丙泊酚对发育小鼠海马齿状回神经干细胞的影响

发布时间：2018-10-30 07:48

【摘要】：目的观察丙泊酚对发育小鼠海马齿状回神经干细胞的影响。方法将健康同窝日龄7d(P7)的C57小鼠分为3组:高剂量丙泊酚组、低剂量丙泊酚组和10%脂肪乳对照组。所有小鼠P7接受药物处理。高剂量丙泊酚组腹腔注射丙泊酚60mg/kg;低剂量丙泊酚组腹腔注射丙泊酚30mg/kg;对照组腹腔注射同等体积的10%脂肪乳。部分小鼠注药24h(P8)后处死,其余小鼠饲养至日龄14d(P14)处死。采用免疫组织化学法检测海马齿状回中Ki67、巢蛋白(Nestin)、脑脂结合蛋白(BLBP)及神经元核心抗原(NeuN)的形态及表达量。结果高剂量丙泊酚组P8小鼠海马齿状回中Ki67标记的神经干细胞数量较10%脂肪乳对照组明显减少(P0.01),且Nestin及BLBP标记的干细胞纤维数量、长度均受损(P0.05)。P14小鼠海马齿状回中NeuN阳性细胞数量低剂量丙泊酚组与10%脂肪乳对照组比较差异无统计学意义(P0.05),而高剂量丙泊酚组较10%脂肪乳对照组显著减少(P0.01)。结论高剂量丙泊酚可抑制海马齿状回神经干细胞的增殖,损伤神经干细胞突起数量及突起形态,导致神经元成熟障碍。
[Abstract]:Objective to observe the effect of propofol on neural stem cells in dentate gyrus of developing mice. Methods C57 mice were divided into three groups: high dose propofol group, low dose propofol group and 10% fat milk control group. All mice were treated with P7. Propofol 60 mg / kg was injected intraperitoneally in high dose propofol group, 30 mg / kg propofol was injected intraperitoneally in low dose propofol group and 10% fat milk was injected intraperitoneally in control group. Some mice were killed after 24 h (P8) administration, and others were killed at 14 days of age (P14). The morphology and expression of Ki67, nestin (Nestin), brain lipid binding protein (BLBP) and neuron core antigen (NeuN) in dentate gyrus were detected by immunohistochemical method. Results the number of Ki67 labeled neural stem cells in dentate gyrus of P8 mice in high dose propofol group was significantly lower than that in 10% fat milk group (P0.01), and the number of Nestin and BLBP labeled stem cell fibers was significantly lower than that of 10% fat milk control group (P0.01). The number of NeuN positive cells in dentate gyrus of P14 mice was not significantly different from that of 10% fat milk control group (P0.05), and the number of NeuN positive cells in low dose propofol group was not significantly different from that in 10% fat milk control group (P0.05). The high dose propofol group was significantly lower than the 10% fat milk control group (P0.01). Conclusion High dose propofol can inhibit the proliferation of neural stem cells in dentate gyrus, damage the number and shape of neural stem cell processes, and lead to neuronal maturation disorder.
【作者单位】：第三军医大学新桥医院麻醉科;
【基金】：国家自然科学基金资助项目(81370210)
【分类号】：R614

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