氢气饱和等渗盐水腹腔注射治疗兔创伤性关节炎的实验研究
发布时间：2018-10-08 18:11
【摘要】：目的:氢气具有抗炎症、抗氧化作用,但对于其治疗创伤性关节炎(traumatic arthritis,TA)的效果鲜有报道。文中旨在研究氢气饱和等渗盐水腹腔注射对兔创伤性关节炎的治疗效果。方法:采用随机数字表法将26只新西兰家兔分为正常对照组(n=6)、模型实验组(n=10)和模型对照组(n=10)。模型实验组腹腔注射氢气饱和等渗盐水8mL/kg,每3天1次,模型对照组腹腔注射等量等渗盐水,正常对照组不做任何处理,治疗持续6周。于治疗前后分别取3组家兔血清及关节液测定一氧化氮(nitric oxide,NO)和透明质酸(hyaluronic acid,HA)的含量,结合家兔的行为学表现对治疗效果进行评价。结果:模型实验组治疗后行为学有明显改善(P0.05)。膝关节肿胀评分治疗前各组差异无统计学意义(P0.05);治疗后模型实验组优于模型对照组(P0.05))。治疗前2个模型组血清及关节液中NO含量升高,差异有统计学意义(P0.05),治疗后模型实验组明显下降,其中血清中NO含量由(79.58±13.46)降至(76.23±12.15),关节液中NO含量由(89.45±14.98)降至(80.36±12.78),差异有统计学意义(P0.05)。治疗后模型实验组血清中HA含量明显下降[(436.82±60.91)vs(340.21±40.57),P0.05],关节液中显著上升[(1.72±0.37)vs(2.47±0.62),P0.05]。结论:腹腔注射氢气饱和等渗盐水在治疗兔创伤性关节炎方面具有一定的疗效。
[Abstract]:Objective: hydrogen has anti-inflammatory and anti-oxidation effects, but its effect on traumatic arthritis (traumatic arthritis,TA) is rarely reported. The purpose of this paper is to study the effect of intraperitoneal injection of hydrogen saturated isosmotic saline on traumatic arthritis in rabbits. Methods: 26 New Zealand rabbits were randomly divided into three groups: normal control group (n = 6), model group (n = 10) and model control group (n = 10). The model group was injected with 8 mL / kg hydrogen saturated isoosmotic saline once every 3 days. The model control group was injected with the same amount of isosmotic saline, while the normal control group was treated for 6 weeks without any treatment. The contents of nitric oxide (nitric oxide,NO) and hyaluronic acid (hyaluronic acid,HA) were measured in serum and articular fluid of three groups before and after treatment. Results: the behavior of the model group was significantly improved after treatment (P0.05). There was no significant difference in knee swelling score before treatment (P0.05), and the model group was superior to the model control group (P0.05).) after treatment. Before treatment, the NO content in serum and joint fluid of the two model groups increased significantly (P0.05). After treatment, the NO content in the model group decreased from (79.58 卤13.46) to (76.23 卤12.15), and the NO content in the articular fluid decreased from (89.45 卤14.98) to (80.36 卤12.78) (P0.05). After treatment, the serum HA content in the model group was significantly decreased [(436.82 卤60.91) vs (卤40.57) P 0.05], and the joint fluid was significantly increased [(1.72 卤0.37) vs (2.47 卤0.62) P 0.05]. Conclusion: intraperitoneal injection of hydrogen saturated isosmotic saline is effective in the treatment of traumatic arthritis in rabbits.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R684.3
本文编号：2257768
[Abstract]:Objective: hydrogen has anti-inflammatory and anti-oxidation effects, but its effect on traumatic arthritis (traumatic arthritis,TA) is rarely reported. The purpose of this paper is to study the effect of intraperitoneal injection of hydrogen saturated isosmotic saline on traumatic arthritis in rabbits. Methods: 26 New Zealand rabbits were randomly divided into three groups: normal control group (n = 6), model group (n = 10) and model control group (n = 10). The model group was injected with 8 mL / kg hydrogen saturated isoosmotic saline once every 3 days. The model control group was injected with the same amount of isosmotic saline, while the normal control group was treated for 6 weeks without any treatment. The contents of nitric oxide (nitric oxide,NO) and hyaluronic acid (hyaluronic acid,HA) were measured in serum and articular fluid of three groups before and after treatment. Results: the behavior of the model group was significantly improved after treatment (P0.05). There was no significant difference in knee swelling score before treatment (P0.05), and the model group was superior to the model control group (P0.05).) after treatment. Before treatment, the NO content in serum and joint fluid of the two model groups increased significantly (P0.05). After treatment, the NO content in the model group decreased from (79.58 卤13.46) to (76.23 卤12.15), and the NO content in the articular fluid decreased from (89.45 卤14.98) to (80.36 卤12.78) (P0.05). After treatment, the serum HA content in the model group was significantly decreased [(436.82 卤60.91) vs (卤40.57) P 0.05], and the joint fluid was significantly increased [(1.72 卤0.37) vs (2.47 卤0.62) P 0.05]. Conclusion: intraperitoneal injection of hydrogen saturated isosmotic saline is effective in the treatment of traumatic arthritis in rabbits.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R684.3
【参考文献】
相关期刊论文 前1条
1 林曦;高劲谋;韦功滨;;氨基葡萄糖治疗创伤性膝关节炎32例[J];中国药业;2008年05期
，本文编号：2257768
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