胃转流手术对糖尿病大鼠肝脏糖代谢相关因子表达的影响

发布时间：2018-10-30 18:44

【摘要】：目的:通过建立肥胖性2型糖尿病(T 2 D M)胃转流手术(G B P)大鼠模型,探讨G B P手术对肝脏糖代谢相关因子表达的影响。方法:将1 2只6周龄S D大鼠随机分为正常对照组(N C D,n=4)、高脂组(H F D,n=4)和高脂手术组(H F D-G B P,n=4)。H F D组大鼠经高脂饲料喂养4周后,通过腹腔注射链脲佐菌素构建T 2 D M模型,术前比较两组体质量、空腹血糖(F P G)、葡萄糖耐受、胰岛素敏感性的变化。2周后,构建H F D-G B P组胃转流手术模型,连续观察2周,比较手术前后两组体质量、空腹血糖、血清胆汁酸(B A)、总胆固醇(T C H)、甘油三酯(T G)的变化,并分别提取3组大鼠肝组织的总R N A和蛋白,通过R T-P C R、W e s t e r n印迹法检测法呢醇X受体(F X R)、磷酸烯醇式丙酮酸羧基激酶(P E P C K)、葡萄糖-6-磷酸酶(G 6 P a s e)、葡萄糖转运体2(G L U T 2)和成纤维细胞生长因子-2 1(F G F-2 1)的表达水平。结果:与N C D和H F D组大鼠相比,H F D-G B P大鼠血清总胆汁酸水平显著升高(p0.0 5;p0.0 1);与N C D组相比较,H F D组的血清总胆固醇和甘油三酯水平明显升高(p0.0 5;p0.0 5);与H F D组相比,H F D-G B P组大鼠体重下降,血糖含量降低(p0.0 5),血清总胆固醇和甘油三酯水平显著降低(p0.0 5;p0.0 1),术后肝脏F X R和G L U T 2 m R N A表达明显上调(p0.0 5),P E P C K、G 6 P a s e和F G F-2 1 m R N A水平表达也增加;与N C D组相比,H F D和H F D-G B P两组F X R和P E P C K蛋白表达明显升高(p0.0 5)。结论:在肥胖型T 2 D M大鼠模型中,G B P手术能有效改善糖代谢;G B P术后的血清胆汁酸水平明显升高;F X R、P E P C K、G L U T 2和F G F-2 1的表达变化可能参与G B P术后的血糖调节过程。
[Abstract]:Objective: to investigate the effect of G B P operation on the expression of hepatic glucose metabolism related factors in obese type 2 diabetes mellitus (T 2 D M) gastric bypass) (G B P) rat model. Methods: twelve 6-week-old SD rats were randomly divided into three groups: normal control group (N C), hyperlipidemic group (H F) and high-fat operation group (H F D-G B PnN 4). H F D group) fed with high fat diet for 4 weeks. The T 2D M model was established by intraperitoneal injection of streptozotocin. The changes of body mass, fasting glucose (F P G), tolerance and insulin sensitivity were compared between the two groups before and after 2 weeks. A gastric bypass model was established in group H F D-G B P and observed for 2 weeks. The changes of body mass, fasting blood glucose and serum bile acid (B A), total cholesterol (T C H), triglyceride (T G) were compared between the two groups before and after operation. The total R N A and protein were extracted from the liver tissues of three groups of rats, and the (P E P C K), of (F X R), phosphoenolpyruvate carboxykinase was detected by RT-P C RW e s t e r n blot. The expression of glucose-6-phosphatase (G6 P a s e), glucose transporter 2 (G L U T 2) and fibroblast growth factor-21 (F G F-21 was detected. Results: compared with N C D and H F D groups, the serum total bile acid level of, H F D-G B P rats was significantly increased (p 0.05 P 0.01). Compared with the N C D group, the serum total cholesterol and triglyceride levels in the, H F D group were significantly higher than those in the, H F D group (p0.05, p0.05). Compared with H F D group, H F D-G B P group decreased body weight, blood glucose content (p0.05), serum total cholesterol and triglyceride levels (p0.05). The expression of F X R and G L U T 2 m R N A was up-regulated (p0.05), P E P C KnG 6 P a s e and F G F-21 m R N A). Compared with N C D group, F X R and P E P C K protein expression in, H F D and H F D G B P groups were significantly increased (p 0.05). Conclusion: in obese T 2D M rat model, G B P operation can effectively improve the serum bile acid level after glucose metabolism; G B P. The changes of the expression of F X RN P E P C KG L U T 2 and F G F 21 may be involved in the regulation of blood glucose after G B P.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.1;R656.61

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