8 ~ and CD4 ~ / CD8 ~ value of T lymphocyte in D group (P0.05), and the proliferation ability of T lymphocyte in SD group was significantly lower than that in D group (P0.05), and that in SD group was significantly lower than that in D group (P0.05). Compared with SN group, the proliferative ability of T lymphocytes in SD group was significantly lower than that in SN group (P0.05). Conclusion dexmetomidine has a certain inhibitory effect on the cellular immunity of both normal and immunodeficient young rats, and has a stronger inhibitory effect on the immunosuppression of young rats.
【作者单位】：兰州大学第一医院麻醉科;
【分类号】：R614

【参考文献】