胸腺素β4对增生性瘢痕基质金属蛋白酶-2、9表达作用的影响
发布时间：2018-08-24 11:46
【摘要】：目的:研究胸腺素β4(thymosin beta4)干预后的人增生性成纤维细胞(hypertrophicscar fibroblasts HSFB)的基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶(MMP-9)的表达水平,探讨胸腺素β4对增生性瘢痕可能的作用机制,提出将胸腺素β4可用于治疗增生性瘢痕的理论依据。方法:选取从我院烧伤科和整形科病人烫伤或烧伤的病人,入选标准:1、烧伤半年后,2、瘢痕出现较严重的挛缩,3、未使用其他药物亦未进行其他治疗,3、有手术切除指征并于我院进行手术,4、患者和家属自愿签署相关知情同意书。术中切除患者瘢痕组织后,将患者瘢痕组织采用组织块法培养增生性瘢痕成纤维细胞。取细胞3-6代细胞实验,设对照组未加入胸腺素β4,其余实验组分别加入0.05μg/m L,0.1μg/m L,1.0μg/m L,5.0μg/m L的胸腺素β4进行干预24小时。(1)通过逆转录-聚合酶链反应(RT-PCR)法检测胸腺素β4对HSFB基质金属蛋白酶MMP-2 m RNA和MMP-9 m RNA的表达;(2)通过免疫印迹法(Western blot)检测HSFB基质金属蛋白酶MMP-2和MMP-9蛋白的表达。结果:经过24小时各浓度的胸腺素β4作用后:(1)MMP-2 m RNA和MMP-9 m RNA表达灰度比值较实验组均增加,且以5.0μg/m L组增高程度最甚,差异有统计学意义(P0.05)(2)MMP-2和MMP-9蛋白表达灰度比值较对照组均增加,且以5.0μg/m L组增高程度最甚,差异有统计学意义(P0.05)。结论:胸腺素β4能浓度依赖性地增加HSFB中MMP-2,MMP-9因子的表达。由此可推断:胸腺素β4可能通过这种方式来影响增生性瘢痕的形成。
[Abstract]:Objective: to investigate the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase (MMP-9) in human proliferative fibroblasts (hypertrophicscar fibroblasts HSFB) after intervention of thymosin 尾 4 (thymosin beta4), and to explore the possible mechanism of thymosin 尾 4 on hypertrophic scar. It was suggested that thymosin 尾 4 could be used in the treatment of hypertrophic scar. Methods: the patients who were scalded or burned from the Department of Burn and plastic surgery in our hospital were selected. Standard 1: 1, 2 months after burn, more severe contracture appeared in scar, no other drugs were used and other treatment was not carried out. There were indications of surgical excision and operation in our hospital. Patients and their families voluntarily signed the relevant informed consent. The hypertrophic scar fibroblasts were cultured by tissue mass method after the scar tissue was excised. The 3-6 passage cells were taken from the cells. Thymosin 尾 4 was added to the control group without thymosin 尾 4. The other experimental groups were treated with 0. 0 渭 g / mL Thymosin 尾 4 and 0. 0 渭 g / mL Thymosin 尾 4 for 24 hours. (1) Thymosin 尾 4 was detected by reverse transcription-polymerase chain reaction (RT-PCR) for HSFB matrix metalloproteinase MMP-2 m RNA. (2) the expression of HSFB matrix metalloproteinase MMP-2 and MMP-9 protein was detected by Western blot (Western blot). Results: after 24 hours of thymosin 尾 4 treatment: (1) the gray level ratio of MMP-2 m RNA and MMP-9 m RNA was increased compared with the experimental group, especially in the 5.0 渭 g / mL group. The difference was statistically significant (P0.05) (2) the gray ratio of MMP-2 and MMP-9 protein expression was higher than that of the control group, and the most significant increase was in the 5.0 渭 g / mL group (P0.05). Conclusion: thymosin 尾 4 can increase the expression of MMP-2,MMP-9 factor in HSFB in a concentration dependent manner. It can be concluded that thymosin 尾 4 may affect the formation of hypertrophic scar in this way.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R622
[Abstract]:Objective: to investigate the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase (MMP-9) in human proliferative fibroblasts (hypertrophicscar fibroblasts HSFB) after intervention of thymosin 尾 4 (thymosin beta4), and to explore the possible mechanism of thymosin 尾 4 on hypertrophic scar. It was suggested that thymosin 尾 4 could be used in the treatment of hypertrophic scar. Methods: the patients who were scalded or burned from the Department of Burn and plastic surgery in our hospital were selected. Standard 1: 1, 2 months after burn, more severe contracture appeared in scar, no other drugs were used and other treatment was not carried out. There were indications of surgical excision and operation in our hospital. Patients and their families voluntarily signed the relevant informed consent. The hypertrophic scar fibroblasts were cultured by tissue mass method after the scar tissue was excised. The 3-6 passage cells were taken from the cells. Thymosin 尾 4 was added to the control group without thymosin 尾 4. The other experimental groups were treated with 0. 0 渭 g / mL Thymosin 尾 4 and 0. 0 渭 g / mL Thymosin 尾 4 for 24 hours. (1) Thymosin 尾 4 was detected by reverse transcription-polymerase chain reaction (RT-PCR) for HSFB matrix metalloproteinase MMP-2 m RNA. (2) the expression of HSFB matrix metalloproteinase MMP-2 and MMP-9 protein was detected by Western blot (Western blot). Results: after 24 hours of thymosin 尾 4 treatment: (1) the gray level ratio of MMP-2 m RNA and MMP-9 m RNA was increased compared with the experimental group, especially in the 5.0 渭 g / mL group. The difference was statistically significant (P0.05) (2) the gray ratio of MMP-2 and MMP-9 protein expression was higher than that of the control group, and the most significant increase was in the 5.0 渭 g / mL group (P0.05). Conclusion: thymosin 尾 4 can increase the expression of MMP-2,MMP-9 factor in HSFB in a concentration dependent manner. It can be concluded that thymosin 尾 4 may affect the formation of hypertrophic scar in this way.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R622
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