乌头注射液对兔膝关节骨性关节炎关节液中IL-1β及IL-18含量的影响

发布时间：2018-08-27 13:35

【摘要】：目的:通过本课题的实验研究,探讨乌头注射液对兔膝关节骨性关节炎关节液中IL-1β、IL-18含量的影响,为进一步的临床应用奠定基础。方法:1、采选用2%w/v的木瓜蛋白酶溶液与0.03mol/L的L-半胱氨酸溶液比例为2:1的混合溶液,按0.1ml/kg注射至新西兰大耳白兔双后膝关节腔内,建立兔膝关节骨性关节炎模型。2、将20只雄性新西兰大耳白兔按体重编号随机分为:空白组、模型组、阳性药对照组、实验组。除空白组外,其它组进行造模,2周后行X线观察造模是否成功。造模成功后,开始治疗,实验组注射乌头注射液0.2ml,阳性药对照组注射玻璃酸钠注射液0.2ml,模型组注射生理盐水0.2ml,空白组不给予任何处理。治疗结束1周后取双后膝关节关节液,检测关节液中IL-1β、IL-18的含量;解剖膝关节肉眼观察;经HE染色显微镜下观察。3、采用统计学处理分析软件SPSS19.0对数据进行处理,若P0.05即认为检验结果有统计学意义。计数资料用构成比或率的形式表示,需采用x2检验;计量资料用x±s形式表示,需采用t检验。结果:1、肉眼观察软骨结果:模型组与空白组比较发现软骨表面有多处裂纹或缺损,关节边缘几乎全有骨赘形成,且多呈黄色;阳性药对照组和实验组相似,两者与模型组比较发现软骨表面裂纹或缺损较少,少有骨赘形成,且多呈白色。2、显微镜下观察Mankin评分结果:模型组评分高于空白组(P0.05),差异有统计学意义;阳性药对照组和实验组评分均低于模型组(P0.05),差异有统计学意义。3、模型组关节液中IL-1β、IL-18的含量与空白组比较均明显增多(P0.05),差异有统计学意义,说明本实验造模成功;阳性药对照组和实验组关节液中IL-1β、IL-18的含量与模型组比较均明显降低(P0.05),差异有统计学意义。结论:乌头注射液不仅起到保护兔膝关节软骨的作用而且能够降低兔膝关节骨性关节炎关节液中IL-1β、IL-18的含量,达到消除炎症的目的。
[Abstract]:Objective: to investigate the effect of aconitum injection on the content of IL-1 尾 -IL-18 in the articular fluid of knee joint osteoarthritis of rabbits, so as to lay a foundation for further clinical application. Methods the mixture of 2%w/v 's papain solution and 0.03mol/L 's L-cysteine solution (2:1) was used to inject 0.1ml/kg into the posterior knee joint of New Zealand big ear white rabbit. A rabbit model of osteoarthritis of knee joint was established. Twenty male New Zealand white rabbits were randomly divided into blank group, model group, positive drug control group and experimental group according to their body weight. In addition to the blank group, the other groups were given X-ray after 2 weeks to observe whether the model was successful. After the model was established successfully, the experimental group was injected with aconitum injection 0.2 ml, the positive control group with sodium hyaluronate injection 0.2 ml, the model group with physiological saline 0.2 ml, the blank group without any treatment. After 1 week of treatment, the contents of IL-1 尾 -IL-18 in the joint fluid were measured, the naked eyes of the knee joint were dissected, observed under the microscope of HE staining, and the data were processed by the statistical analysis software SPSS19.0. If P0.05 that the test results were statistically significant. The counting data is expressed in the form of composition ratio or rate, which needs x2 test, and the measurement data is expressed in the form of x 卤s, and the t test is used. Results: compared with the blank group, the model group found that there were many cracks or defects on the cartilage surface, almost all the osteophytes formed on the articular edge, and most of them were yellow, while the positive drug control group was similar to the experimental group. Compared with the model group, it was found that the cartilage surface cracks or defects were less, few osteophytes formed, and most of them were white. The Mankin score of the model group was higher than that of the blank group (P0.05), and the difference was statistically significant. The score of positive drug control group and experimental group were lower than that of model group (P0.05), the difference was statistically significant. The content of IL-1 尾 -IL-18 in joint fluid of model group was significantly higher than that of blank group (P0.05), the difference was statistically significant. The content of IL-1 尾 -IL-18 in the joint fluid of the positive control group and experimental group was significantly lower than that of the model group (P0.05), and the difference was statistically significant. Conclusion: aconitum injection can not only protect the articular cartilage of rabbit knee joint, but also reduce the content of IL-1 尾 -IL-18 in the articular fluid of rabbit knee osteoarthritis, so as to eliminate inflammation.
【学位授予单位】：黑龙江省中医药科学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R684.3

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