人参皂苷Rb1治疗兔耳增生性瘢痕相关机制的探索
发布时间：2018-08-03 11:54
【摘要】：目的:通过兔耳创建增生性疤痕模型,疤痕内注射药物人参皂苷Rb1,观察疗效及探索相关机理。方法:随机取8只大耳型新西兰白兔,每耳6处直径1cm圆形伤口,创建增生性疤痕模型,待其上皮化后设空白组(N),地塞米松阳性对照组(D),生理盐水对照组(S),人参皂苷Rb1组(R)。其中地塞米松组及人参皂苷组均应用生理盐水配置浓度为1mg/ml,疤痕内注射药物,每次0.3ml,每三天1次,共三次。注射后第1、2、4、8周随机收集2只兔子标本(24例)行HE染色观察瘢痕增生指数(SEI)、行Masson染色观察胶原增生情况、免疫组化法检测增值细胞核抗原(PCNA)表达、逆转录聚合酶链反应检测转化生长因子β(TGF-β)及Ⅰ型胶原表达。各组数据除以空白组数据进行数据标准化。软件使用SPSS19.0,计量资料以“x±s”表示,使用方差分析,若数据不符合正态性及方差齐性,改用非参数检验。选α=0.05,当P≤0.05,数据有统计学意义。结果:用药后第1、2周三组间未见统计学差异,第4、8周,D及R组较S组瘢痕增生改善明显,并随时间的延长疗效增加,SHI降低(P0.05),胶原增生面积减少(P0.05),PCNA表达降低(P0.05),TGF-β1及Ⅰ型胶原m RNA表达降低(P0.05),但D组及R组间未见统计学差异。结论:局部注射人参皂苷Rb1能抑制活性细胞增殖,血管生成减少,抑制TGF-β1表达,胶原合成减少,有效改善兔耳疤痕的增生,为使用人参皂苷Rb1防治疤痕形成提供理论基础。
[Abstract]:Aim: to establish a hypertrophic scar model in rabbit ear and inject ginsenoside Rb1 into the scar to observe the curative effect and explore the related mechanism. Methods: eight New Zealand white rabbits with large ears were randomly selected, with 6 diameter 1cm round wounds in each ear, and then the hypertrophic scar model was created. After epithelization, a blank group of (N), dexamethasone positive control group, (D), normal saline control group, (S), ginsenoside Rb1 group (R). Was set up. The dexamethasone group and ginsenoside group were treated with saline (1 mg / ml). The drug was injected into the scar with 0.3 ml once every three days for three times. Two rabbits (24 cases) were randomly collected at 8 weeks after injection to observe the proliferation of collagen by Masson staining and the expression of proliferating cell nuclear antigen (PCNA) by immunohistochemical staining. The expression of transforming growth factor 尾 (TGF- 尾) and type I collagen were detected by reverse transcriptase polymerase chain reaction (RT PCR). Each group of data divided by blank group data for data standardization. The software uses SPSS 19.0, the measurement data is expressed as "x 卤s", and the analysis of variance is used. If the data do not conform to normality and homogeneity of variance, the non-parametric test is used. When P 鈮，
本文编号：2161662
[Abstract]:Aim: to establish a hypertrophic scar model in rabbit ear and inject ginsenoside Rb1 into the scar to observe the curative effect and explore the related mechanism. Methods: eight New Zealand white rabbits with large ears were randomly selected, with 6 diameter 1cm round wounds in each ear, and then the hypertrophic scar model was created. After epithelization, a blank group of (N), dexamethasone positive control group, (D), normal saline control group, (S), ginsenoside Rb1 group (R). Was set up. The dexamethasone group and ginsenoside group were treated with saline (1 mg / ml). The drug was injected into the scar with 0.3 ml once every three days for three times. Two rabbits (24 cases) were randomly collected at 8 weeks after injection to observe the proliferation of collagen by Masson staining and the expression of proliferating cell nuclear antigen (PCNA) by immunohistochemical staining. The expression of transforming growth factor 尾 (TGF- 尾) and type I collagen were detected by reverse transcriptase polymerase chain reaction (RT PCR). Each group of data divided by blank group data for data standardization. The software uses SPSS 19.0, the measurement data is expressed as "x 卤s", and the analysis of variance is used. If the data do not conform to normality and homogeneity of variance, the non-parametric test is used. When P 鈮，
本文编号：2161662
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