他克莫司下调实验性自身免疫性肌炎小鼠肌组织中IL-17和IL-23 mRNA水平

发布时间：2018-10-17 20:57

【摘要】：目的研究白细胞介素17(IL-17)、IL-23 mRNA在实验性自身免疫性肌炎(EAM)小鼠肌组织表达情况,探讨他克莫司(TAC)处理对IL-17、IL-23 mRNA水平的影响及对EAM小鼠的治疗效果。方法 15只雌性BALB/c小鼠随机分为正常对照组、EAM模型组和TAC处理组;HE染色观察肌组织炎症程度;实时荧光定量PCR检测小鼠肌组织IL-17、IL-23 mRNA水平;Pearson法分析二者间的相关性。结果与正常组相比,EAM组小鼠肌组织IL-17、IL-23 mRNA水平显著升高,给予TAC处理后均明显降低。二者表达水平与肌组织炎症评分呈正相关。与EAM组比较,TAC处理组小鼠肌组织炎症评分也有所下降。结论 TAC可下调EAM小鼠IL-17、IL-23 mRNA水平。
[Abstract]:Objective to study the expression of interleukin 17 (IL-17) and IL-23 mRNA in the muscle tissue of (EAM) mice with experimental autoimmune myositis and to explore the effect of tacrolimus (TAC) treatment on IL-17,IL-23 mRNA level and on EAM mice. Methods Fifteen female BALB/c mice were randomly divided into three groups: normal control group, EAM model group and TAC treated group. The inflammation degree of muscle tissue was observed by HE staining, the IL-17,IL-23 mRNA level of muscle tissue was detected by real-time fluorescence quantitative PCR, and the correlation between them was analyzed by Pearson method. Results compared with the normal group, the level of IL-17,IL-23 mRNA in the EAM group was significantly higher than that in the normal group, and decreased significantly after TAC treatment. There was a positive correlation between the expression level and the inflammatory score of muscle tissue. Compared with EAM group, the inflammatory score of muscle tissue in TAC treated mice was also decreased. Conclusion TAC can down-regulate the level of IL-17,IL-23 mRNA in EAM mice.
【作者单位】： 第四军医大学西京医院神经内科;西安市儿童医院;南方医科大学深圳医院;
【基金】：国家自然科学基金(81171181)
【分类号】：R685.2
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本文编号：2277953