髓核组织高温相关丝氨酸蛋白酶A1表达水平与椎间盘退变程度的相关性

发布时间：2018-11-01 10:41

【摘要】：目的分析髓核组织高温相关丝氨酸蛋白酶A1(HtrA1)的表达水平与椎间盘退变程度(Pfirrmann分级)的相关性。方法 36例需手术摘除髓核组织的患者,术前行磁共振成像检查,据矢状位T2加权相磁共振对拟手术节段予Pfirrmann分级;采用逆转录PCR及Western blot方法检测髓核组织中HtrA1的mRNA及蛋白表达水平,对髓核组织HtrA1的表达水平与Pfirrmann等级进行相关性分析。结果 36例椎间盘经磁共振成像检查,PfirrmannⅠ级3例、Ⅱ级10例、Ⅲ级11例、Ⅳ级7例、Ⅴ级5例;髓核组织HtrA1 mRNA及蛋白表达水平随Pfirrmann等级增高而增加,经Bonferroni检验,除Ⅳ、Ⅴ级间外(P0.05),余各级间两两比较差异均有统计学意义(P0.05),经Spearman秩相关分析:HtrA1 mRNA及蛋白表达水平与Pfirrmann分级间存在秩相关关系(P0.000 1)。结论髓核组织HtrA1 mRNA及蛋白表达水平随Pfirrmann等级增高而升高,提示HtrA1与椎间盘退变程度存在相关性。
[Abstract]:Objective to analyze the correlation between the expression of hyperthermia associated serine protease A1 (HtrA1) and the degree of disc degeneration (Pfirrmann grade) in nucleus pulposus. Methods Thirty-six patients with nucleus pulposus were surgically removed. MRI was performed before operation. Pfirrmann was graded according to sagittal T 2-weighted magnetic resonance imaging. The expression of mRNA and protein of HtrA1 in nucleus pulposus was detected by reverse transcription PCR and Western blot, and the correlation between the expression of HtrA1 and Pfirrmann grade in nucleus pulposus was analyzed. Results 36 cases of intervertebral disc were examined by magnetic resonance imaging. There were 3 cases of Pfirrmann grade 鈪，

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