持续加力和间歇加力对骨髓基质干细胞增殖和分化的影响

发布时间：2018-10-20 06:49

【摘要】：背景:临床运用牵张成骨技术时常用的加力方式有2种:持续加力和间歇加力。但是,哪种方式更有利于新骨形成尚有争议。目的:观察持续加力和间歇加力对骨髓基质干细胞分化及增殖能力的影响。方法:密度梯度离心法分离培养兔骨髓基质干细胞,传至3代接种至6孔细胞培养板进行力学实验。空白组不作离心处理,静态培养;持续加力组每天加载离心4 h(1 100 r/min,相对离心力20×g);间歇加力组每次加载离心力24 min(1 100 r/min,相对离心力100×g),每天4 h内2次,2 h加力1次。力学刺激3 d停止培养,检测细胞增殖活性、碱性磷酸酶活性、骨钙素水平。结果与结论:(1)与空白组比较,机械离心力能促进骨髓基质干细胞成骨分化和增殖,增强碱性磷酸酶活性(P0.05);(2)持续加力较间歇加力对骨髓基质干细胞分化增殖及碱性磷酸酶活性影响更显著(P0.05)。
[Abstract]:Background: there are two kinds of exertion methods in clinical application of distraction osteogenesis: continuous and intermittent stress. However, the way in which new bone formation is more conducive is controversial. Aim: to observe the effects of continuous and intermittent stress on the differentiation and proliferation of bone marrow stromal cells. Methods: rabbit bone marrow stromal cells were isolated and cultured by density gradient centrifugation. The blank group was not treated by centrifugation and cultured statically, the continuous adding group was subjected to centrifugation for 4 h (1 100r / min) daily, the relative centrifugal force (20 脳 g);) was applied for 24 min (1 100r / min), the relative centrifugal force (100 脳 g),) was applied twice a day for 4 h and once for 2 h. The cell proliferation activity, alkaline phosphatase activity and osteocalcin level were measured after mechanical stimulation for 3 days. Results and conclusion: (1) compared with the blank group, mechanical centrifugal force can promote osteogenic differentiation and proliferation of bone marrow stromal cells. The enhancement of alkaline phosphatase activity (P0.05); (2) had more significant effect on differentiation and proliferation of bone marrow stromal cells than intermittent stress (P0.05).
【作者单位】：西南医科大学附属口腔医院口腔颌面外科;西南医科大学口颌面修复重建和再生实验室;
【基金】：国家自然科学基金项目(81371125) 西南医科大学附属口腔医院课题(20130412)~~
【分类号】：R68

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