腰椎间盘髓核组织中miR-200c、TrkB表达变化及其与退变程度的相关性

发布时间：2018-08-01 15:16

【摘要】：目的观察微小RNA-200c(miR-200c)与酪氨酸激酶受体B(Trk B)腰椎间盘髓核组织中的表达变化,并探讨其与腰椎间盘髓核退变程度的相关性。方法收集腰椎间盘髓核组织标本60例,用Pfirmlann分级对髓核退变程度进行分级,Ⅱ、Ⅲ、Ⅳ级各20例。采用细胞衰老β-半乳糖苷酶实验检测不同分级髓核组织中衰老髓核细胞百分比,RT-PCR技术检测髓核组织中miR-200c、Trk B mRNA表达,Western blotting技术检测髓核组织中Trk B蛋白表达,采用Pearson相关分析miR-200c、Trk B与衰老髓核细胞的关系。结果 PfirmlannⅡ、Ⅲ、Ⅳ级髓核组织中衰老髓核细胞百分比、miR-200c相对表达量、Trk B mRNA及蛋白相对表达量两两比较,P均0.05。miR-200c mRNA与Trk B mRNA相对表达量呈负相关(r=-0.71,P0.05),miR-200c mRNA相对表达量与衰老髓核细胞百分比呈正相关(r=0.75,P0.05),Trk B mRNA相对表达量与衰老髓核细胞百分比呈负相关(r=-0.70,P0.05)。结论腰椎间盘髓核退变程度越高,miR-200c表达越高,Trk B mRNA表达越低;miR-200c可能通过下调Trk B表达参与腰椎间盘髓核组织的退变过程。
[Abstract]:Objective to observe the expression of small RNA-200c (miR-200c) and tyrosine kinase receptor (B (Trk B) in nucleus pulposus of lumbar disc and to explore the relationship between miR-200c and degeneration of nucleus pulposus of lumbar intervertebral disc. Methods 60 specimens of nucleus pulposus of lumbar intervertebral disc were collected. The grade of degeneration of nucleus pulposus was graded by Pfirmlann classification, including 20 cases of grade 鈪，

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