去骨瓣减压对大鼠心肺复苏后大脑中动脉血流、S100b及NSE蛋白表达的影响
发布时间：2018-08-13 08:33
【摘要】：目的研究去骨瓣减压术对窒息性心脏骤停大鼠复苏脑神经元特异性烯醇化酶(NSE)、S-100b蛋白(S-100)表达及脑血流量的影响,探讨去骨瓣减压对窒息性心跳骤停大鼠复苏脑所产生的保护作用以及产生这种保护作用的可能机制,为窒息性心跳骤停的临床治疗提供理论依据。方法1实验动物及分组成年清洁雄性Wistar大鼠48只,体重250~370 g,随机分为假手术组、复苏组、去骨瓣组,再分别按气管插管+股动静脉置管术后(假手术组)或自主循环恢复(ROSC)后(复苏组、去骨瓣组)分为6 h亚组,12 h亚组,24 h亚组,48 h亚组这4个亚组,每个亚组4只。实验之前大鼠禁食至少12 h,但不禁水。2实验模型制作采用改良型的窒息法制备大鼠心脏骤停复苏模型。复苏组与去骨瓣干预组采用窒息致大鼠心脏骤停(CA)和心肺复苏(CPR)模型。去骨瓣干预组在大鼠心肺复苏术成功后1h施行去骨瓣减压术。假手术组仅行气管插管、股动静脉置管术。3去骨瓣干预治疗去骨瓣各亚组(6 h,12 h,24 h,48 h)大鼠于窒息性心跳骤停复苏模型成功1小时后行去骨瓣干预。4术后大脑中动脉血流的监测各亚组大鼠分别按照时间点,于6h、12h、24h及48h后,使用激光多普勒血流仪(LDF)监测大鼠心肺复苏前后大脑中动脉血流的变化。5标本的采集在不同的时间点取大鼠静脉血,离心留取血清,采用Elisa方法,检测血清中NSE和S100b蛋白的表达,然后断头取脑,行HE染色,分析各实验指标与去骨瓣减压术的相关性。6统计学方法文中数据均以(均数±标准差)表示,应用SPSS 17.0统计学软件对本实验数据进行分析,组间比较采用单因素方差分析,组内比较采用卡方检验。结果以P0.05为差异具有统计学意义。结果1假手术组各亚组大鼠气管插管及动静脉置管前后大脑中动脉血流无明显异常变化。与假手术组比较,复苏组、去骨瓣干预组大脑中动脉血流在自主循环恢复(ROSC)后6h降低,而且会持续降低至48h,实验结果的差异具有统计学上的意义(P0.05);与复苏组比较,去骨瓣干预组大脑中动脉血流在自主循环恢复(ROSC)后6h至48h降低幅度较小,6h、12h、24h、48h的实验结果差异有显著性(P0.05)。2血清中NSE蛋白的浓度:与假手术组比较,复苏组及去骨瓣减压组血清NSE蛋白在自主循环恢复(ROSC)后6h明显升高,而且会持续上升至12 h达到峰值,在24 h和48h有所回落,差异具有统计学意义(P0.01);去骨瓣减压组与复苏组比较,去骨瓣减压组ROSC后6 h血清内NSE蛋白浓度较复苏组无明显差异,没有统计学上的意义(P0.05)然而实验12 h、24 h和48h后血清中NSE蛋白的浓度较复苏组明显降低,而且差异具有高度的显著性(P0.01)。3血清中S100b蛋白的浓度:与假手术组比较,复苏组及去骨瓣减压组血清S100b蛋白在自主循环恢复(ROSC)后6h明显升高,而且会持续上升到12 h达峰值,在24 h、48h时有所回落,差异高度显著,具有统计学意义(P0.01);去骨瓣减压组与复苏组比较,去骨瓣减压组在ROSC后6 h、12 h、24 h、48h时血清中S100b蛋白的浓度明显较复苏组减低,而且差异具有高度的显著性(P0.01)。结论去骨瓣减压能改善心肺复苏后大脑中动脉血流速度,并且可以使大鼠心肺复苏术后血清中NSE、S100b蛋白的表达降低,从而减轻颅脑的损伤。
[Abstract]:Objective To study the effect of decompression of bone flap on the expression of neuron-specific enolase (NSE), S-100b protein (S-100) and cerebral blood flow in the resuscitated brain of rats with asphyxiated cardiac arrest, and to explore the protective effect of decompression of bone flap on the resuscitated brain of rats with asphyxiated cardiac arrest and the possible mechanism. Methods 1 Experimental animals and 48 clean male Wistar rats weighing 250-370 g were randomly divided into sham-operation group, resuscitation group and bone flap removal group, and then divided into 6 groups according to tracheal intubation plus femoral artery and vein catheterization (sham-operation group) or autonomic circulation recovery (ROSC) respectively. Before the experiment, rats were fasting for at least 12 hours, but water was not forbidden. 2 The model of cardiac arrest resuscitation was made by modified asphyxia. The resuscitation group and the debridement intervention group were made by asphyxia-induced cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) models. The sham operation group was treated with tracheal intubation and femoral artery and vein catheterization. 3 The bone flap intervention was performed in each subgroup (6 h, 12 h, 24 h, 48 h). The changes of middle cerebral artery blood flow were monitored by laser Doppler flowmeter (LDF) at 6 h, 12 h, 24 h and 48 h after CPR in each subgroup. HE staining was used to analyze the correlation between the experimental indexes and decompression. 6 Statistical methods The data in this paper were expressed as mean (+ standard deviation). SPSS 17.0 statistical software was used to analyze the experimental data. One-way ANOVA was used to compare the data between groups. Chi-square test was used to compare the results between groups. Results 1 There were no significant changes in the middle cerebral artery blood flow before and after tracheal intubation and arteriovenous catheterization in the sham operation group. Compared with the sham operation group, the middle cerebral artery blood flow in the resuscitation group and the debridement intervention group decreased 6 hours after the recovery of spontaneous circulation (ROSC) and continued to decrease to 48 hours after the recovery of ROSC. Compared with the resuscitation group, the blood flow of middle cerebral artery in the debridement group decreased slightly from 6 hours to 48 hours after the recovery of spontaneous circulation (ROSC), and the experimental results showed significant difference (P 0.05). 2 Serum NSE protein concentration in the debridement group and the resuscitation group was significantly lower than that in the sham operation group (P 0.05). Compared with the resuscitation group, there was no significant difference in serum NSE protein concentration between the decompression group and the resuscitation group (P 0.05). However, the serum levels of NSE protein were significantly lower at 12, 24 and 48 hours than those in the resuscitation group (P 0.01). 3 Serum levels of S100b protein were significantly different (P 0.01). Compared with the resuscitation group, the serum concentration of S100b protein in the decompression group was significantly lower than that in the resuscitation group at 6 h, 12 h, 24 h and 48 h after ROSC, and the difference was highly significant (P 0.01). The blood flow velocity of the middle cerebral artery after cardiopulmonary resuscitation decreased the expression of NSE and S100b proteins in the serum of rats after cardiopulmonary resuscitation, thus reducing the brain injury.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R651.1
