A型肉毒毒素对增生性瘢痕成纤维细胞的抑制作用及机制

发布时间：2018-09-08 20:14

【摘要】：目的探讨A型肉毒毒素(BTXA)对增生性瘢痕成纤维细胞的抑制作用及机制。方法 0(对照组)、0.2、0.4、0.8U/mL的BTXA作用于增生性瘢痕成纤维细胞48h,噻唑蓝(MTT)法检测细胞活力,Hoechst染色检测细胞凋亡,流式细胞术检测细胞周期,免疫印迹法分析细胞周期蛋白D1(Cyclin D1)、增殖细胞核抗原(PCNA)、磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)通路的激活状况。结果与对照组(0.75±0.07)比较,0.2、0.4、0.8U/mL的BTXA(0.59±0.06、0.43±0.04、0.34±0.03)可明显降低增生性成纤维细胞活力;与对照组[(2.38±0.24)%]比较,可显著提高细胞凋亡率[(15.79±1.54)%、(27.32±2.69)%、(38.46±3.90)%];下调Cyclin D1及PCNA表达,降低PI3K表达及AKT磷酸化水平,并使细胞周期阻滞在G1期,差异均具有统计学意义(P0.05)。结论 BTXA可通过阻断PI3K/AKT信号通路及细胞周期蛋白表达,进而抑制增生性细胞增殖。
[Abstract]:Objective to investigate the inhibitory effect and mechanism of botulinum toxin type A (BTXA) on hypertrophic scar fibroblasts. Methods 0 (control group) 0.4U / mL BTXA was used to treat hypertrophic scar fibroblasts for 48 hours. Cell viability and apoptosis were detected by thiazolyl (MTT) assay and cell cycle was detected by flow cytometry (FCM). The activation of cyclin D1 (Cyclin D1), proliferating cell nuclear antigen (PCNA),) phosphatidylinositol 3 kinase (PI3K) / protein kinase B (AKT) pathway was analyzed by Western blot. Results compared with the control group (0.75 卤0.07), the BTXA (0.59 卤0.06 卤0.43 卤0.04 卤0.34 卤0.03) significantly decreased the viability of proliferative fibroblasts, and significantly increased the apoptosis rate (15.79 卤1.54), (27.32 卤2.69), (38.46 卤3.90)%, down-regulated Cyclin D1 and PCNA, and decreased the expression of PI3K and AKT phosphorylation, compared with the control group [(0.75 卤0.07), (27.32 卤2.69), (38.46 卤3.90)%], and decreased the expression of PI3K and AKT phosphorylation, compared with the control group [(0.75 卤0.07), (27.32 卤2.69), (38.46 卤3.90)%]. And cell cycle arrest in G 1 phase, the differences were statistically significant (P0.05). Conclusion BTXA can inhibit proliferation of proliferative cells by blocking PI3K/AKT signaling pathway and cyclin expression.
【作者单位】：首都医科大学附属北京朝阳医院皮肤与医疗美容科;中国医学科学院北京协和医院美容外科;
【基金】：国家自然科学基金资助项目(81071571)
【分类号】：R622

【相似文献】