竹叶黄酮缓解异氟醚引起的老年大鼠认知功能障碍

发布时间：2018-08-31 11:50

【摘要】：研究竹叶黄酮对异氟醚吸入诱发老年大鼠神经细胞凋亡及认知功能障碍的影响.分离培养原代海马神经细胞,暴露于体积分数为2%异氟醚6 h后,分别给予50、80及100 mg/L竹叶黄酮处理,MTT法检测细胞增殖能力,流式细胞仪检测细胞凋亡情况.大鼠经吸入1.4%体积的异氟醚2 h后,分别于1~5 d连续腹腔注射25、50、100 mg/kg竹叶黄酮,于第6天进行水迷宫实验检测大鼠空间学习记忆能力,ELISA法检测海马组织匀浆中的Aβ1-42、TNF-α、INF-γ含量.结果发现,竹叶黄酮可显著促进海马神经细胞的增殖,并且抑制异氟醚所致的海马神经细胞凋亡.与异氟醚单独处理组相比较,各浓度竹叶黄酮可显著降低大鼠逃避潜伏期并增加大鼠过台次数,ELISA结果显示竹叶黄酮可显著抑制海马组织中Aβ1-42、TNF-α及INF-γ的表达.表明竹叶黄酮可增强大鼠抗炎能力,抑制海马组织中Aβ1-42的产生,从而抑制异氟醚所致的老年大鼠神经损伤及认知功能障碍.
[Abstract]:To study the effects of bamboo leaf flavonoids on neuronal apoptosis and cognitive impairment induced by isoflurane inhalation in aged rats. Primary hippocampal nerve cells were isolated and cultured. After exposure to 2% isoflurane for 6 h, 50 or 100 mg/L bamboo leaf flavonoids were used to detect cell proliferation, and flow cytometry was used to detect cell apoptosis. Rats were inhaled with 1.4% isoflurane for 2 h, and 25 50 100 mg/kg bamboo leaf flavonoids were injected intraperitoneally at 1 and 5 days respectively. On the 6th day, the spatial learning and memory abilities of rats were measured by water maze test and the content of A 尾 1-42 TNF- 伪 -INF- 纬 in hippocampus homogenate was detected by Elisa. The results showed that bamboo leaf flavonoids could significantly promote the proliferation of hippocampal neurons and inhibit the apoptosis of hippocampal neurons induced by isoflurane. Compared with the isoflurane treatment group, each concentration of bamboo leaf flavonoids significantly decreased the escape latency and increased the number of rats passing through the stage. Elisa results showed that bamboo leaf flavonoids could significantly inhibit the expression of A 尾 1-42 TNF- 伪 and INF- 纬 in hippocampal tissue. The results showed that the flavonoids of bamboo leaves could enhance the anti-inflammatory ability and inhibit the production of A 尾 1-42 in the hippocampus of rats, thus inhibiting the nerve damage and cognitive impairment induced by isoflurane in the aged rats.
【作者单位】：河南省南阳市中心医院麻醉科一部;河南省南阳市南阳医专一附院麻醉科;
【分类号】：R614

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