十全大补汤对乳腺癌患者术后早期恢复及切口引流液血管生成因子的影响

发布时间：2018-09-06 18:37

【摘要】：目的:观察十全大补汤对乳腺癌患者术后早期恢复的作用,并探讨对切口引流液血管生成因子的影响以及引流液对乳腺癌细胞MDA-MB-231增殖、迁移和侵袭能力的作用。方法:将61例乳腺癌病例随机分为对照组30例和治疗组31例,两组术后均采用西医常规治疗,治疗组于术后第1天起服用十全大补汤,连续7 d;观察两组患者治疗前后中医证候疗效、切口引流时间及不同时点引流量,CCK-8法检测引流液对MDA-MB-231细胞增殖的影响、Transwell法检测细胞迁移、侵袭能力的变化,蛋白质芯片法筛选两组引流液中血管生成因子的表达。结果:(1)十全大补汤组中医证候疗效总有效率为89.66%,高于对照组的63.33%(P0.05)。(2)十全大补汤组切口引流时间短于对照组引流时间,分别为(12.48±2.11)d、(14.23±1.94)d(P0.05)。两组引流量均呈下降趋势,其中第7天十全大补汤组引流量低于对照组(P0.05)。(3)引流液对MDA-MB-231细胞有促增殖作用,十全大补汤组引流液对MDA-MB-231乳腺癌细胞的促增殖作用低于对照组(P0.05)。(4)Transwell结果发现引流液能促进MDA-MB-231细胞迁移和侵袭能力,而十全大补汤组的引流液促迁移和侵袭能力较对照组降低(P0.05)。(5)人血管生成因子抗体芯片筛选发现43种血管生成因子在引流液中均有表达,部分促血管生成因子上调≥2倍,其中十全大补汤组IL-8、Leptin的表达水平低于对照组(P0.05)。结论:十全大补汤能改善乳腺癌患者术后早期气血两虚症候,有效缩短术区引流时间、减少术区引流量;切口引流液能促进MDA-MB-231的增殖、迁移及侵袭,十全大补汤可能通过降低引流液中IL-8、Le Ptin等促血管生成因子的表达进一步抑制引流液对MDA-MB-231细胞的促增殖、迁移及侵袭能力。
[Abstract]:Objective: to observe the effect of Shiquandabu decoction on the early postoperative recovery of breast cancer patients, and to explore the effect on the angiogenesis factor of incision drainage fluid and the effect of drainage fluid on the proliferation, migration and invasion of breast cancer cell line MDA-MB-231. Methods: 61 cases of breast cancer were randomly divided into control group (n = 30) and treatment group (n = 31). After 7 days of treatment, the effects of TCM syndrome, incision drainage time and drainage flow at different time points on the proliferation of MDA-MB-231 cells were detected by CCK-8 method and the changes of cell migration and invasion ability were detected by Transwell method. The expression of angiogenic factor in drainage fluid was screened by protein chip method. Results: (1) the total effective rate of TCM syndromes in the Shiquanduanbu decoction group was 89.66, which was higher than that in the control group (63.33%, P0.05). (2). The drainage time of incision in the Shiquanduanbu decoction group was shorter than that in the control group, which was (12.48 卤2.11) d, (14.23 卤1.94) d (P 0.05). The drainage flow of the two groups showed a downward trend, and on the 7th day, the drainage volume of the 10 Dabu decoction group was lower than that of the control group (P0.05). (3), and the drainage fluid had the effect of promoting the proliferation of MDA-MB-231 cells. The effect of drainage fluid on proliferation of MDA-MB-231 breast cancer cells in Shiquandabu decoction group was lower than that in control group (P0.05). (4) Transwell. The results showed that the drainage fluid could promote the migration and invasion of MDA-MB-231 cells. Compared with the control group (P0.05). (5), 43 kinds of angiogenic factors were found to be expressed in the drainage fluid, and some angiogenic factors were up-regulated more than 2 times, compared with the control group (P0.05). (5). The expression level of IL-8,Leptin in Shiquandabu decoction group was lower than that in control group (P0.05). Conclusion: Shiquandabu decoction can improve the symptoms of deficiency of qi and blood, shorten the drainage time and reduce the drainage flow in the early postoperative stage of breast cancer patients, and the drainage fluid of incision can promote the proliferation, migration and invasion of MDA-MB-231. Shiquandabu decoction could further inhibit the proliferation, migration and invasion of MDA-MB-231 cells by reducing the expression of angiogenic factors such as IL-8,Le Ptin in drainage fluid.
【作者单位】：上海中医药大学附属普陀医院中医肿瘤科;
【基金】：国家自然科学基金项目(81273733)
【分类号】：R737.9

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