迷走神经电刺激对脑外伤昏迷大鼠意识及前额叶皮质γ-氨基丁酸b1受体表达的影响

发布时间：2018-11-24 19:01

【摘要】：目的研究迷走神经电刺激(VNS)对脑外伤(TBI)昏迷大鼠的促醒效果及其机制。方法 168只健康Sprague-Dawley大鼠随机分为空白组、TBI组、拮抗剂组和VNS组,每组42只。空白组不做任何处理,TBI组、VNS组、拮抗剂组撞击法复制脑外伤模型,造模后昏迷至少30 min的大鼠入选。VNS组予VNS,拮抗剂组侧脑室注射Orexin A受体1拮抗剂SB334867后加VNS,TBI组予假VNS。分别于干预后6 h、12 h、24 h观察大鼠意识水平,应用免疫组化、Western blotting检测前额叶皮质γ-氨基丁酸b1受体(GABAb1R)表达水平。结果最终空白组42只、TBI组11只、拮抗剂组13只、VNS组28只大鼠觉醒。Western blotting显示,干预后12 h、24 h,GABAb1R蛋白表达TBI组拮抗剂组空白组VNS组(F60.412,P0.001)。免疫组化显示,GABAb1R蛋白表达TBI组拮抗剂组VNS组空白组(H=15.121,P=0.002),但不同时间点无显著性差异(H=3.028,P=0.220)。结论 VNS可促TBI昏迷大鼠觉醒,其促醒机制可能与Orexin A介导大鼠前额叶皮质GABAb1R表达水平下调有关。
[Abstract]:Objective to study the effect and mechanism of vagal nerve stimulation (VNS) on brain injury in (TBI) coma rats. Methods 168 healthy Sprague-Dawley rats were randomly divided into control group, TBI group, antagonist group and VNS group with 42 rats in each group. TBI group, VNS group, antagonist group were used to make brain injury model, and the rats in the VNS group were selected to be in coma for at least 30 min. The VNS group was given VNS, antagonist group after injection of Orexin A receptor 1 antagonist SB334867 into the lateral ventricle and VNS, was added to the brain. TBI group was given false VNS. The level of consciousness was observed at 12 h and 24 h after intervention, and the expression of 纬 -aminobutyric acid b1 receptor (GABAb1R) in prefrontal cortex was detected by immunohistochemical, Western blotting. Results 42 rats in the blank group, 11 rats in the TBI group, 13 rats in the antagonist group and 28 rats in the VNS group had awakened. Western blotting. The expression of GABAb1R protein in the control group (F60.412P0.001) was detected 24 hours after the intervention. The expression of GABAb1R protein in the TBI group was significantly higher than that in the control group (F60.412P0.001). Immunohistochemical staining showed that GABAb1R protein was expressed in TBI antagonist group, VNS group was blank group (Hn15.121 P0. 002), but there was no significant difference at different time points (H0. 028, P0. 220). Conclusion VNS can promote the arousal of TBI coma rats, and its mechanism may be related to the down-regulation of GABAb1R expression in prefrontal cortex induced by Orexin A.
【作者单位】：南昌大学第一附属医院康复医学科;
【基金】：国家自然科学基金项目(No.81260295)
【分类号】：R651.15

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