右美托咪定对糖尿病大鼠体液免疫及补体的影响

发布时间：2018-12-16 23:12

【摘要】：目的:通过腹腔注射链脲佐菌素建立糖尿病大鼠模型,观察右美托咪定对糖尿病大鼠体液免疫功能及补体功能的影响,探讨右美托咪定在糖尿病大鼠体液免疫功能调节中的作用。方法:选择健康成年雄性SD大鼠72只,10~12周龄,体重300~350g,采用随机数字表法,将其随机均分为3组:对照组(C组)、糖尿病组(DM组)和右美托咪定+糖尿病组(DEX组),每组24只。DM组和DEX组采用链脲佐菌素(STZ)一次性腹腔注射60mg/kg建立糖尿病大鼠模型,于糖尿病模型稳定后4W时三组大鼠均实施剖腹探查术,维持手术探查时间为30min。DEX组于手术开始前经10min静脉输注右美托咪定负荷剂量1μg/kg,随后以5μg/kg/h(容量1ml/h)的速率维持4h,C组和DM组均给予等容量生理盐水。分别于术前(T0)、术毕、术后24h和72h时(T1~T3)每组随机取6只大鼠采集血样,测定血糖,并采用Elisa法检测血清IgG、IgA及IgM和补体CH50、C3及C4的浓度;分别于T0~T3时采血结束后处死大鼠,处死前称重,解剖分离脾脏组织称重,计算脾脏指数[脾脏重量(mg)/大鼠重量(g)]。结果:(1)与C组比较,DM组和DEX组T0~T3时血糖浓度升高(P0.05);与DM组比较,DEX组T1~T3时血糖浓度下降(P0.05),T0时差异无统计学意义(P0.05)。(2)与C组比较,DM组和DEX组T0~T3时血清IgG、IgM浓度降低,IgA浓度升高(P0.05);与DM组比较,DEX组T1~T3时血清IgG、IgM浓度升高,IgA浓度降低(P0.05)。(3)与C组比较,DM组和DEX组T0~T3时血清CH50、C3及C4浓度均升高(P0.05);与DM组比较,DEX组T1~T3时血清CH50、C3及C4浓度均降低(P0.05)。(4)与C组比较,DM组和DEX组T0~T3时脾脏指数降低(P0.05);与DM组比较,DEX组T1~T3时脾脏指数升高(P0.05)。结论:糖尿病可诱发大鼠体液免疫功能下降及补体功能异常,手术创伤后体液免疫及补体功能进一步下降,而右美托咪定可改善糖尿病大鼠围术期体液免疫及补体功能。
[Abstract]:Objective: to observe the effect of dexmetomidine on humoral immune function and complement function of diabetic rats by intraperitoneal injection of streptozotocin. To investigate the role of dexmetidine in the regulation of humoral immune function in diabetic rats. Methods: 72 healthy adult male SD rats were randomly divided into three groups: control group (group C), diabetes mellitus group (DM group) and dexmetomidine diabetes group (DEX group). 24 rats in each group were treated with streptozotocin (STZ) intraperitoneal injection of streptozotocin (STZ) to establish diabetic model in DM group and DEX group. The exploration time of operation was maintained at 1 渭 g / kg by intravenous infusion of dexmetomidine through 10min before the operation in 30min.DEX group, and then at the rate of 5 渭 g/kg/h (volume 1ml/h) for 4 h. Group C and group DM were given the same volume of normal saline at the rate of 5 渭 g/kg/h (volume 1ml/h). Blood samples were collected from 6 rats in each group at pre-operation (T0), at the end of operation, 24h and 72h after operation (T1~T3), and the serum IgG,IgA and IgM, complement CH50,C3 and C4 were measured by Elisa method. The rats were killed at the end of T0~T3 and weighed before death. Spleen index [spleen weight (mg) / rat weight (g)] was measured by anatomical separation of spleen tissue. Results: (1) compared with group C, the concentration of blood glucose in DM group and DEX group was higher than that in group C (P0.05). Compared with DM group, the blood glucose concentration in DEX group decreased at T1~T3 (P0.05), but there was no significant difference at T0 (P0.05). (2). Compared with C group, the serum IgG,IgM concentration decreased and IgA concentration increased in DM group and DEX group (P0.05). Compared with the DM group, the serum IgG,IgM concentration increased and the IgA concentration decreased in the DEX group (P0.05). (3). Compared with the C group, the CH50,C3 and C4 levels in the DM group and DEX group increased significantly (P0.05). Compared with DM group, the serum CH50,C3 and C4 levels in DEX group were lower than those in C group (P0.05). (4). Compared with C group, the spleen index of DM group and DEX group was lower than that of C group (P0.05). Compared with DM group, the spleen index in DEX group was higher than that in T1~T3 group (P0.05). Conclusion: diabetes can induce decreased humoral immune function and abnormal complement function in rats. The humoral immunity and complement function of diabetic rats are further decreased after operation, while dexmetomidine can improve the humoral immunity and complement function of diabetic rats during perioperative period.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614

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