可乐定通过激活胆碱能抗炎通路抑制肺损伤小鼠炎性反应

发布时间：2018-11-14 11:19

【摘要】：目的:探讨可乐定对肺损伤小鼠炎性反应的影响及其作用机制。方法:以脂多糖(lipopolysac charide,LPS)诱导的肺损伤小鼠为模型,静脉注射可乐定,取左肺上叶组织,检测肺湿干重比(W/D)和总肺含水量(TLW),ELISA试剂盒检测炎性因子IL-1β、IL-6和TNF-α的含量,RT-PCR和Western blot检测α7烟碱型乙酰胆碱受体(α7nAChR)、高迁移率族蛋白B1(HMGB1)表达的变化;鼻内吸入法将α7nAChR基因RNA干扰慢病毒载体导入肺损伤模型小鼠肺内,或者静脉注射HMGB1外源性蛋白,检测炎性因子和HMGB1的变化。结果:可乐定可减轻LPS诱导的小鼠肺损伤,并通过降低细胞因子IL-6、IL-10和TNF-α水平从而抑制LPS诱导的炎性反应;可乐定促进α7nAChR表达从而激活胆碱能抗炎通路,并抑制HMGB1的表达。结论:可乐定对肺损伤小鼠具有抗炎作用,这可能与激活胆碱能抗炎通路,抑制HMGB1的表达有关。
[Abstract]:Objective: to investigate the effect and mechanism of clonidine on inflammatory response in mice with lung injury. Methods: the model of lung injury induced by lipopolysaccharide (lipopolysac charide,LPS) was used to detect the inflammatory factor IL-1 尾 by intravenous injection of clonidine. The lung wet dry weight ratio (W / D) and total lung water content (TLW), ELISA) were measured. The content of IL-6 and TNF- 伪, the expression of 伪 7 nicotinic acetylcholine receptor (伪 7nAChR) and high mobility group protein B1 (HMGB1) were detected by RT-PCR and Western blot. The 伪 7nAChR gene RNA interfering lentivirus vector was introduced into the lung of lung injury model mice by nasal inhalation, or the exogenous protein of HMGB1 was injected intravenously to detect the changes of inflammatory factors and HMGB1. Results: clonidine could attenuate the lung injury induced by LPS and inhibit the inflammatory response induced by LPS by decreasing the levels of cytokines IL-6,IL-10 and TNF- 伪. Clonidine promoted 伪-7nAChR expression and activated cholinergic anti-inflammatory pathway and inhibited HMGB1 expression. Conclusion: clonidine has anti-inflammatory effect on lung injury mice, which may be related to activation of cholinergic anti-inflammatory pathway and inhibition of HMGB1 expression.
【作者单位】：南阳医专一附院麻醉科;南阳市中心医院麻醉科一部;
【分类号】：R614

【参考文献】