股骨头坏死早期大鼠股骨头骨组织中基质金属蛋白酶及其相关抑制剂的表达

发布时间：2018-08-15 14:24

【摘要】：目的观察大鼠股骨头骨组织中基质金属蛋白酶(MMPs)及其抑制剂的表达变化,探索股骨头坏死形成与应用糖皮质激素之间的相互关系。方法 20只SD大鼠,雌雄各半,分为2组(n=10)。激素模型组肌注醋酸氢化可的松,2次/周;对照组给予等量生理盐水。4周后,取各组大鼠左侧股骨头的骨组织进行HE染色确定股骨头坏死病变情况;采用RT-PCR及Western blot技术分别检测大鼠股骨头组织中MMP-1、MMP-2、MMPs组织抑制剂-1(TIMP-1)、TIMP-2的mRNA和蛋白表达变化。结果激素模型组MMP-1、MMP-2mRNA及蛋白表达较对照组升高,TIMP-1、TIMP-2的mRNA及蛋白表达降低(均为P0.05)。结论股骨头坏死早期大鼠股骨头骨组织中MMPs表达升高,其相关抑制剂表达降低,推测糖皮质激素导致股骨头坏死可能与其对体内MMPs及其相关抑制剂的调控有关。
[Abstract]:Objective to observe the expression of matrix metalloproteinase (MMPs) and its inhibitor in the femoral head of rats and to explore the relationship between the formation of necrosis of femoral head and glucocorticoid application. Methods Twenty SD rats, half male and half female, were divided into 2 groups (n = 10). In the hormone model group, hydrocortisone acetate was injected intramuscularly twice a week, and in the control group, the bone tissue of the left femoral head was taken for HE staining to determine the lesion of femoral head necrosis after the control group was given the same amount of normal saline for 4 weeks. RT-PCR and Western blot techniques were used to detect the expression of mRNA and protein in the tissue inhibitor of MMP-1, MMP-2MMPs (TIMP-1) and TIMP-2 in the femoral head of rats. Results compared with the control group, the expression of MMP-1 and MMP-2 mRNA and protein in the steroid model group was significantly higher than that in the control group. The expression of mRNA and protein of TIMP-2 was decreased (P0.05). Conclusion in the early stage of femoral head necrosis, the expression of MMPs increased and the expression of related inhibitors decreased in the femoral head of rats. It is speculated that glucocorticoid induced femoral head necrosis may be related to the regulation of MMPs and its related inhibitors in vivo.
【作者单位】：新乡医学院第一附属医院骨外科;深圳大学附属罗湖医院骨科;中国科学院深圳先进技术研究院医药所推行中心;
【分类号】：R681.8

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