三种犬离体肺保存方式对肺表面活性物质的影响

发布时间：2018-11-09 08:43

【摘要】：为研究3种不同的犬离体肺保存方式对支气管肺泡灌洗液中肺表面活性物质的影响。随机将30只Mongrel犬分成3组,为体外循环灌注组、间断压力灌注组和单纯低温保存组,每组10只。在保持机械通气的条件下,摘取犬双肺,3种不同方式保存离体肺,按时间点留取支气管肺泡灌洗液标本,BCA法测定总蛋白浓度,抗坏血酸法测定总磷脂的含量,薄层色谱分析测定二棕榈酸磷脂酰胆碱含量,酶联免疫吸附试验检测白介素(IL-1a/2/6/8)。随着犬肺离体时间的推移,各实验组离体肺支气管肺泡灌洗液中DPPC/PL的比值呈时间依赖性下降,各白介素/总蛋白比值呈时间依赖性升高。在同一时间点上,体外循环灌注组中DPPC/PL的比值最高,间断压力灌注组次之,单纯低温保存组最低;体外循环灌注组中各白介素/总蛋白的比值最低,间断压力灌注组次之,单纯低温保存组最高。采用体外循环机持续灌注犬离体肺,离体肺中可维持较高浓度的肺泡表面活性物质,维持肺泡表面张力,发挥肺保护作用,这可能与抑制炎症介质的释放有关。
[Abstract]:To study the effects of three different ways of preservation of canine lung on pulmonary surfactant in bronchoalveolar lavage fluid (BALF). Thirty Mongrel dogs were randomly divided into three groups: cardiopulmonary bypass group, intermittent pressure perfusion group and cryopreservation group. Under the condition of keeping mechanical ventilation, the canine lungs were harvested and preserved in three different ways. The bronchoalveolar lavage fluid samples were collected according to the time point, the total protein concentration was determined by BCA method, and the total phospholipid content was determined by ascorbic acid method. The content of phosphatidylcholine palmitate was determined by TLC and interleukin (IL-1a/2/6/8) was detected by enzyme linked immunosorbent assay (Elisa). The ratio of DPPC/PL in bronchoalveolar lavage fluid decreased in a time-dependent manner, and the ratio of interleukin / total protein increased in a time-dependent manner with the passage of isolated canine lung. At the same time point, the ratio of DPPC/PL was the highest in cardiopulmonary bypass group, followed by intermittent pressure perfusion group, and the lowest in cryopreservation group. The ratio of interleukin / total protein was the lowest in cardiopulmonary bypass group, followed by intermittent pressure perfusion group, and the highest in cryopreservation group. Extracorporeal circulation (CPB) was used to continuously infuse the isolated canine lung. The isolated lung could maintain a high concentration of alveolar surfactant, maintain the surface tension of alveoli, and play a protective role in the lung, which might be related to the inhibition of the release of inflammatory mediators.
【作者单位】：昆明医科大学第一附属医院;
【基金】：国家自然科学基金资助项目(81060010)资助
【分类号】：R655.3

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