骨细胞外基质有机相网络微纳结构的研究

发布时间：2018-12-14 09:03

【摘要】：细胞微环境能够通过各种理化因素对细胞的日常活动,如蛋白质表达、有丝分裂、分化、凋亡等进行精细地调控,对维持细胞和组织的正常形态和功能不可或缺。在物理因素中,针对形貌因素的研究正方兴未艾。在骨细胞外基质中存在着由胶原纤维、蛋白聚糖复合物等有机物构成的多级次网络结构,对骨组织理化特性的维持,多种骨细胞各种功能的调控起到不可或缺的作用。本文聚焦于骨细胞外基质有机相微观水平的形貌,力求从这一角度增加对骨组织微环境的认识。首先我们通过原子力显微镜和扫描电子显微镜表征了网络的结构；其次,基于图像分析算法建立了分析和提取随机网络几何特征参数的方法；然后构建了计算机几何网络模型,并利用静电纺丝构建了微米级体外网络模型；最后利用水凝胶构建了纳米级体外网络模型,并在水凝胶模型上研究了纳米级网络交联度对间充质干细胞铺展的作用,为进一步理解骨组织有机相多级次网络在病理生理过程所起的作用和合理设计生物工程支架提供了基础。
[Abstract]:The microenvironment can regulate the daily activities of cells through various physical and chemical factors such as protein expression mitosis differentiation apoptosis and so on. It is indispensable to maintain the normal morphology and function of cells and tissues. Among the physical factors, the research on morphologic factors is in the ascendant. In the extracellular matrix of bone, there is a multilevel secondary network composed of collagen fiber, proteoglycan complex and other organic compounds, which plays an indispensable role in maintaining the physical and chemical properties of bone tissue and regulating various functions of various bone cells. This paper focuses on the morphology of the organic phase of the extracellular matrix of bone in order to enhance the understanding of the microenvironment of bone tissue from this point of view. Firstly, the structure of the network is characterized by atomic force microscope and scanning electron microscope. Secondly, the method of analyzing and extracting geometric characteristic parameters of random network is established based on image analysis algorithm. Then the computer geometric network model is constructed, and the micron scale external network model is constructed by electrostatic spinning. Finally, a nanoscale in vitro network model was constructed using hydrogel, and the effect of the degree of network crosslinking on the spread of mesenchymal stem cells was studied on the hydrogel model. It provides a basis for further understanding the role of bone organic multistage sub-network in the pathophysiological process and the rational design of bioengineering scaffolds.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R68

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