创伤性轴索损伤合并低氧血症大鼠通气复苏效果的研究
发布时间：2018-09-09 17:10
【摘要】：研究目的1.在创伤性轴索损伤(TAI)合并低氧血症性二次脑损伤(SBI)大鼠模型基础上进行不同氧浓度复苏,分析比较不同氧浓度复苏之间大鼠脑组织匀浆氧自由基指标的变化规律,探讨不同氧浓度复苏对脂质过氧化的影响。2.在创伤性轴索损伤(TAI)合并低氧血症性二次脑损伤(SBI)大鼠模型基础上进行不同氧浓度复苏,分析比较不同氧浓度复苏之间大鼠脑组织病理学的变化规律,探讨不同氧浓度复苏对轴索损伤的影响。3.在创伤性轴索损伤(TAI)合并低氧血症性二次脑损伤(SBI)大鼠模型基础上进行不同氧浓度复苏,探究此模型的适宜的通气复苏策略。研究方法1.采用自制联合损伤装置制作TAI模型,经小动物呼吸机吸入低浓度氧构建低氧血症,从而形成TAI合并低氧血症的大鼠模型。2.在此模型基础上,对氧复苏后大鼠脑组织匀浆进行检测,研究不同氧浓度复苏对大鼠脂质过氧化的影响。3.在此模型基础上,对氧复苏后大鼠脑组织NF200免疫组化染色,研究不同氧浓度复苏对大鼠脑组织病理变化的影响。研究结果1.与Sham组相比,TAI+H组及各复苏组24h时MDA含量明显升高,1w后恢复至正常水平;各复苏组与TAI+H组比较,给予21%、50%氧复苏时MDA下降,差异有统计学意义(P0.05);各复苏组随氧浓度升高MDA值逐渐升高。TAI+H组及各复苏组24h时SOD含量明显下降,与Sham组比较有统计学意义(P0.05),1w时仍低于正常水平;各复苏组与TAI+H组比较,给予21%氧复苏时SOD值明显升高(P0.05),给予50%、75%氧复苏时SOD差异无统计学意义(P0.05),给予100%氧复苏时SOD值明显降低(P0.05);各复苏组随氧浓度升高SOD值逐渐降低。2.脑干部位NF200免疫组化染色显示,24h时TAI合并缺氧组的阳性染色明显强于各复苏组,21%氧浓度时的阳性染色最弱;各组阳性染色随时间而减弱,1w时各组阳性染色明显弱于24h,此时TAI合并缺氧组的阳性染色仍明显强于各复苏组,21%氧浓度时的阳性染色仍最弱。染色阳性的神经轴索半定量分析显示,24h时TAI合并缺氧组明显高于各复苏组,21%氧浓度时的值最低;各组数值随时间而降低,1w时各组值明显低于24h,此时TAI合并缺氧组的值仍高于各复苏组,21%氧浓度的值仍为最低。研究结论1、给氧治疗可以改善创伤后缺氧大鼠的低氧血症,减轻脂质过氧化导致的损伤,但不同复苏氧浓度的治疗效果差异较大。2、适当浓度给氧治疗可以显著减低轴索损伤的程度,起到神经保护作用。3、对于此TAI合并缺氧动物模型的急性期短时间氧复苏浓度不能超过50%。
[Abstract]:Objective 1. On the basis of (SBI) rat model of traumatic axonal injury (TAI) with hypoxia-induced secondary brain injury (SBI), different oxygen concentrations were resuscitated, and the changes of oxygen free radical index in brain homogenate of rats were analyzed and compared during different oxygen concentration resuscitation. To explore the effect of different oxygen concentration resuscitation on lipid peroxidation. On the basis of (SBI) rat model of traumatic axonal injury (TAI) combined with hypoxia-induced secondary brain injury (SBI), different oxygen concentration resuscitation was carried out, and the pathological changes of brain tissue were analyzed and compared between different oxygen concentration resuscitation. To investigate the effect of oxygen resuscitation on axonal damage. Different oxygen concentration resuscitation was carried out on the basis of traumatic axonal injury (TAI) combined with hypoxia-induced secondary brain injury (SBI) in rats, and the appropriate ventilation resuscitation strategy was explored. Method 1. The TAI model was made by using a self-made combined injury device. Hypoxemia was constructed by inhaling low concentration oxygen through a small animal ventilator, and then the rat model of TAI combined with hypoxemia was established. 2. On the basis of this model, the brain homogenate of rats after oxygen resuscitation was detected to study the effect of different oxygen concentration resuscitation on lipid peroxidation in rats. On the basis of this model, the effects of different oxygen concentration resuscitation on the pathological changes of rat brain tissue were studied by immunohistochemical staining of NF200 in brain tissue after oxygen resuscitation. Results 1. Compared with Sham group, MDA content in Tai H group and resuscitation group increased significantly at 24 h, and then returned to normal level at 1 week after resuscitation. Compared with TAI H group, MDA decreased during 50% oxygen resuscitation in each resuscitation group. There was significant difference between the two groups (P0.05), the SOD content in the resuscitation group gradually increased with the increase of oxygen concentration. The SOD content in the Tai H group and the resuscitation group decreased significantly at 24 hours, which was significantly lower than that in the Sham group (P0.05) at 1 week, and the SOD content in each resuscitation group was lower than that in the TAI H group at 1 week. The SOD value of 21% oxygen resuscitation was significantly higher than that of 50% 75% oxygen resuscitation (P0.05), and the SOD value of 100% oxygen resuscitation was significantly lower (P0.05). The SOD value of each resuscitation group decreased gradually with the increase of oxygen concentration. NF200 immunohistochemical staining in brain stem showed that the positive staining of TAI combined with hypoxia was significantly stronger than that of resuscitation group with 21% oxygen concentration at 24 h. The positive staining of TAI combined with hypoxia was significantly stronger than that of the resuscitation group at 21% oxygen concentration. Semi-quantitative analysis of staining positive axons showed that TAI combined with hypoxia at 24 h was significantly higher than the lowest at 21% oxygen concentration in each resuscitation group. The values of each group were significantly lower than that of the control group for 24 hours, and the value of TAI combined with hypoxia was still higher than that of the resuscitation group at 21% oxygen concentration. Conclusion 1. Oxygen administration can ameliorate hypoxemia and reduce the damage caused by lipid peroxidation in hypoxic rats after trauma. However, the therapeutic effects of different resuscitation oxygen concentrations were significantly different. The appropriate concentration of oxygen administration could significantly reduce the degree of axonal injury. Neuroprotective effect. 3. For this TAI combined with anoxic animal model, the concentration of short term oxygen resuscitation can not exceed 50%.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R651.15
