人胎盘提取物对成骨细胞增殖、分化、矿化及骨愈合的实验研究

发布时间：2018-10-24 11:07

【摘要】：目的研究人胎盘提取物(human placenta extract,HPE)对体外成骨细胞的增殖、分化、矿化的影响,进一步了解HPE促进骨折愈合的机理。方法用成骨细胞系(h Fob1.19细胞),通过CCK-8、流式细胞术、Real-Time PCR法分别检测不同剂量的HPE(0、18μl、100μl、180μl、360μl)在不同时间对成骨细胞增殖的影响;通过检测碱性磷酸酶(alkaline phosphatase,ALP)观察不同剂量的HPE和Rhbmp-2(0、18μl HPE、100μl HPE、100μl Rhbmp-2、100μl HPE+100μl Rhbmp-2)在不同时间对成骨细胞分化的影响;通过茜素红染色检测不同剂量的HPE(0、50μl、100μl、200μl)在不同时间对成骨细胞矿化的影响,系统研究体外HPE对成骨细胞增殖、分化、矿化的影响及其促进骨愈合的可能机制。结果空白对照组和加入不同剂量的HPE各组对成骨细胞的增殖、分化未观察到明显的影响(P0.05),而加入不同剂量的HPE(50μl、100μl、200μl)各组对成骨细胞的矿化比空白对照组有明显促进作用(P0.01)。结论体外HPE对成骨细胞的增殖、分化无明显影响,对成骨细胞的矿化有明显促进作用;HPE促进成骨细胞矿化可能是促进骨折愈合机制。
[Abstract]:Objective to study the effects of human placental extract (human placenta extract,HPE) on the proliferation, differentiation and mineralization of osteoblasts in vitro, and to understand the mechanism of HPE promoting fracture healing. Methods Osteoblast cell line (h Fob1.19 cell) was used to detect the effects of different doses of HPE (0 18 渭 L ~ 100 渭 l ~ (100 渭 l) ~ 180 渭 L ~ (360 渭 l) on the proliferation of osteoblasts at different time by CCK-8, flow cytometry and Real-Time PCR method. The effects of different doses of HPE and Rhbmp-2 (0 18 渭 l HPE,100 渭 l Rhbmp-2100 渭 l HPE 100 渭 l Rhbmp-2) on the differentiation of osteoblasts at different time were observed by detecting alkaline phosphatase (alkaline phosphatase,ALP), and the mineralization of osteoblasts was detected by alizarin red staining. The effects of HPE on the proliferation, differentiation and mineralization of osteoblasts in vitro and the possible mechanism of promoting bone healing were studied. Results the proliferation and differentiation of osteoblasts in the blank control group and the different dosage HPE groups were not significantly affected (P0.05), but the mineralization of osteoblasts was significantly promoted by adding different doses of HPE (50 渭 l-1 100 渭 l / 200 渭 l) (P0.01). Conclusion HPE has no effect on the proliferation and differentiation of osteoblasts in vitro, but it can promote the mineralization of osteoblasts, and the mechanism of promoting the mineralization of osteoblasts by HPE may be the mechanism of promoting fracture healing.
【作者单位】：贵州医科大学;贵州医科大学附属医院;
【基金】：国家自然科学基金项目(81560266) 贵州医科大学附属医院博士启动基金
【分类号】：R683

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