坐骨神经成纤维细胞修复周围神经缺损
发布时间：2019-02-16 02:30
【摘要】：坐骨神经成纤维细胞是正常神经组织中含量丰富的细胞,有着重要的功能作用。有研究表明乳腺等组织中间质成纤维细胞和其上皮共移植可以更好的促进组织器官的重塑。周围神经组织中的成纤维细胞对于周围神经再生起着何种作用,目前尚不明确。目的:我们想探究在周围神经离断伤后,其组织中的成纤维细胞在神经再生过程中的作用及其可能的机制。方法:本研究拟通过单纯的坐骨神经成纤维细胞移植或者坐骨神经成纤维细胞和许旺细胞共移植能否更好的促进神经的再生。首先,用PUR导管构建一个5mm神经缺损模型,将六组细胞分别注入导管并回植桥接坐骨神经缺损;六组细胞分别是:纯matrigel组(control blank),坐骨神经成纤维细胞组(SN-Fb),皮肤成纤维细胞组(Dm-Fb)坐骨神经成纤维细胞和许旺细胞1:1混合组(SN-FbSC1:1),坐骨神经成纤维细胞和许旺细胞1:2混合组(SN-FbSC1:2),坐骨神经许旺细胞组(SN-SC)。三个月后,对再生的神经进行组织学检测(轴突、许旺细胞及细胞外基质的免疫荧光染色)和靶器官功能学的检测(肌肉湿重统计及Masson染色)。结果:单纯的坐骨神经成纤维细胞回植产生过多的细胞外基质占位,阻碍神经再生。Fb与SC以1:2比例回植到缺损的神经中,从再生的神经轴突统计和腓肠肌萎缩及纤维化程度上,均和单纯的SC回植无差异,在再生许旺细胞数量的统计中优于单纯的SC回植。在体外细胞分泌的神经营养因子检测中,SN-FbSC1:2组均高于SN-SC组。结论:Fb与SC以1:2比例回植到缺损的神经中,修复效果优于单纯的许旺细胞回植,因此Fb在神经损伤修复过程中可能与SC起到协同作用,共同促进神经轴突的再生。
[Abstract]:Sciatic nerve fibroblasts are abundant cells in normal nerve tissue and have important function. Studies have shown that cotransplantation of stromal fibroblasts and their epithelium can promote the remodeling of tissues and organs. The role of fibroblasts in peripheral nerve regeneration is unclear. Aim: to explore the role of fibroblasts in the process of nerve regeneration and its possible mechanism after peripheral nerve injury. Methods: in this study, whether the sciatic nerve fibroblast transplantation or the sciatic nerve fibroblast and Schwann cell co-transplantation can better promote nerve regeneration. Firstly, a model of 5mm nerve defect was constructed with PUR catheter. The six groups of cells were injected into the catheter and transplanted back to graft the sciatic nerve defect. The six groups of cells were: (control blank), sciatic nerve fibroblast group (SN-Fb), skin fibroblast group (Dm-Fb), sciatic nerve fibroblast and Schwann cell 1:1 mixture group (SN-FbSC1:1). Sciatic nerve fibroblasts and Schwann cells were mixed at 1:2 (SN-FbSC1:2) and sciatic nerve Schwann cells (SN-SC). Three months later, the regenerated nerves were examined for histology (immunofluorescence staining of axons, Schwann cells and extracellular matrix) and for the function of target organs (muscle wet weight statistics and Masson staining). Results: the reimplantation of sciatic nerve fibroblasts resulted in too much extracellular matrix occupation, which blocked the regeneration of nerve. Fb and SC were implanted back into the defective nerve at 1:2. From the statistics of regenerated nerve axons and the degree of gastrocnemius atrophy and fibrosis, there was no difference between the regenerated nerve axons and the degree of atrophy and fibrosis of gastrocnemius muscle, which was superior to that of SC replantation in the statistics of the number of regenerated Schwann cells. The level of neurotrophic factor in SN-FbSC1:2 group was higher than that in SN-SC group. Conclusion: the repair effect of Fb and SC is better than that of Schwann cell replantation in proportion of 1:2 to the defective nerve. So Fb may play a synergistic role with SC in the repair of nerve injury and promote the regeneration of nerve axons.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R688
[Abstract]:Sciatic nerve fibroblasts are abundant cells in normal nerve tissue and have important function. Studies have shown that cotransplantation of stromal fibroblasts and their epithelium can promote the remodeling of tissues and organs. The role of fibroblasts in peripheral nerve regeneration is unclear. Aim: to explore the role of fibroblasts in the process of nerve regeneration and its possible mechanism after peripheral nerve injury. Methods: in this study, whether the sciatic nerve fibroblast transplantation or the sciatic nerve fibroblast and Schwann cell co-transplantation can better promote nerve regeneration. Firstly, a model of 5mm nerve defect was constructed with PUR catheter. The six groups of cells were injected into the catheter and transplanted back to graft the sciatic nerve defect. The six groups of cells were: (control blank), sciatic nerve fibroblast group (SN-Fb), skin fibroblast group (Dm-Fb), sciatic nerve fibroblast and Schwann cell 1:1 mixture group (SN-FbSC1:1). Sciatic nerve fibroblasts and Schwann cells were mixed at 1:2 (SN-FbSC1:2) and sciatic nerve Schwann cells (SN-SC). Three months later, the regenerated nerves were examined for histology (immunofluorescence staining of axons, Schwann cells and extracellular matrix) and for the function of target organs (muscle wet weight statistics and Masson staining). Results: the reimplantation of sciatic nerve fibroblasts resulted in too much extracellular matrix occupation, which blocked the regeneration of nerve. Fb and SC were implanted back into the defective nerve at 1:2. From the statistics of regenerated nerve axons and the degree of gastrocnemius atrophy and fibrosis, there was no difference between the regenerated nerve axons and the degree of atrophy and fibrosis of gastrocnemius muscle, which was superior to that of SC replantation in the statistics of the number of regenerated Schwann cells. The level of neurotrophic factor in SN-FbSC1:2 group was higher than that in SN-SC group. Conclusion: the repair effect of Fb and SC is better than that of Schwann cell replantation in proportion of 1:2 to the defective nerve. So Fb may play a synergistic role with SC in the repair of nerve injury and promote the regeneration of nerve axons.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R688
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