七氟烷与高浓度氧对老年小鼠海马caspase-3的表达及认知功能的影响

发布时间：2018-08-12 10:08

【摘要】：目的探讨七氟烷与高浓度氧对老年小鼠海马半胱氨酸蛋白酶-3(caspase-3)的表达及认知功能的影响。方法健康雄性C57/BL6老年小鼠(14月龄)33只,采用数字表法将其随机分为3组:高浓度氧组(HO组,n=11):吸100%(体积分数)氧4h;高浓度氧+七氟烷组(HS组,n=11):吸100%(体积分数)氧+2.5%(体积分数)七氟烷4 h;对照组(Con组,n=11):吸空气4 h。24 h后采用Morris水迷宫法研究3组小鼠麻醉后连续5 d找到平台所用的时间(逃避潜伏期)和第5天去除平台后,90 s内穿过原平台的次数。利用蛋白质印记(Western blotting)法检测小鼠海马caspase-3的表达。结果与Con组相比,HO组的caspase-3表达差异无统计学意义(P0.05);HS组的穿越平台次数降低(P=0.023),caspase-3表达显著增加(P=0.036)。结论吸入100%(体积分数)氧对老年小鼠认知功能无影响,2.5%(体积分数)七氟烷与100%(体积分数)氧同时吸入增加老年小鼠海马caspase-3的表达,与老年小鼠产生认知功能障碍有关。
[Abstract]:Objective to investigate the effects of sevoflurane and high concentration oxygen on the expression of cysteine protease-3 (caspase-3) and cognitive function in hippocampus of aged mice. Methods 33 healthy male C57/BL6 mice (14 months old) were used. They were randomly divided into three groups by digital table: high concentration oxygen group (Ho group): 100% oxygen (volume fraction) for 4 hours; high concentration oxygen sevoflurane group (HS group): inhalation of 100% oxygen (volume fraction) for 4 hours; control group (Con group n 11): The Morris water maze method was used to study the time (escape latency) of finding the platform after anesthesia for 5 days and the times of crossing the original platform within 90 seconds after the removal of the platform on the 5th day after inhaling air for 4 h. 24 h. Protein imprinted (Western blotting) method was used to detect the expression of caspase-3 in hippocampus of mice. Results compared with Con group, there was no significant difference in the expression of caspase-3 between the two groups (P0.05). In HS group, the frequency of crossing platform decreased (P0. 023) and the expression of caspase-3 increased significantly (P0. 036). Conclusion inhalation of 100% (volume fraction) oxygen has no effect on cognitive function of aged mice. Both 2.5% (volume fraction) of sevoflurane and 100% (volume fraction) of oxygen simultaneously increase the expression of caspase-3 in hippocampus of aged mice, which is related to cognitive impairment in aged mice.
【作者单位】：首都医科大学附属北京胸科医院麻醉科;国家癌症中心/中国医学科学院北京协和医学院肿瘤医院麻醉科;
【基金】：国家自然科学基金(81371202)~~
【分类号】：R614

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