本文编号：2233042
[Abstract]:Objective 1. On the basis of (SBI) rat model of traumatic axonal injury (TAI) with hypoxia-induced secondary brain injury (SBI), different oxygen concentrations were resuscitated, and the changes of oxygen free radical index in brain homogenate of rats were analyzed and compared during different oxygen concentration resuscitation. To explore the effect of different oxygen concentration resuscitation on lipid peroxidation. On the basis of (SBI) rat model of traumatic axonal injury (TAI) combined with hypoxia-induced secondary brain injury (SBI), different oxygen concentration resuscitation was carried out, and the pathological changes of brain tissue were analyzed and compared between different oxygen concentration resuscitation. To investigate the effect of oxygen resuscitation on axonal damage. Different oxygen concentration resuscitation was carried out on the basis of traumatic axonal injury (TAI) combined with hypoxia-induced secondary brain injury (SBI) in rats, and the appropriate ventilation resuscitation strategy was explored. Method 1. The TAI model was made by using a self-made combined injury device. Hypoxemia was constructed by inhaling low concentration oxygen through a small animal ventilator, and then the rat model of TAI combined with hypoxemia was established. 2. On the basis of this model, the brain homogenate of rats after oxygen resuscitation was detected to study the effect of different oxygen concentration resuscitation on lipid peroxidation in rats. On the basis of this model, the effects of different oxygen concentration resuscitation on the pathological changes of rat brain tissue were studied by immunohistochemical staining of NF200 in brain tissue after oxygen resuscitation. Results 1. Compared with Sham group, MDA content in Tai H group and resuscitation group increased significantly at 24 h, and then returned to normal level at 1 week after resuscitation. Compared with TAI H group, MDA decreased during 50% oxygen resuscitation in each resuscitation group. There was significant difference between the two groups (P0.05), the SOD content in the resuscitation group gradually increased with the increase of oxygen concentration. The SOD content in the Tai H group and the resuscitation group decreased significantly at 24 hours, which was significantly lower than that in the Sham group (P0.05) at 1 week, and the SOD content in each resuscitation group was lower than that in the TAI H group at 1 week. The SOD value of 21% oxygen resuscitation was significantly higher than that of 50% 75% oxygen resuscitation (P0.05), and the SOD value of 100% oxygen resuscitation was significantly lower (P0.05). The SOD value of each resuscitation group decreased gradually with the increase of oxygen concentration. NF200 immunohistochemical staining in brain stem showed that the positive staining of TAI combined with hypoxia was significantly stronger than that of resuscitation group with 21% oxygen concentration at 24 h. The positive staining of TAI combined with hypoxia was significantly stronger than that of the resuscitation group at 21% oxygen concentration. Semi-quantitative analysis of staining positive axons showed that TAI combined with hypoxia at 24 h was significantly higher than the lowest at 21% oxygen concentration in each resuscitation group. The values of each group were significantly lower than that of the control group for 24 hours, and the value of TAI combined with hypoxia was still higher than that of the resuscitation group at 21% oxygen concentration. Conclusion 1. Oxygen administration can ameliorate hypoxemia and reduce the damage caused by lipid peroxidation in hypoxic rats after trauma. However, the therapeutic effects of different resuscitation oxygen concentrations were significantly different. The appropriate concentration of oxygen administration could significantly reduce the degree of axonal injury. Neuroprotective effect. 3. For this TAI combined with anoxic animal model, the concentration of short term oxygen resuscitation can not exceed 50%.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R651.15
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