内植多元磁棒联合脉冲电磁场对兔骨及软骨损伤修复的实验研究
发布时间：2018-08-14 09:04
【摘要】：目的为探索研究磁场技术可行的应用加载方式,本研究以静磁体为原件构建用于体内植入的静磁体棒,明确体内引入的内置磁源联合体外脉冲电磁场在骨及软骨组织修复过程中的作用。方法1.根据前期在磁场方面的研究基础和相关文献报道,在生物工程领域专家的指导下,本研究选取磁体性能稳定的低磁感应强度钕铁硼永磁体材料作为基本组成原件,用于构建体内植入多元磁棒的内部磁极,磁极外部采用具有良好生物相容性和生物力学强度的医用钛合金材料进行密封包被,完成内植多元磁棒的物理属性测定;2.内植多元磁棒联合脉冲电磁场对兔骨缺损修复的实验研究:选取所有实验新西兰大白兔建立股骨髁骨缺损模型,依据实验干预方式分为联合磁场组、脉冲电磁场组和对照组,联合磁场组将内植多元磁棒植入兔股骨干远端髓腔内,术后体外联合脉冲电磁场(1h/d)进行试验干预,脉冲电磁场组和对照组分别在股骨干远端髓腔内植入无磁钛合金棒,同时,脉冲电磁场组术后在体外施加脉冲电磁场(1h/d),各组于术后第5周取股骨远端样本进行大体、组织学及影像学等方面评估;3.内植多元磁棒联合脉冲电磁场对兔软骨缺损修复的实验研究:选取所有实验新西兰大白兔建立股骨滑车软骨缺损模型,同样分为联合磁场组、脉冲电磁场组和对照组,联合磁场组将内植多元磁棒植入兔股骨干远端髓腔内,术后体外联合脉冲电磁场(1h/d)进行试验干预,脉冲电磁场组和对照组分别在股骨干远端髓腔内植入无磁钛合金棒,同时,脉冲电磁场组术后在体外施加脉冲电磁场(1h/d),各组分别于术后第4周和第10周取材进行大体观察、组织学及软骨基质成分等方面检测。结果1.本研究中研制的用于兔股骨干远端髓腔内植入的多元磁棒整体呈长度25.0mm,直径7.0 mm的类圆柱体,磁棒内部由钕铁硼永磁体和聚乙烯垫片相间排列组成,构成磁棒内部多元磁极,外部采用医用钛合金材料(Ti6Al4V)密封包被,磁棒表面磁场分布呈强弱相间变化,表面最大磁感应强度为40±5 m T;2.内植多元磁棒联合脉冲电磁场对兔骨缺损修复的实验研究:各组于术后第5周取材,显微CT扫描重建及组织病理学结果显示,联合磁场组外侧面骨质基本愈合完整,与周边骨质相延续,与脉冲电磁场组及对照组相比,皮质及皮质下区域新生骨质明显增多,骨小梁形态及连续性较好,显微CT骨组织定量分析结果显示,联合磁场组与对照组相比,骨缺损部位骨体积分数及骨小梁数量明显增加(P0.05),而骨小梁分离度下降(P0.01),骨小梁厚度无明显统计学差异,而与脉冲电磁场组相比,联合磁场组骨小梁数量增加(P0.05),骨小梁分离度下降(P0.01);3.内植多元磁棒联合脉冲电磁场对兔软骨缺损修复的实验研究:大体形态方面,术后第4周,联合磁场组软骨组织修复优于对照组(P0.05),与脉冲电磁场组无明显差别(P0.05),术后第10周,联合磁场组软骨组织修复大体形态评分显著优于对照组(P0.01)和脉冲电磁场组(P0.05),组织形态方面,术后第4周,联合磁场组组织形态评分明显优于对照组(P0.05),与脉冲电磁场组差异无统计学意义(P0.05),术后第10周,联合磁场组形组织形态评分显著优于对照组(P0.01),与脉冲电磁场组差异无统计学意义(P0.05),氨基聚糖(GAG)定量比较结果显示,术后第4周,对照组、脉冲电磁场组和联合磁场组中所测得的GAG含量分别为:12.26±4.43μg、12.48±3.91μg和13.02±2.83μg,各组间没有统计学差异;术后第10周,对照组、脉冲电磁场组和联合磁场组中所测得的GAG含量分别为:15.54±3.66μg、20.45±2.9μg和19.84±2.21μg,脉冲电磁场组和联合磁场组GAG含量明显高于对照组(P0.05),联合磁场组和脉冲电磁场组两者间差异无统计学意义(P0.05)。结论本研究以永磁体为基本组成原件构建的低磁感应强度可植入多元磁棒作为体内磁源,与体外脉冲电磁场共同构成联合磁场,联合磁场作用方式在相同组织创伤条件下相对单一脉冲电磁场及对照组具有更好的促进骨质形成,改善骨显微结构的作用,同时能够增强关节软骨的早期形态修复和界面整合,提高软骨组织中氨基聚糖基质成分的合成,本研究结果初步证实了内植磁场作用方式的可行性,对于局部靶向磁场治疗以及远期骨科植入材料的附磁应用等方面具有重要研究价值。
[Abstract]:Objective In order to explore a feasible loading method of magnetic field technology, magnetostatic rods for in vivo implantation were constructed with magnetostat as the original material, and the role of in vivo magnetic source combined with in vitro pulsed electromagnetic field in bone and cartilage tissue repair was clarified. Methods 1. According to the previous research foundation and related papers on magnetic field. Under the guidance of experts in the field of bioengineering, Nd-Fe-B permanent magnets with stable magnetic properties were selected as the basic components to construct the internal magnetic poles of multicomponent magnetic rods implanted in vivo. The external magnetic poles were made of medical titanium alloy materials with good biocompatibility and biomechanical strength. The physical properties of the implanted multivariate magnetic rods were measured by sealed coating. 2. The experimental study of implanted multivariate magnetic rods combined with pulsed electromagnetic field for repairing bone defects in rabbits: All experimental New Zealand white rabbits were selected to establish the femoral condyle defect model. According to the experimental intervention methods, they were divided into combined magnetic field group, pulsed electromagnetic field group and control group, combined magnetic field group. Pulsed electromagnetic field group and control group were implanted non-magnetic titanium alloy rods in the distal femoral shaft medullary cavity of rabbits respectively. Pulsed electromagnetic field group was implanted non-magnetic titanium alloy rods in the distal femoral shaft medullary cavity of rabbits after operation. Pulsed electromagnetic field group was applied in vitro after operation (1h/d). The femurs of each group were harvested at the 5th week after operation. The distal bone samples were grossly, histologically and radiologically assessed; 3. Experimental study of cartilage defect repair with implanted multivariate magnetic rods combined with pulsed electromagnetic field in rabbits: All experimental New Zealand white rabbits were selected to establish the femoral trochlear cartilage defect model, which was also divided into combined magnetic field group, pulsed electromagnetic field group and control group. Pulsed electromagnetic field group and control group were implanted non-magnetic titanium alloy rods in the distal femoral shaft medullary cavity of rabbits. Pulsed electromagnetic field group and control group were implanted non-magnetic titanium alloy rods in the distal femoral shaft medullary cavity of rabbits. Pulsed electromagnetic field group were applied in vitro after operation (1h/d). Each group was at the 4th week and 10th week after operation, respectively. Results 1. The multicomponent magnetic rods for implantation in the medullary cavity of the distal femoral shaft of rabbits were cylindrical-like with a length