右美托咪定对脂多糖诱导的脓毒症大鼠全身炎症反应和肺损伤的影响

发布时间：2018-08-29 18:57

【摘要】：目的观察脂多糖(LPS)对大鼠血浆和肺组织中的炎性因子和Toll样受体4(TLR4)表达的影响及右美托咪定的干预作用。方法雄性SD大鼠40只,250~300g,随机分为四组。Control组(n=10)生理盐水1ml·kg~(-1)·h~(-1)大鼠尾静脉泵注6h;DEX组(n=10)右美托咪定(负荷量6.5μg·kg~(-1)·h~(-1),10min;5μg·kg~(-1)·h~(-1)维持)大鼠尾静脉泵注6h;LPS组(n=10)经大鼠尾静脉注射7.5mg/kg的LPS后继续生理盐水泵注6h;LPS+DEX组(n=10)经大鼠尾静脉注射7.5mg/kg的LPS后泵注右美托咪定6h。以6h为实验终结点,并于此时行右心室取血和肺组织标本的制备。采用ELISA法检测血浆IL-1、IL-6、TNF-α浓度,Western blot法检测肺组织TLR4、髓样分化因子88(MyD88)、NF-κB蛋白含量;检测大鼠肺组织湿/干比(W/D);并用Murakami法评测肺损伤程度。结果与Control组比较,LPS组大鼠血浆IL-1、IL-6和TNF-α浓度明显升高,肺组织中的TLR4、MyD88、NF-κB蛋白含量明显升高(P0.01),肺W/D明显升高;LPS+DEX组上述指标差异均无统计学意义。Control组和DEX组未见明显肺损伤,LPS组肺间质水肿、炎性细胞浸润明显,LPS+DEX组肺损伤程度明显减轻(P0.01)。结论 LPS的刺激可以明显升高大鼠血浆中炎性因子以及肺组织中TLR4的表达水平,右美托咪定的干预可以减轻这一趋势,缓解大鼠的全身炎症和肺水肿的程度。
[Abstract]:Objective to observe the effect of lipopolysaccharide (LPS) on the expression of inflammatory factors and Toll like receptor 4 (TLR4) in plasma and lung tissue of rats and the intervention of dexmetomidine. Methods 40 male SD rats were randomly divided into four groups: control group (n = 10) normal saline 1ml kg~ (-1) h ~ (-1) rats were injected by caudal vein infusion of dexmetomidine (kg~ (-1) h ~ (-1) at a dose of 6.5 渭 g kg~ (-1) h ~ (-1) for 10 min, and rats in the control group (n = 10) were injected with LPS of 7.5mg/kg via caudal vein. The rats in the LPS-10 group were injected with LPS of 7.5mg/kg via caudal vein for 6 h after infusion of normal saline for 6 h. At the end point of 6 h, the right ventricular blood and lung tissue samples were prepared. The concentration of plasma IL-1,IL-6,TNF- 伪 was determined by ELISA and the content of TLR4, myeloid differentiation factor 88 (MyD88) NF- 魏 B in lung tissue was detected by Western blot, the wet / dry ratio (WR) of lung tissue was measured by Murakami method, and the degree of lung injury was evaluated by Murakami method. Results compared with Control group, the concentration of IL-1,IL-6 and TNF- 伪 in plasma of rats in lipopolysaccharide group was significantly higher than that in control group. The content of TLR4,MyD88,NF- 魏 B protein in lung tissue was significantly increased (P0.01). There was no significant difference in the above indexes between the two groups. There was no significant difference in pulmonary interstitial edema between control group and DEX group. The degree of lung injury was obviously alleviated in DEX group (P0.01). Conclusion the stimulation of LPS can significantly increase the level of inflammatory factors in plasma and the expression of TLR4 in lung tissue of rats. The intervention of dexmetidine can alleviate this trend and alleviate the degree of systemic inflammation and pulmonary edema in rats.
【作者单位】：南京医科大学附属松江中心医院麻醉科;
【基金】：上海市医学会麻醉学分会新晨杯青年麻醉医师培育基金(2014)
【分类号】：R614

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