跑台运动对小鼠骨性关节炎的影响机制

发布时间：2018-11-08 21:15

【摘要】：研究运动对软骨细胞基质降解酶MMP13、Adamts4、Adamts5及软骨标记蛋白Col2、Col10、Runx2、Sox9的影响,为骨性关节炎运动医学角度提供科学依据。方法:对36只8周雄性小鼠,随机分成不手术对照组(C),手术自由活动组(F)和手术跑台组,手术为DMM关节炎手术,对照组不手术自由喂养,手术自由组进行DMM手术后自由喂养,手术跑台组进行DMM手术后5天后进行跑台,每天进行20m跑台训练,6周后处死取样。对3组小鼠膝关节进行石蜡包埋切片,通过fast green-Safranin O染色对关节炎进行评级,然后在通过Q-PCR和Western Blotting检测相关基因mRNA和蛋白水平的表达。结果:1)与C组比较,F组和R组关节均有不同级别损伤,而R关节受损程度更加严重。2)与C组和F组相比,R组MMP13mRNA表达均有非常显著性升高(P0.01),F组与C组无显著性差异;与C组和F组相比,R组Adamts4mRNA表达有均有非常显著性升高(P0.01),与C组相比,F组Adamts4mRNA也有显著性升高(P0.05);与C组和F组相比,R组Adamts5mRNA表达均有非常显著性升高(P0.01),F组与C组相比也有非常显著性升高(P0.01);与C组和F组相比,R组Col2mRNA表达有均有非常显著性降低(P0.01),F组与C组相比有非常显著性降低(P0.01)。3)与C组和F组相比,R组col2蛋白表达量均有非常显著性下降(P0.01),F组比C组的Col2蛋白表达量也有非常显著性下降(P0.01);与C组和F组相比,R组Sox9蛋白表达均有非常显著性下降(P0.01),F组比C组Sox9蛋白表达量也有非常显著性下降(P0.01);与C组和F组相比,R组Col10蛋白表达都有非常显著性增加(P0.01),F组比C组Col10蛋白表达量也有非常显著性增加(P0.01);与C组和F组相比,R组Runx2蛋白表达都有非常显著性增加(P0.01),F组比C组Runx2蛋白表达量也有非常显著性增加(P0.01)。结论:跑台运动加快骨性关节炎小鼠关节软骨的降解和钙化,加重了关节软骨损伤。
[Abstract]:To study the effects of exercise on chondrocyte matrix degrading enzyme (MMP13,Adamts4,Adamts5) and cartilage marker protein (Col2,Col10,Runx2,Sox9) in order to provide scientific basis for sports medicine of osteoarthritis. Methods: Thirty-six 8-week-old male mice were randomly divided into two groups: the non-operative control group (C), the free activity group (F) and the treadmill group (operation group). The operation was performed on DMM arthritis, and the control group was fed freely without operation. The patients in the free group were fed freely after DMM operation, and the patients in the operation platform group were given the treadmill 5 days after DMM operation, and 20 m treadmill training was carried out every day. After 6 weeks, the rats were killed and sampled. The knee joints of the three groups were stained with paraffin embedded sections. The arthritis was graded by fast green-Safranin O staining. The expression of mRNA and protein was detected by Q-PCR and Western Blotting. Results: 1) compared with group C, there were different grades of joint injury in group F and group R, and the degree of damage to joint of R was more serious. 2) compared with group C and group F, the expression of MMP13mRNA in group R was significantly higher than that in group C (P0.01). There was no significant difference between group F and group C; Compared with group C and group F, the expression of Adamts4mRNA in group R was significantly higher than that in group C (P0.01), and the expression of Adamts4mRNA in group F was significantly higher than that in group C (P0.05). Compared with group C and group F, the expression of Adamts5mRNA in group R was significantly higher than that in group C (P0.01), F and group C). Compared with group C and group F, the expression of Col2mRNA in group R was significantly lower than that in group C (P0.01), F) and group C (P0.01). 3) compared with group C and group F, the expression of Col2mRNA in group R was significantly lower than that in group C and group F. The expression of col2 protein in group R was significantly lower than that in group C (P0.01). Compared with group C and group F, the expression of Sox9 protein in group R was significantly decreased (P0.01), F group was significantly lower than that in group C (P0.01). Compared with group C and group F, the expression of Col10 protein in group R was significantly higher than that in group C (P0.01), F) and Col10 protein expression in group C (P0.01). Compared with group C and group F, the expression of Runx2 protein in group R was significantly higher than that in group C (P0.01), F) and Runx2 protein expression in group C (P0.01). Conclusion: treadmill exercise can accelerate the degradation and calcification of articular cartilage in osteoarthritis mice and aggravate the injury of articular cartilage.
【作者单位】：西北民族大学体育学院;华东师范大学青少年健康评价与运动干预教育部重点实验室;
【基金】：中央高校基本科研业务费专项资金项目(31920170089) 青少年健康评价与运动干预教育部重点实验室建设项目(40500-541235-14203/004)
【分类号】：R684.3;R87

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