of 25.0 mm and a diameter of 7.0 mm. The magnetic field distribution on the surface of the magnetic rod changed between strong and weak phases. The maximum magnetic induction intensity was 40 The results showed that the external bone of the combined magnetic field group basically healed completely and continued with the peripheral bone. Compared with the pulsed electromagnetic field group and the control group, the cortical and subcortical bone regeneration increased significantly, and the trabecular bone morphology and continuity were better. The quantitative analysis of the bone tissue of the combined magnetic field group and the control group showed that the bone defect site was more than that of the combined magnetic field group. Bone volume fraction and trabecular bone number increased significantly (P 0.05), but trabecular bone segregation decreased (P 0.01). There was no significant difference in trabecular bone thickness. Compared with pulsed electromagnetic field group, the number of trabecular bone increased (P 0.05) and trabecular bone segregation decreased (P 0.01) in combined magnetic field group. The gross morphology of cartilage repair in the combined magnetic field group was better than that in the control group (P 0.05) at the 4th week after operation, and there was no significant difference between the combined magnetic field group and the pulsed electromagnetic field group (P 0.05). At the 10th week after operation, the gross morphology score of cartilage repair in the combined magnetic field group was significantly better than that in the control group (P 0.01) and the pulsed electromagnetic field group (P 0.05). At the fourth week, the combined magnetic field group was significantly better than the control group (P 0.05), and there was no significant difference between the combined magnetic field group and the pulsed electromagnetic field group (P 0.05). At the tenth week, the combined magnetic field group was significantly better than the control group (P 0.01), and there was no significant difference between the combined magnetic field group and the pulsed electromagnetic field group (P 0.05). The results showed that GAG levels in control group, pulse electromagnetic field group and combined magnetic field group were 12.26 65507 The content of GAG in pulsed electromagnetic field group and combined magnetic field group was significantly higher than that in control group (P 0.05), and there was no significant difference between the combined magnetic field group and pulsed electromagnetic field group (P 0.05). Electromagnetic field combined with magnetic field can promote bone formation and improve bone microstructure better than single pulse electromagnetic field and control group under the same tissue trauma condition. At the same time, it can enhance the early morphological repair and interface integration of articular cartilage and improve the aminoglycan matrix in cartilage tissue. The results of this study preliminarily confirm the feasibility of the mode of action of implanted magnetic field, and have important research value for local targeted magnetic field therapy and long-term magnetic attachment application of orthopedic implants.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R68
本文编号：2182357
[Abstract]:Objective In order to explore a feasible loading method of magnetic field technology, magnetostatic rods for in vivo implantation were constructed with magnetostat as the original material, and the role of in vivo magnetic source combined with in vitro pulsed electromagnetic field in bone and cartilage tissue repair was clarified. Methods 1. According to the previous research foundation and related papers on magnetic field. Under the guidance of experts in the field of bioengineering, Nd-Fe-B permanent magnets with stable magnetic properties were selected as the basic components to construct the internal magnetic poles of multicomponent magnetic rods implanted in vivo. The external magnetic poles were made of medical titanium alloy materials with good biocompatibility and biomechanical strength. The physical properties of the implanted multivariate magnetic rods were measured by sealed coating. 