消化人胰腺以获取胰岛细胞的技术研究
发布时间：2018-10-25 19:50
【摘要】：目的： 目前胰岛移植在国际上已经逐渐从实验室技术转化为临床治疗手段,并取得了令人满意的效果。本实验通过消化心脏死亡供体胰腺器官获取足量质优的胰岛细胞团以用于胰岛纯化,进而实现临床胰岛移植,达到治愈糖尿病的目的。 实验方法： 通过专业的器官获取团队获取心脏死亡供体胰腺,保存于4℃器官保存液(UW液或HTK液)中；送达实验室后,由专业团队修剪胰腺,去除胰周组织,暴露胰管；胰管高压灌注胰腺消化液10分钟；基于胶原酶以半自动人胰腺分离装置消化胰腺组织,获取胰岛细胞团；通过DTZ染色后进行胰岛计数,并评估胰岛纯度,以用于进一步的胰岛纯化、培养和功能鉴定。 结果： 通过器官获取团队获取10条优质胰腺,胰腺平均质量为56.9±24.1g,10条胰腺灌注效果良好,对10条胰腺的消化,平均获取712068±459623个胰岛,平均胰岛当量为635458±510168IEQ,以用于胰岛的纯化。 结论： 通过实验建立了一套可靠地胰腺消化系统,获取了胰岛分离的成功,为下一步的胰岛纯化、功能鉴定,以及更进一步的胰岛移植奠定了坚实的基础。
[Abstract]:Objective: at present, islet transplantation has been gradually transformed from laboratory technique to clinical treatment in the world, and satisfactory results have been obtained. In this experiment, sufficient and high quality islet cell clusters were obtained by digesting the pancreatic organs of cardiac death donor for islet purification, and then clinical islet transplantation was achieved, and the goal of curing diabetes was achieved. Methods: the pancreas of cardiac death donor was obtained by professional organ acquisition team and stored in 4 鈩，
本文编号：2294660
[Abstract]:Objective: at present, islet transplantation has been gradually transformed from laboratory technique to clinical treatment in the world, and satisfactory results have been obtained. In this experiment, sufficient and high quality islet cell clusters were obtained by digesting the pancreatic organs of cardiac death donor for islet purification, and then clinical islet transplantation was achieved, and the goal of curing diabetes was achieved. Methods: the pancreas of cardiac death donor was obtained by professional organ acquisition team and stored in 4 鈩，
本文编号：2294660
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