大鼠肺损伤模型建立及一氧化碳肺保护的作用机制

发布时间：2018-12-09 21:18

【摘要】：目的探讨建立大鼠肺损伤模型一氧化碳(CO)肺保护的作用机制。方法将28只SD大鼠按抽签的方式分为对照组和研究组,均14只,其中7只为供体,7只为受体。摘除供体大鼠的肺部,将对照组的供肺进行12 h的保存,再进行1 h的静脉血灌注。研究组在移植的过程中全程吸入低浓度的CO,至再灌注后1 h结束,余同对照组。抽取流入及流出供肺的血液进行对血气分析,包括静脉血CO_2分压(Pv CO_2)、静脉血氧分压(Pv O_2)、酸碱度(p H)、血红蛋白(Hb)、动脉血氧分压(Pa CO_2)、动脉血氧分压(Pa O_2)的测定,20 min/次。再灌注期间对气道峰压(Paw P)、肺动脉压(m PAP)、Pa O_2进行测定;再灌注完成后对肺组织干湿比(W/D)、白细胞介素(IL)-8、丙二醛(MDA)、髓过氧化物酶(MPO)进行测定,20 min/次。结果各时段的Pv CO_2、Pv O_2、p H、Hb水平均无明显变化(P0.05);随着灌注时间的增加,Pa CO_2、m PAP、Paw P水平逐渐上升,而Pa CO_2水平逐渐下降;在第40、60 min时,研究组的Paw P水平明显低于对照组(P0.05);在第20、40、60 min时,研究组Pa O_2水平均明显高于对照组、m PAP低于对照组(P0.05);研究组的IL-8、MDA、W/D、MPO水平均明显低于对照组(P0.05)。结论在肺移植的过程中全程吸入低剂量的CO,可有效改善供肺功能,减轻供肺的缺血再灌注损伤,对肺保护具有重要意义。
[Abstract]:Objective to investigate the protective mechanism of carbon monoxide (CO) (CO) in rat lung injury model. Methods 28 SD rats were divided into control group (n = 14) and study group (n = 14). The donor lungs of the control group were preserved for 12 h and the venous blood was perfused for 1 h. The study group inhaled low concentration of CO, in the whole course of transplantation and ended 1 hour after reperfusion, the remaining group was the same as the control group. Blood gas was analyzed by extracting blood from the blood flowing into and out of the lungs, including CO_2 partial pressure (Pv CO_2), partial pressure of oxygen (Pv O\ -2), pH (p H), hemoglobin (Hb), Arterial partial pressure of oxygen (Pa CO_2) and arterial partial pressure of oxygen (Pa O\ -2) were measured for 20 min/. The peak airway pressure (Paw P),) pulmonary artery pressure (m PAP), Pa O 2) was measured during reperfusion. Lung dry-wet ratio (W / D), interleukin-8 (IL-8), malondialdehyde (MDA) (MDA), myeloperoxidase (MPO) were measured for 20 min/ after reperfusion. Results there was no significant change in the level of Pv CO_2,Pv O2HP HB in all periods (P0.05), but with the increase of perfusion time, the level of Pa CO_2,m PAP,Paw P gradually increased, while the level of Pa CO_2 decreased gradually. The level of Paw P in the study group was significantly lower than that in the control group at the 40th min (P0.05), the Pa O\ -2 level in the study group was significantly higher than that in the control group at 4060 min (P0.05), and that in the control group was significantly lower than that in the control group (P0.05). The level of IL-8,MDA,W/D,MPO in the study group was significantly lower than that in the control group (P0.05). Conclusion inhalation of low dose CO, during the whole course of lung transplantation can effectively improve donor lung function and alleviate ischemia reperfusion injury of donor lung, which is of great significance for lung protection.
【作者单位】：南昌大学第四附属医院麻醉科;南昌大学第四附属医院手术室;
【基金】：江西省卫生厅课题(No.20123082)
【分类号】：R-332;R655.3

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