周围神经损伤后背根神经节神经元及M1、M2型巨噬细胞变化

发布时间：2018-09-03 14:26

【摘要】：目的观察大鼠坐骨神经损伤后背根神经节神经元数目变化及M1、M2型巨噬细胞浸润情况。方法暴露大鼠右侧坐骨神经,于梨状肌下缘0.5cm处切断并原位吻合,左侧作为空白对照。术后1d、3d、1周、2周、4周、6周、8周剪取两侧背根神经节,石蜡切片后行甲苯胺蓝染色及免疫组织化学染色,观察背根神经节神经元数目及M1、M2型巨噬细胞的浸润情况。结果与左侧相比,术后1d、3d、1周、2周、4周、6周、8周时,右侧背根神经节神经元数目分别减少0.2%、1.9%、8.6%、23.0%、29.0%、31.0%、31.0%。术后1d右侧背根神经节内可见少量M1型巨噬细胞浸润,术后3d时M1型巨噬细胞数增多,术后1周时达顶峰,之后开始逐渐减少,至术后6周,再无M1型巨噬细胞浸润;M2型巨噬细胞于术后1周开始浸润,术后2周时右侧背根神经节内M2型巨噬细胞数达顶峰,之后逐渐减少,术后8周无M2型巨噬细胞浸润。左侧背根神经节内始终未见M1、M2型巨噬细胞浸润。结论大鼠坐骨神经损伤后,相应背根神经节感觉神经元数目在4周内逐渐减少,损伤初期以M1型巨噬细胞浸润为主,损伤中期以M2型巨噬细胞浸润为主。
[Abstract]:Objective to observe the number of dorsal root ganglion neurons and M 1 M 2 macrophage infiltration after sciatic nerve injury in rats. Methods the right sciatic nerve of the rat was exposed and transected at the inferior margin of piriformis muscle 0.5cm and anastomosed in situ. The left side was used as a blank control. Bilateral dorsal root ganglion (DRG) was cut at 1 day, 3 d, 1 week, 2 weeks, 4 weeks and 6 weeks and 8 weeks. Toluidine blue staining and immunohistochemical staining were performed after paraffin section. The number of dorsal root ganglion neurons and the infiltration of M 1 / M 2 macrophages were observed. Results compared with the left side, the number of neurons in the right dorsal root ganglion decreased by 0.2 and 1.9 on the 1st day and 3rd day after the operation from 1 week to 2 weeks and 4 weeks and 6 weeks and 8 weeks, respectively. A small number of M1 type macrophages were observed in the right dorsal root ganglion on the 1st day after operation. The number of M1 type macrophages increased at 3 days after operation, peaked at 1 week after operation, and then gradually decreased, and then decreased gradually to 6 weeks after operation. No M 1 macrophages infiltrated. M 2 macrophages began to infiltrate 1 week after operation. The number of M 2 type macrophages in the right dorsal root ganglion peaked at 2 weeks after operation, then gradually decreased, and no M 2 type macrophage infiltration was observed at 8 weeks after operation. No M 1 M 2 macrophage infiltration was found in the left dorsal root ganglion. Conclusion after sciatic nerve injury, the number of sensory neurons in the corresponding dorsal root ganglion decreased gradually within 4 weeks. M1 type macrophage infiltration was dominant in the early stage of injury and M2 type macrophage infiltration in the middle stage of injury.
【作者单位】：青岛大学附属医院创伤外科;青岛大学医学院免疫学教研室;
【分类号】：R651.3

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