异丙酚抑制高糖诱导的脐静脉内皮细胞黏附分子表达

发布时间：2018-08-03 08:55

【摘要】：目的研究异丙酚抑制高糖诱导的脐静脉内皮细胞黏附分子的表达并探讨其可能的机制。方法采用Histopaque-1077溶液提取人外周血单核细胞。采用一氧化氮(NO)试剂盒检测脐静脉内皮细胞NO生成。采用Western blot检测内皮细胞黏附分子、内皮型一氧化氮合酶(endothelial nitric oxide synthase,e NOS)(总蛋白,单体及双体)、e NOS磷酸化水平及caveolin-1表达。结果高糖上调血管内皮细胞黏附分子1(vascular cell adhesion molecule,VCAM-1)的表达,促进单核细胞-内皮黏附,并减少NO生成。异丙酚改善高糖环境下NO生成,并抑制VCAM-1表达及单核细胞-内皮黏附。异丙酚的作用可被e NOS抑制剂L-NAME所拮抗。异丙酚能上调高糖环境下e NOS-Ser1177磷酸化水平及双体/单体比值,下调高糖环境下e NOS-Thr495磷酸化水平及caveolin-1表达。结论异丙酚通过调节高糖环境下e NOS的磷酸化水平、单体/双体比值及caveolin-1表达,改善内皮细胞NO生成,进而抑制内皮细胞黏附分子的表达及单核细胞-内皮细胞的黏附。
[Abstract]:Aim to investigate the inhibitory effect of propofol on the expression of high glucose induced adhesion molecules in umbilical vein endothelial cells (HUVECs) and its possible mechanism. Methods Human peripheral blood monocytes were extracted with Histopaque-1077 solution. Nitric oxide (no) production in umbilical vein endothelial cells (HUVEC) was detected by nitric oxide (NO) kit. Western blot was used to detect the phosphorylation of NOS and the expression of caveolin-1 in endothelial cell adhesion molecule, (endothelial nitric oxide synthasee NOS) (total protein, monomer and double body. Results High glucose upregulated the expression of vascular endothelial cell adhesion molecule 1 (vascular cell adhesion molecule-1 (VCAM-1), promoted monocyte-endothelial adhesion and reduced no production. Propofol improved no production and inhibited VCAM-1 expression and monocyte-endothelial adhesion. The effect of propofol was antagonized by e NOS inhibitor L-NAME. Propofol could up-regulate the level of e NOS-Ser1177 phosphorylation and the ratio of double body to monomer, and down-regulate the level of e NOS-Thr495 phosphorylation and the expression of caveolin-1 in high glucose environment. Conclusion propofol can improve the production of no in endothelial cells by regulating the phosphorylation level of e NOS, monomeric / catamarone ratio and caveolin-1 expression in high glucose environment, and then inhibit the expression of adhesion molecules and monocyte / endothelial cell adhesion in endothelial cells.
【作者单位】：复旦大学附属肿瘤医院麻醉科;
【分类号】：R614

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