TRPM4通道抑制剂9-Phenanthrol在模拟心内直视术下对幼犬心肌的保护作用

发布时间：2018-08-04 22:04

【摘要】：目的:观察瞬时受体电位M4型(transient receptor potential melastatin 4,TRPM4)通道抑制剂9-Phenanthrol在模拟心内直视下对幼犬心肌的保护作用。方法:选取18只种类相同,年龄小于8周,体重相近且健康的幼犬作为研究对象,随机分为9-Phenanthrol组(n=9)和对照组(n=9)。对照组在体外循环建立前20分钟给予生理盐水,9-Phenanthrol组体外循环建立前20分钟给予TRPM4通道抑制剂9-Phenanthrol。然后,应用ELISA测定并分别对比两组幼犬在主动脉阻断后30分钟(T1)及60分钟(T2)、主动脉开放后1小时(T3)及2小时(T4)时刻的血清肌酸激酶同工酶MB(creatine kinase isoenzyme MB,CK-MB)以及肌钙蛋白I(cardiac troponin I,cTn-I)水平的变化,并应用免疫组化和HE染色分析TRPM4在各时刻相同部位心肌组织的表达情况及心肌组织变性程度。结果:于T1、T2、T3、T4时刻,对照组和9-Phenanthrol组幼犬血清CK-MB及cTn-I呈升高趋势,而9-Phenanthrol组血清各指标明显低于对照组,差异均具有统计学意义(P0.05)。与对照组相比,9-Phenanthrol组幼犬心肌组织破坏较轻;9-Phenanthrol组幼犬心肌组织中TRPM4蛋白的表达比对照组中表达有所减弱。结论:TRPM4通道抑制剂9-Phenanthrol在模拟心内直视术中的应用可对缺血再灌注导致的心肌损伤起到一定程度的保护作用,对未来应用于临床实验具有一定的价值。
[Abstract]:Aim: to observe the protective effect of 9-Phenanthrol, an inhibitor of transient receptor potential type M _ 4 (transient receptor potential melastatin _ 4 / TRPM _ 4, on myocardium of puppies under simulated intracardiac direct vision. Methods: eighteen puppies of the same species, age less than 8 weeks and similar and healthy puppies were randomly divided into two groups: 9-Phenanthrol group (n = 9) and control group (n = 9). The control group was given TRPM4 channel inhibitor 9-Phenanthroll 20 minutes before CPB. And then. The changes of serum creatine kinase isoenzyme (MB (creatine kinase isoenzyme MBK-MB) and troponin (I (cardiac troponin ItcTn-I) were measured and compared by ELISA at 30 minutes (T1) and 60 minutes (T2) after aortic occlusion, 1 hour (T3) and 2 hours (T4) after aorta opening. Immunohistochemical staining and HE staining were used to analyze the expression of TRPM4 in myocardium at the same time and the degree of myocardial degeneration. Results: at the time of T1T2T3T4, the serum CK-MB and cTn-I of puppies in control group and 9-Phenanthrol group were increased, while the serum indexes in 9-Phenanthrol group were significantly lower than those in control group (P0.05). Compared with the control group, the expression of TRPM4 protein in the myocardial tissue of 9-Phenanthrol group was lower than that in the control group. Conclusion the application of 9-Phenanthrol in simulated open heart surgery can protect myocardial injury induced by ischemia and reperfusion to a certain extent, and has certain value for clinical trials in the future.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R654.2

【参考文献】