督脉电针结合超短波疗法对大鼠脊髓全横断损伤后脑源性神经生长因子和神经轴突生长抑制剂-A表达的影响

发布时间：2018-08-17 11:01

【摘要】：目的:通过检测大鼠脊髓损伤(SCI)后运动功能恢复情况和脑源性神经生长因子(BDNF)、神经轴突生长抑制剂-A(Nogo-A)的表达来探讨联合应用督脉电针和超短波疗法对SCI的作用机制。方法:复制并评价脊髓全横断损伤大鼠模型,100只雌性大鼠随机分为5组:假手术组、SCI组、SCI+督脉电针组、SCI+超短波组、SCI+督脉电针+超短波组(n=20),检测各组7d、14d、21d、28d这4个时间点BDNF和Nogo-A表达,对各组大鼠进行BBB评分。结果:与模型组相比,各治疗组的BBB评分显著增加,联合组在术后第7d、14d、21d、28d较超短波组和督电组BBB评分显著增加,具有显著性差异(P0.01)。与模型组相比,各治疗组BDNF的表达均显著增加,Nogo-A的表达均显著减少,联合组在术后第7d、14d、21d、28d较超短波组和督电组BDNF的表达显著增加,Nogo-A的表达显著减少,具有显著性差异(P0.01)。结论:(1)联合应用督脉电针与超短波治疗,能够提高SCI后BDNF的表达及抑制Nogo-A的表达来促进神经的修复。(2)联合疗法对大鼠运动功能恢复更佳。
[Abstract]:Aim: to investigate the mechanism of combined use of du meridian electroacupuncture and ultrashort wave therapy in detecting the recovery of motor function after spinal cord injury (SCI) and the expression of brain-derived nerve growth factor (BDNF),) nerve axon growth inhibitor -A (Nogo-A) in rats with spinal cord injury (sci). Methods: a total of 100 female rats with spinal cord transection injury were randomly divided into five groups: sham operation group (sci group), sci du pulse electroacupuncture group (sci ultrashort wave group), sci supernatant pulse electroacupuncture (sci) ultrashort wave group (n = 20), and the expression of BDNF and Nogo-A at four time points (7 d, 14 d, 21 d, 28 d). BBB score was performed on each group of rats. Results: compared with the model group, the BBB score of each treatment group was significantly increased, and the BBB score of the combined group was significantly higher than that of the ultrashort wave group and the governor group on the 7th day, 14d, 21d and 28d after operation (P0.01). Compared with the model group, the expression of BDNF in each treatment group was significantly increased and the expression of Nogo-A was significantly decreased in the combined group. The expression of BDNF in the combined group was significantly lower than that in the ultrashort wave group and the control group on the 14th day and 21st day after operation (P0.01). Conclusion: (1) combined therapy of du meridian electroacupuncture and ultrashort wave can increase the expression of BDNF and inhibit the expression of Nogo-A after SCI to promote nerve repair. (2) the combined therapy can improve the recovery of motor function in rats.
【作者单位】： 锦州医科大学附属第三医院;
【基金】：辽宁省省级科研项目下分项(KK0457)
【分类号】：R651.2
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本文编号：2187394