视黄醇结合蛋白-4在Roux-en-Y胃旁路术治疗2型糖尿病中的作用研究

发布时间：2018-10-19 14:47

【摘要】：目的：2型糖尿病大鼠行Ronx-en-Y胃旁路术(Ronx-en-Y gastric bypass, RYGB),测定术后短期内(8周)血清中视黄醇结合蛋白-4(Retinol binding protein-4, RBP-4)水平的变化,为手术治疗2型糖尿病提供理论基础及发现潜在可能的机制。方法：首先参照课题组前期研究完成2型糖尿病大鼠模型(SD雄性)的建立,然后取20只已成模的大鼠采用完全随机分组方法分为2组：RYGB手术组(n=10)、假手术组(n=10)。在术前及术后1、2、4、8周,血糖仪检测血糖,采取大鼠眶静脉血,使用酶联免疫吸附测定法(Enzyme-linked immunosorbent assay, ELISA)试剂盒测定血清中视RBP-4含量,术后8周处死大鼠,取大鼠双下肢的腓肠肌,使用ELISA试剂盒测定肌肉中的葡萄糖转运蛋-4(GLUT-4)含量。结果：术后手术组死亡3只,假手术组无死亡；2型糖尿病大鼠在行RYGB手术后,手术组较假手术组在大鼠体重、血糖值明显下降；手术组与假手术组相比,大鼠肌肉中的GLUT4含量上升明显；而RBP-4在手术组比假手术组显著降低,且与肌肉中的葡萄糖转运蛋-4量成负相关。结论：1.RYGB可以使2型糖尿病大鼠体重及血糖下降,且血糖下降维持的时间更长,说明术后空腹血糖的下降不仅仅与体重减轻有关；2.2型糖尿病大鼠血清中的RBP-4含量水平较高而骨骼肌中的GLUT-4含量较低,行RYGB手术后RBP-4明显下降,而骨骼肌中的GLUT-4明显升高,且RBP-4与GLUT-4呈负相关。3.我们推测脂肪因子RBP-4在手术治疗2型糖尿病机制可能是：RYGB使2型糖尿病大鼠血清中RBP-4水平下降,其增加肌肉组织中的GLUT-4含量,从而增加肌肉对葡萄糖的摄入,从而降低血糖水平。
[Abstract]:Objective: to study the changes of retinol binding protein-4 (Retinol binding protein-4, RBP-4 in serum of type 2 diabetic rats by Ronx-en-Y gastric bypass (Ronx-en-Y gastric bypass, RYGB),) in a short period after operation (8 weeks), so as to provide a theoretical basis for the surgical treatment of type 2 diabetes and to find out the possible mechanism. Methods: first, the type 2 diabetic rat model (SD male) was established according to the previous study of the research group, and then 20 rats were divided into two groups randomly: RYGB operation group (n = 10), sham operation group (n = 10) and sham operation group (n = 10), and then 20 rats were randomly divided into two groups: RYGB operation group (n = 10) and sham operation group (n = 10). Blood glucose was detected by blood glucose analyzer before operation and 8 weeks after operation. The serum RBP-4 content was measured by Enzyme-linked immunosorbent assay, ELISA) kit. The gastrocnemius muscle of both lower extremities of the rats were killed 8 weeks after the operation, and the gastrocnemius muscles of the lower extremities of the rats were taken out, and the serum RBP-4 content was determined by using the enzyme linked immunosorbent assay (Enzyme-linked immunosorbent assay, ELISA) kit. The content of glucose transport egg-4 (GLUT-4) in muscle was determined by ELISA kit. Results: three rats died in the operation group, but no death in the sham operation group. After RYGB operation, the body weight and blood sugar value of the operation group were significantly lower than those of the sham operation group, and compared with the sham operation group, the blood sugar level of the operation group was significantly lower than that of the sham operation group. The content of GLUT4 increased significantly in the muscle of rats, but RBP-4 in the operation group was significantly lower than that in the sham operation group, and was negatively correlated with the amount of glucose transport egg 4 in the muscle. Conclusion: 1.RYGB can decrease the body weight and blood sugar of type 2 diabetic rats, and the decrease of blood sugar will last longer, indicating that the decrease of fasting blood glucose after operation is not only related to weight loss. The level of RBP-4 in serum of type 2 2 diabetic rats was higher than that in skeletal muscle. The content of RBP-4 in skeletal muscle decreased significantly after RYGB operation, while GLUT-4 in skeletal muscle increased significantly, and RBP-4 was negatively correlated with GLUT-4. We speculate that the mechanism of RBP-4 in the surgical treatment of type 2 diabetes may be that RYGB can decrease the level of RBP-4 in the serum of type 2 diabetic rats, and increase the content of GLUT-4 in muscle tissue, thereby increasing the intake of glucose by the muscle. In order to lower the level of blood sugar.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.1;R656.6

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