膜联蛋白A2、上皮细胞钙黏蛋白及波形蛋白在肝内胆管结石相关肝内胆管癌中的表达及意义

发布时间：2018-11-14 20:10

【摘要】：目的:探讨膜联蛋白A2(annexin A2)与上皮-间质转化(EMT)标志物上皮细胞钙黏蛋白(E-cadherin)及波形蛋白(vimentin)在肝内胆管结石相关肝内胆管癌中的表达及意义。方法:用免疫组化检测46例肝内胆管结石相关肝内胆管癌组织(肿瘤组)、50例单纯肝内胆管结石慢性炎症胆管组织(炎症组)及35例来自血管瘤旁和肝外伤的正常胆管组织(正常组)中annexin A2、E-cadherin、vimentin的表达,比较各组3种蛋白表达的差异,并分析3种蛋白的表达与肿瘤组患者临床病理特征及预后的关系。结果:annexin A2与vimentin的阳性表达率在肿瘤组、炎症组、正常组均呈依次明显降低(69.6%vs.36.0%vs.11.4%;54.3%vs.28.0%vs.8.6%,均P0.05),而E-cadherin的阳性表达率则相反(21.7%vs.48.0%vs.71.4%,均P0.05);在胆管癌组织中,annexin A2与vimentin的表达呈正相关(r=0.627,P0.05),E-cadherin的表达与annexin A2、vimentin的表达均呈负相关(r=-0.682;r=-0.575,均P0.05)。annexin A2、vimentin的表达与肿瘤的分化程度、淋巴结转移、TNM分期有关,E-cadherin的表达与肿瘤的分化程度、淋巴结转移有关(均P0.05)。肝内胆管结石相关肝内胆管癌患者中,annexin A2和vimentin各自阳性者的生存率低于其各自阴性者,E-cadherin阴性者生存率低于其阳性者(均P0.05)。结论:annexin A2/EMT通路在肝内胆管结石相关肝内胆管癌的发生和发展中可能至关重要,annexin A2、E-cadherin及vimentin的表达与肿瘤的恶性程度和患者预后密切相关。
[Abstract]:Objective: to investigate the expression and significance of annexin A2 and epithelial cell cadherin (E-cadherin) and vimentin (vimentin) in intrahepatic cholelithiasis associated with intrahepatic cholangiocarcinoma. Methods: 46 cases of intrahepatic bile duct calculi associated with intrahepatic cholangiocarcinoma (tumor group) were detected by immunohistochemistry. The expression of annexin A _ 2 E-cadherin vimentin was detected in 50 cases of chronic inflammatory bile duct tissue (inflammation group) and 35 cases of normal bile duct tissue (normal group) from hemangioma and hepatic trauma. The relationship between the expression of three proteins and the clinicopathological features and prognosis of tumor patients was analyzed. Results: the positive expression rates of annexin A2 and vimentin in tumor group, inflammatory group and normal group were significantly decreased in turn (69.6 vs.36.0vs.11.4). The positive expression rate of E-cadherin was opposite (21.7vs.48.0 vs 71.4, P0.05). There was a positive correlation between the expression of annexin A2 and vimentin (r 0.627 P 0.05), and a negative correlation between the expression of E-cadherin and the expression of annexin A 2 vimentin (r = 0.682). The expression of). Annexin A2vimentin was related to the degree of differentiation, lymph node metastasis and TNM stage, and the expression of E-cadherin was related to the degree of differentiation and metastasis of lymph node (P0.05). In patients with intrahepatic cholelithiasis associated intrahepatic cholangiocarcinoma, the survival rate of annexin A2 and vimentin positive patients was lower than that of their respective negative patients, and the survival rate of E-cadherin negative patients was lower than that of E-cadherin negative patients (P0.05). Conclusion: the annexin A2/EMT pathway may play an important role in the occurrence and development of intrahepatic cholangiocarcinoma associated with intrahepatic cholelithiasis. The expression of annexin A2E-cadherin and vimentin is closely related to the malignancy of the tumor and the prognosis of the patients.
【作者单位】：湖南师范大学第一附属医院/湖南省人民医院肝胆外科;
【基金】：湖南省教育厅高校科研计划资助项目(15C0836) 湖南省长沙市科学技术局科技计划资助项目(K1203042-31) 2016年湖南省研究生科研创新资助项目(CX2016B224)
【分类号】：R657.42;R735.8

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