两种方法建立兔腰椎间盘退变模型

发布时间：2018-11-14 17:50

【摘要】：背景:国内外学者对椎间盘退变动物模型的建立做了大量工作,通过改变椎间盘力学特性、纤维环损伤、酶化学法等多种建模方式模拟椎间盘退变的病理表现。目的:通过髓核抽吸法和纤维环薄层切除法建立椎间盘退变模型,比较两种方法的造模效果。方法:取6月龄新西兰大白兔20只,通过手术暴露L3-4、L4-5及L5-6椎间盘,随机使用髓核抽吸法和纤维环薄层切除法对L3-4、L4-5椎间盘进行干预:(1)髓核抽吸组——利用16 G针头在椎间盘纤维环穿刺并负压抽吸10 s;(2)纤维环薄层切除组——在显微镜下辅助下,在兔椎间盘纤维环上切除1 mm×2 mm范围的约1/2层纤维环组织(避免切透全层)。于造模第1,3,5,9,13周进行核磁共振、X射线检查及椎间盘Masson染色病理学观察。结果与结论:(1)X射线检查:纤维环薄层切除组椎间隙高度自术后第1周起便发生不同程度降低;髓核抽吸组椎间隙在术后1周及3周轻微降低,自第5周起发生较明显降低;纤维环薄层切除组术后第3,5,9周椎间隙高度指数百分比高于髓核抽吸组(P0.05);(2)核磁共振检查:两种方法均诱导相应椎间盘发生退变,各组随时间推移椎间盘退变程度逐渐加重,并于9周后均达到4级退变;髓核抽吸组术后1,3,5周的椎间盘退变程度重于纤维环薄层切除组(P0.05);(3)病理学观察:随时间推移,两组椎间盘髓核及纤维环退变程度逐渐加重;(4)结果表明:髓核抽吸法和纤维环薄层切除法均能诱导椎间盘退变,纤维环薄层切除法在5周内造成的椎间盘退变程度较髓核抽吸法轻,纤维环薄层切除法在纤维环上所造成的标示部位可作为纤维环修补研究的靶点。
[Abstract]:Background: scholars at home and abroad have done a lot of work on the establishment of animal model of intervertebral disc degeneration. By changing the mechanical properties of intervertebral disc, the damage of fibrous ring, enzyme chemical method and other modeling methods to simulate the pathological manifestations of disc degeneration. Objective: to establish the model of disc degeneration by the method of nucleus pulposus aspiration and thin-layer excision of fibrous ring, and compare the effect of the two methods. Methods: twenty New Zealand white rabbits aged 6 months were exposed to L3-4 and L4-5 and L5-6 intervertebral discs by surgery. L3-4 was randomly treated by nucleus pulposus aspiration and thin-layer annular excision. L4-5 intervertebral disc intervention: (1) Nucleus pulposus aspiration group-16G needle in disc fiber ring puncture and suction negative pressure for 10 seconds; (2) Thin-layer excision of the fibrous annulus: with the aid of microscope, about 1 / 2 layer of fibrous annular tissue in the range of 1 mm 脳 2 mm was removed on the rabbit intervertebral disc annulus (avoiding cutting through the whole layer). Nuclear magnetic resonance (MRI), X-ray examination and Masson staining of intervertebral disc were performed at 913 weeks. Results and conclusion: (1) X-ray examination: the height of intervertebral space in thin layer excision group decreased in varying degrees from the first week after operation. The intervertebral space decreased slightly at 1 and 3 weeks postoperatively in the nucleus pulposus aspiration group and decreased significantly at the 5th week, and the percentage of the intervertebral space height index in the thin-layer excision group was higher than that in the nucleus pulposus suction group at the 9th week after operation (P0.05). (2) MRI: the degeneration of the intervertebral disc was induced by both methods, and the degree of degeneration of the intervertebral disc increased gradually with the passage of time, and reached grade 4 after 9 weeks. The degenerative degree of intervertebral disc in the pulposus aspiration group was more serious than that in the thin-layer excision group (P0.05); (3). The degeneration degree of the nucleus pulposus and the fibrous ring of the intervertebral disc increased gradually with the passage of time. (4) the results showed that the disc degeneration could be induced by the pulposus aspiration method and the thin-layer excision method, and the degree of disc degeneration caused by the thin-layer excision of the fibrous ring in 5 weeks was less than that in the nucleus pulposus aspiration method. The marking site caused by thin-layer excision of fiber ring on the fiber ring can be used as a target for the repair of fiber ring.
【作者单位】：新疆医科大学第六附属医院脊柱外科;
【分类号】：R681.53;R-332

【相似文献】