本文编号：2180439
[Abstract]:Objective To study the effect of decompression of bone flap on the expression of neuron-specific enolase (NSE), S-100b protein (S-100) and cerebral blood flow in the resuscitated brain of rats with asphyxiated cardiac arrest, and to explore the protective effect of decompression of bone flap on the resuscitated brain of rats with asphyxiated cardiac arrest and the possible mechanism. Methods 1 Experimental animals and 48 clean male Wistar rats weighing 250-370 g were randomly divided into sham-operation group, resuscitation group and bone flap removal group, and then divided into 6 groups according to tracheal intubation plus femoral artery and vein catheterization (sham-operation group) or autonomic circulation recovery (ROSC) respectively. Before the experiment, rats were fasting for at least 12 hours, but water was not forbidden. 2 The model of cardiac arrest resuscitation was made by modified asphyxia. The resuscitation group and the debridement intervention group were made by asphyxia-induced cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) models. The sham operation group was treated with tracheal intubation and femoral artery and vein catheterization. 3 The bone flap intervention was performed in each subgroup (6 h, 12 h, 24 h, 48 h). The changes of middle cerebral artery blood flow were monitored by laser Doppler flowmeter (LDF) at 6 h, 12 h, 24 h and 48 h after CPR in each subgroup. HE staining was used to analyze the correlation between the experimental indexes and decompression. 6 Statistical methods The data in this paper were expressed as mean (+ standard deviation). SPSS 17.0 statistical software was used to analyze the experimental data. One-way ANOVA was used to compare the data between groups. Chi-square test was used to compare the results between groups. Results 1 There were no significant changes in the middle cerebral artery blood flow before and after tracheal intubation and arteriovenous catheterization in the sham operation group. Compared with the sham operation group, the middle cerebral artery blood flow in the resuscitation group and the debridement intervention group decreased 6 hours after the recovery of spontaneous circulation (ROSC) and continued to decrease to 48 hours after the recovery of ROSC. Compared with the resuscitation group, the blood flow of middle cerebral artery in the debridement group decreased slightly from 6 hours to 48 hours after the recovery of spontaneous circulation (ROSC), and the experimental results showed significant difference (P 0.05). 2 Serum NSE protein concentration in the debridement group and the resuscitation group was significantly lower than that in the sham operation group (P 0.05). Compared with the resuscitation group, there was no significant difference in serum NSE protein concentration between the decompression group and the resuscitation group (P 0.05). However, the serum levels of NSE protein were significantly lower at 12, 24 and 48 hours than those in the resuscitation group (P 0.01). 3 Serum levels of S100b protein were significantly different (P 0.01). Compared with the resuscitation group, the serum concentration of S100b protein in the decompression group was significantly lower than that in the resuscitation group at 6 h, 12 h, 24 h and 48 h after ROSC, and the difference was highly significant (P 0.01). The blood flow velocity of the middle cerebral artery after cardiopulmonary resuscitation decreased the expression of NSE and S100b proteins in the serum of rats after cardiopulmonary resuscitation, thus reducing the brain injury.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R651.1
【参考文献】
相关期刊论文 前4条
1 尚游,姚尚龙,袁世荧;心肺脑复苏的动物模型[J];国外医学.麻醉学与复苏分册;2004年03期
2 陈寿权,李章平,王姗姗,黄唯佳,程俊彦,王万铁,王为;窒息法致大鼠心脏骤停模型复苏的影响因素[J];中华急诊医学杂志;2005年10期
3 王效德;郭素香;;NSE、S100B蛋白在CNS损伤中的研究进展[J];中国现代医药杂志;2011年09期
4 王冰;胡世颉;李兵;高大宽;胡学安;刘伟;曹宝萍;费舟;;经颅多普勒对原发性脑出血病人脑血流的监测[J];中国微侵袭神经外科杂志;2015年02期
相关会议论文 前1条
1 贾楠;康熙雄;;卒中相关指标实验室研究现状及进展[A];第五次全国免疫诊断暨疫苗学术研讨会论文汇编[C];2011年
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