ATP敏感性钾通道在羟考酮后处理减轻大鼠心肌缺血再灌注损伤中的作用
发布时间：2018-10-08 20:30
【摘要】：目的:评价ATP敏感性钾通道(KATP通道)在羟考酮后处理减轻大鼠心肌缺血再灌注损伤中的作用。方法:成年健康雄性SD大鼠50只,8~12周龄,体重200~300 g,采用随机数字表法分为5组(n=10):假手术组(S组)、缺血再灌注组(I/R组)、羟考酮后处理组(OP组)、羟考酮联合κ受体阻断剂nor-binaltorphimine(Nor-BNI)组(ON组)和KATP通道阻断剂格列苯脲(Glibenclamide)+羟考酮后处理组(GOP组)。采用结扎冠状动脉前降支30 min,再灌注120 min的方法制备大鼠心肌缺血再灌注损伤模型,S组只穿线,不结扎左冠状动脉前降支。ON组与GOP组分别于缺血前颈静脉注射Nor-BNI 2mg/kg和格列苯脲1mg/kg;OP组、ON组与GOP组于再灌注前5min时颈静脉注射羟考酮0.5mg/kg,S组和I/R组给予等容量生理盐水。各组于再灌注120 min时采集颈动脉血样,检测血清肌钙蛋白I(cTnI)和乳酸脱氢酶(LDH)的浓度。然后处死大鼠,取左心室,确定心肌梗死体积。结果:与S组比较,I/R组、OP组、ON组和GOP组血清中cTnI浓度、LDH浓度和心肌梗死体积升高(P0.05);与I/R组比较,OP组、ON组和GOP组血清c TnI浓度、LDH浓度和心肌梗死体积降低(P0.05);与OP组比较,ON组和GOP组血清cTnl浓度、LDH浓度和心肌梗死体积升高(P0.05)。结论:KATP通道的开放参与了羟考酮后处理减轻大鼠心肌缺血再灌注损伤。
[Abstract]:Aim: to evaluate the role of ATP sensitive potassium channel (KATP channel) in relieving myocardial ischemia reperfusion injury in rats after treatment with hydroxycodone. Methods: 50 healthy male SD rats were 12 weeks old. Weighing 200,300 g, the rats were randomly divided into five groups (n = 10): sham operation group (S group), ischemia reperfusion group (I / R group), hydroxycodone post-treatment group (OP group), hydroxycodone plus 魏 receptor blocker nor-binaltorphimine (Nor-BNI) group (ON group) and KATP channel blocker glibenclamide (KATP channel blocker). (Glibenclamide) hydroxycodone group (GOP group). Rat myocardial ischemia reperfusion injury model was established by ligating anterior descending coronary artery for 30 min, and reperfusion for 120 min. No ligation of left anterior descending coronary artery. On group and GOP group were injected with Nor-BNI 2mg/kg and glibenclamide 1 mg / kg op group before ischemia. On group and GOP group were given equal volume saline in jugular vein injection of hydroxycodone 0.5 mg / kg / kg S and I / R group respectively during 5min before reperfusion. Carotid blood samples were collected at 120 min after reperfusion. Serum troponin I (cTnI) and lactate dehydrogenase (LDH) were measured. Then the rats were killed and the left ventricle was taken to determine the volume of myocardial infarction. Results: compared with S group, serum cTnI concentration and myocardial infarction volume in op group and GOP group were increased (P0.05), c TnI concentration and myocardial infarction volume were decreased in op group (P0.05) and in GOP group (P0.05). Serum cTnl concentration and myocardial infarction volume were higher than those in on group and GOP group (P 0.05). Conclusion the opening of the K-ATP channel may play an important role in reducing myocardial ischemia reperfusion injury after treatment with hydroxycodone in rats.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R614
[Abstract]:Aim: to evaluate the role of ATP sensitive potassium channel (KATP channel) in relieving myocardial ischemia reperfusion injury in rats after treatment with hydroxycodone. Methods: 50 healthy male SD rats were 12 weeks old. Weighing 200,300 g, the rats were randomly divided into five groups (n = 10): sham operation group (S group), ischemia reperfusion group (I / R group), hydroxycodone post-treatment group (OP group), hydroxycodone plus 魏 receptor blocker nor-binaltorphimine (Nor-BNI) group (ON group) and KATP channel blocker glibenclamide (KATP channel blocker). (Glibenclamide) hydroxycodone group (GOP group). Rat myocardial ischemia reperfusion injury model was established by ligating anterior descending coronary artery for 30 min, and reperfusion for 120 min. No ligation of left anterior descending coronary artery. On group and GOP group were injected with Nor-BNI 2mg/kg and glibenclamide 1 mg / kg op group before ischemia. On group and GOP group were given equal volume saline in jugular vein injection of hydroxycodone 0.5 mg / kg / kg S and I / R group respectively during 5min before reperfusion. Carotid blood samples were collected at 120 min after reperfusion. Serum troponin I (cTnI) and lactate dehydrogenase (LDH) were measured. Then the rats were killed and the left ventricle was taken to determine the volume of myocardial infarction. Results: compared with S group, serum cTnI concentration and myocardial infarction volume in op group and GOP group were increased (P0.05), c TnI concentration and myocardial infarction volume were decreased in op group (P0.05) and in GOP group (P0.05). Serum cTnl concentration and myocardial infarction volume were higher than those in on group and GOP group (P 0.05). Conclusion the opening of the K-ATP channel may play an important role in reducing myocardial ischemia reperfusion injury after treatment with hydroxycodone in rats.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R614
【参考文献】
相关期刊论文 前7条
1 杨s钅，
本文编号：2258161
本文链接：https://www.wllwen.com/yixuelunwen/waikelunwen/2258161.html
最近更新
教材专著
