Toll样受体4在胆道损伤修复过程中的表达及意义

发布时间：2018-08-26 07:43

【摘要】：目的:探讨Toll样受体4(TLR4)在胆道损伤修复过程中的作用。方法:用免疫组化检测12例良性胆道狭窄胆管组织与4例正常胆管组织中TLR4的表达。用钳夹法在TLR4基因缺陷(TLR4~(-/-))小鼠和野生(TLR4~(+/+))小鼠上建立胆道损伤修复模型,并设各自的假手术对照,术后48h观察各组肝脏及胆管组织病理学变化及肝功能情况。结果:TLR4主要表达于胆管内皮细胞,良性胆道狭窄胆管壁表达阳性率明显高于正常胆管组织(83.33%vs.25.00%,P0.01)。两个假手术组小鼠术后肝脏及胆管组织均未见病理改变,TLR4~(-/-)与TLR4~(+/+)模型组小鼠均出现明显的胆道及肝脏损伤,但前者较后者明显减轻;与各自的对照组比较,TLR4~(-/-)与TLR4~(+/+)模型组小鼠血清谷丙转氨酶、总胆红素、直接胆红素水平均明显升高(均P0.05),但前者各项指标的升高程度均明显低于后者(均P0.05)。结论:TLR4可能通过参与胆管上皮细胞的天然免疫应答,并启动一系列炎症因子的表达,促使成纤维细胞增殖,在胆管良性狭窄中起到重要作用。
[Abstract]:Objective: to investigate the role of Toll like receptor 4 (TLR4) in the repair of biliary tract injury. Methods: immunohistochemical method was used to detect the expression of TLR4 in 12 cases of benign bile duct stricture and 4 cases of normal bile duct. Bile duct injury repair models were established in TLR4~ (-r -) mice and TLR4~ (/) mice by clamp method. The pathological changes and liver function of liver and bile duct were observed 48 hours after operation. Results the positive expression rate of TLR4 in benign bile duct stricture was significantly higher than that in normal bile duct (83.33vs.25.00 P0.01). No pathological changes were found in the liver and bile duct tissues of the two sham-operated mice after operation. Both TLR4- /-and TLR4~ (/) model mice showed obvious bile duct and liver injury, but the former was significantly less than the latter. The levels of serum alanine aminotransferase, total bilirubin and direct bilirubin in TLR4- /-and TLR4~ (/) group were significantly higher than those in control group (P0.05), but the former was significantly lower than the latter (P0.05). Conclusion: TLR4 may play an important role in benign bile duct stenosis by taking part in the innate immune response of bile duct epithelial cells and initiating the expression of a series of inflammatory factors to promote the proliferation of fibroblasts.
【作者单位】：甘肃省人民医院普外一科;
【分类号】：R657.4

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