2. The experimental study of implanted multivariate magnetic rods combined with pulsed electromagnetic field for repairing bone defects in rabbits: All experimental New Zealand white rabbits were selected to establish the femoral condyle defect model. According to the experimental intervention methods, they were divided into combined magnetic field group, pulsed electromagnetic field group and control group, combined magnetic field group. Pulsed electromagnetic field group and control group were implanted non-magnetic titanium alloy rods in the distal femoral shaft medullary cavity of rabbits respectively. Pulsed electromagnetic field group was implanted non-magnetic titanium alloy rods in the distal femoral shaft medullary cavity of rabbits after operation. Pulsed electromagnetic field group was applied in vitro after operation (1h/d). The femurs of each group were harvested at the 5th week after operation. The distal bone samples were grossly, histologically and radiologically assessed; 3. Experimental study of cartilage defect repair with implanted multivariate magnetic rods combined with pulsed electromagnetic field in rabbits: All experimental New Zealand white rabbits were selected to establish the femoral trochlear cartilage defect model, which was also divided into combined magnetic field group, pulsed electromagnetic field group and control group. Pulsed electromagnetic field group and control group were implanted non-magnetic titanium alloy rods in the distal femoral shaft medullary cavity of rabbits. Pulsed electromagnetic field group and control group were implanted non-magnetic titanium alloy rods in the distal femoral shaft medullary cavity of rabbits. Pulsed electromagnetic field group were applied in vitro after operation (1h/d). Each group was at the 4th week and 10th week after operation, respectively. Results 1. The multicomponent magnetic rods for implantation in the medullary cavity of the distal femoral shaft of rabbits were cylindrical-like with a length of 25.0 mm and a diameter of 7.0 mm. The magnetic field distribution on the surface of the magnetic rod changed between strong and weak phases. The maximum magnetic induction intensity was 40 The results showed that the external bone of the combined magnetic field group basically healed completely and continued with the peripheral bone. Compared with the pulsed electromagnetic field group and the control group, the cortical and subcortical bone regeneration increased significantly, and the trabecular bone morphology and continuity were better. The quantitative analysis of the bone tissue of the combined magnetic field group and the control group showed that the bone defect site was more than that of the combined magnetic field group. Bone volume fraction and trabecular bone number increased significantly (P 0.05), but trabecular bone segregation decreased (P 0.01). There was no significant difference in trabecular bone thickness. Compared with pulsed electromagnetic field group, the number of trabecular bone increased (P 0.05) and trabecular bone segregation decreased (P 0.01) in combined magnetic field group. The gross morphology of cartilage repair in the combined magnetic field group was better than that in the control group (P 0.05) at the 4th week after operation, and there was no significant difference between the combined magnetic field group and the pulsed electromagnetic field group (P 0.05). At the 10th week after operation, the gross morphology score of cartilage repair in the combined magnetic field group was significantly better than that in the control group (P 0.01) and the pulsed electromagnetic field group (P 0.05). At the fourth week, the combined magnetic field group was significantly better than the control group (P 0.05), and there was no significant difference between the combined magnetic field group and the pulsed electromagnetic field group (P 0.05). At the tenth week, the combined magnetic field group was significantly better than the control group (P 0.01), and there was no significant difference between the combined magnetic field group and the pulsed electromagnetic field group (P 0.05). The results showed that GAG levels in control group, pulse electromagnetic field group and combined magnetic field group were 12.26 65507 The content of GAG in pulsed electromagnetic field group and combined magnetic field group was significantly higher than that in control group (P 0.05), and there was no significant difference between the combined magnetic field group and pulsed electromagnetic field group (P 0.05). Electromagnetic field combined with magnetic field can promote bone formation and improve bone microstructure better than single pulse electromagnetic field and control group under the same tissue trauma condition. At the same time, it can enhance the early morphological repair and interface integration of articular cartilage and improve the aminoglycan matrix in cartilage tissue. The results of this study preliminarily confirm the feasibility of the mode of action of implanted magnetic field, and have important research value for local targeted magnetic field therapy and long-term magnetic attachment application of orthopedic implants.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R68
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