玻璃酸钠预防移植静脉术后粘连的实验研究

发布时间：2018-10-25 06:54

【摘要】：目的探讨局部应用玻璃酸钠对移植静脉术后粘连的影响。方法健康雄性5月龄新西兰大白兔36只,随机分为A组(透明质酸钠组)、B组(手术对照组),每组18只。切取右侧颈外静脉3cm,游离左侧颈总动脉,中间切除长约1cm,建立动脉缺损模型,将切取静脉倒置后移植于动脉缺损处,吻合结束后A组用透明质酸钠凝胶0.2ml涂布于移植静脉外膜及两个吻合口,B组应用生理盐水作对照。于术后1、2、4周行移植静脉取材,切取前行大体观察,切取移植静脉后行HE染色、Masson染色观察移植静脉周围粘连和管壁组织学变化,并对术后2、4周的移植静脉粘连行大体观察分级和组织学分级。结果术后1、2、4周大体观察及组织学观察A组粘连程度均低于B组,术后2、4周A组移植静脉大体粘连分级和组织学分级均低于B组,差异有统计学意义(P0.05)。结论局部应用透明质酸钠可有效抑制移植静脉术后粘连。
[Abstract]:Objective to investigate the effect of local application of sodium hyaluronate on postoperative adhesion of vein grafts. Methods Thirty-six healthy 5-month-old New Zealand white rabbits were randomly divided into group A (sodium hyaluronate group,), B group) and operation control group (18 rabbits in each group). The right external jugular vein was removed 3 cm, the left common carotid artery was dissociated, the length of the artery defect was about 1 cm, and the artery defect model was established. After anastomosis, group A was coated with sodium hyaluronate gel 0.2ml on the epigastric graft and two anastomotic stoma, group B was treated with normal saline as control. The grafts were taken at 1 and 2 weeks postoperatively. The grafts were observed before and after removal. HE staining and Masson staining were used to observe the adhesions around the grafts and the histologic changes of the wall of the grafts. Gross and histological grades were observed 2 weeks after operation. Results the degree of adhesion in group A was lower than that in group B at 4 weeks after operation, and the grade of gross adhesion and histology in group A was lower than that in group B at 24 weeks after operation (P0.05). Conclusion Local application of sodium hyaluronate can effectively inhibit postoperative adhesion of vein grafts.
【作者单位】：解放军260医院骨科;
【分类号】：R654.4

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