Gab2、IRX2表达量与骨肉瘤组织中细胞生长、迁移行为的相关性

发布时间：2018-08-23 10:13

【摘要】：目的:研究骨肉瘤组织中Gab2、IRX2表达量与细胞生长、迁移行为的相关性。方法:选择在我院手术切除的29例骨肉瘤组织和22例骨样骨瘤组织作为检测标本,抽提RNA并测定Gab2、IRX2及细胞生长、迁移相关基因的mRNA表达量,抽提蛋白并测定Gab2、IRX2及细胞生长、迁移相关基因的蛋白表达量。结果:骨肉瘤组织中Gab2、IRX2的mRNA含量和蛋白含量均显著高于骨样骨瘤组织;骨肉瘤组织中TAp73、Beclin1、Caspase-1的mRNA含量和蛋白含量均显著低于骨样骨瘤组织且与Gab2、IRX2的mRNA含量和蛋白含量呈负相关,STMN1、Survivin的mRNA含量和蛋白含量均显著高于骨样骨瘤组织且与Gab2、IRX2、CEACAM6、CD44v6、MMP-9的mRNA含量和蛋白含量呈正相关。结论:骨肉瘤组织中高表达的Gab2和IRX2能够抑制原癌基因的表达、增加促增殖和迁移基因的表达,进而促进骨肉瘤组织中细胞的生长、迁移。
[Abstract]:Aim: to study the correlation between the expression of Gab2 ~ (2 +) IRX _ 2 and cell growth and migration in osteosarcoma. Methods: RNA was extracted from 29 cases of osteosarcoma and 22 cases of osteoid osteoma from our hospital. The expression of mRNA, protein and cell growth were measured. Protein expression of translocation related genes. Results: the contents of mRNA and protein in osteosarcoma tissue were significantly higher than those in osteoid osteoma tissue. The content of mRNA and protein in osteosarcoma tissue was significantly lower than that in osteoid osteoma tissue. The mRNA content and protein content of survivin were significantly higher than those in osteoid osteosarcoma tissue and the mRNA of mRNA with Gab2IRX2 CD44v6MMP-9 was significantly higher than that of osteoid osteoma tissue. There was a positive correlation between the amount of protein and the content of protein. Conclusion: the overexpression of Gab2 and IRX2 in osteosarcoma can inhibit the expression of proto-oncogene, increase the expression of pro-proliferation and migration genes, and promote cell growth and migration in osteosarcoma.
【作者单位】：湖北谷城县人民医院肿瘤科;
【基金】：湖北省卫生和计划生育委员会科研项目(WJ2015MB114)~~
【分类号】：R738.1

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