β-磷酸三钙预构中心血管化人工骨的实验研究

发布时间：2018-11-25 21:22

【摘要】：目的:观察β-磷酸三钙构建中心血管化人工骨的效果,探索人工骨血管化机制及血管植入的可行性。方法:将兔的腰背动脉解剖分离形成血管束,实验组将血管束植入人工骨侧槽内,并用自体微小颗粒骨填满,埋入背阔肌肌袋内,对侧不植入血管作为对照。术后4、8、12周行螺旋CT、X线和组织学检查。结果:实验组人工骨形成类似滋养孔的结构,有大量新生血管自中央向四周生长,4周时开始出现新生骨,12周新生骨更趋于成熟。对照组有血管自周围长入,新生骨少且不成熟。术后4周,实验组和对照组CT值、X线及组织学评分比较,差异无统计学意义;术后8、12周,实验组和对照组CT值、X线及组织学评分比较差异有统计学意义(P0.05)。结论:血管植入人工骨可显著促进人工骨血管化,该方法可作为构建血管化骨模型。
[Abstract]:Aim: to observe the effect of 尾-tricalcium phosphate (尾-tricalcium phosphate) on cardiovascular artificial bone, and to explore the mechanism of vascularization and the feasibility of vascular implantation. Methods: the rabbit lumbar dorsal artery was dissected to form a vascular bundle. In the experimental group, the vascular bundle was implanted into the lateral groove of the artificial bone, filled with autogenous microgranular bone, embedded in the latissimus dorsi muscle bag, and not implanted in the contralateral side as the control. Spiral CT,X line and histological examination were performed at 12 weeks after operation. Results: in the experimental group, the artificial bone formed a structure similar to the trophoblast, and a large number of neovascularization vessels grew from the center to the four sides. At the 4th week, the new bone began to appear, and at the 12th week, the new bone tended to mature. In the control group, the blood vessels grew from around, the new bone was little and immature. There was no significant difference in CT, X-ray and histological score between the experimental group and the control group 4 weeks after operation, but there was significant difference in the CT value, X-ray and histological score between the experimental group and the control group at 812 weeks after operation (P0.05). Conclusion: vascular implantation of artificial bone can significantly promote the vascularization of artificial bone, and this method can be used as a vascularized bone model.
【作者单位】：连云港市第二人民医院口腔科;南京市口腔医院病理科;
【分类号】：R318.17;R687

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