持续灌注和静态保存对中性粒细胞凋亡的影响

发布时间：2018-11-04 20:42

【摘要】：为研究体外循环机持续灌注、间断压力灌注、单纯低温保存犬离体肺对支气管肺泡灌洗液中中性粒细胞凋亡变化以及对肺泡巨噬细胞吞噬凋亡中性粒细胞的影响,探索最佳的离体肺保存方式,将30只Mongrel犬随机分成3组,为体外循环组、压力灌注组和低温保存组,每组10只。在保持机械通气的条件下,完整摘取双肺,3种不同方式保存离体肺,并按时间点留取支气管肺泡灌洗液标本。流式细胞仪检测犬离体肺支气管肺泡灌洗液中中性粒细胞凋亡,免疫组织化学法检测犬离体肺肺泡巨噬细胞对凋亡中性粒细胞吞噬。随着犬肺离体时间的推移,各实验组离体肺支气管肺泡灌洗液中凋亡的中性粒细胞明显减少,在每个时间点上,体外循环组中性粒细胞的凋亡最多,压力灌注组中次之,低温保存组中最少。各实验组吞噬凋亡中性粒细胞的巨噬细胞随着离体时间的延长呈逐渐减少的趋势,在肺离体各时间点上,体外循环组中巨噬细胞对凋亡中性粒细胞的吞噬清除优于间断压力灌注组和单纯低温保存组。采用体外循环机持续灌注犬离体肺,能增加中性粒细胞凋亡,促使吞噬细胞吞噬凋亡的中性粒细胞,避免发生凋亡延迟,从而减轻离体肺组织的缺血再灌注损伤,具有较好的肺保护作用。
[Abstract]:In order to study the effects of continuous perfusion of cardiopulmonary bypass (CPB), intermittent pressure perfusion and cryopreservation on the apoptosis of neutrophils in bronchoalveolar lavage fluid (BALF) and the phagocytosis of alveolar macrophages (AMs) in dogs. To explore the best method of lung preservation in vitro, 30 Mongrel dogs were randomly divided into three groups: cardiopulmonary bypass group, pressure perfusion group and hypothermia preservation group with 10 dogs in each group. Under the condition of maintaining mechanical ventilation, the lungs were removed completely, the isolated lungs were preserved in three different ways, and the bronchoalveolar lavage fluid specimens were collected according to the time points. The apoptosis of neutrophils in the bronchoalveolar lavage fluid was detected by flow cytometry, and the phagocytosis of apoptotic neutrophils by alveolar macrophages was detected by immunohistochemistry. The neutrophil apoptosis in bronchoalveolar lavage fluid (BALF) was significantly decreased in each experimental group with the passage of time in vitro. At each time point, neutrophil apoptosis was the most in cardiopulmonary bypass group, followed by pressure perfusion group. The lowest in cryopreservation group. The macrophages phagocytosis of apoptotic neutrophils in each experimental group showed a decreasing trend with the prolongation of the time in vitro. The phagocytosis of apoptotic neutrophils in cardiopulmonary bypass group was better than that in intermittent pressure perfusion group and cryopreservation group. Continuous perfusion of isolated canine lung by extracorporeal circulation can increase neutrophil apoptosis, promote phagocytosis of apoptotic neutrophil, avoid delayed apoptosis, and reduce ischemia-reperfusion injury in isolated lung tissue. It has better lung protective effect.
【作者单位】：昆明医科大学第一附属医院;
【基金】：国家自然科学基金资助项目(81060010)资助
【分类号】：R655.3

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