主动脉夹层Stanford A型microRNA的表达调控机制分析

发布时间：2019-02-27 16:25

【摘要】：目的:通过病例对照研究主动脉血管壁中膜组织microRNA(miRNA)的表达差异,探索与主动脉夹层发生发展相关的mi RNAs及其可能的作用靶点及信号通路。方法:选取2013年9月至2014年2月期间在深圳市孙逸仙心血管医院行外科手术治疗的Stanford A型主动脉夹层患者的主动脉血管壁中膜,其中病变主动脉血管壁中膜5例(A组),病变主动脉血管壁远端相对正常血管壁中膜5例(B组)。对照组C组为行冠状动脉旁路移植术治疗的患者主动脉打孔处血管壁中膜5例,对照组选取需排除主动脉相关疾病。所选样本均取自我国南方汉族人群男性患者的血管壁中膜组织。基于Agilent人miRNA芯片技术检测分析3组样本差异表达的miRNA,并对其可能的靶基因及信号通路进行相关分析。结果:病例组(A组、B组)与对照组(C组)患者的年龄、体重指数、吸烟史、嗜酒史比较,差异均无统计学意义(P0.05)。经Agilent芯片检测分析,结果显示:A组、B组与C组比较共有89个差异表达1.5倍的miRNAs,A组与B组之间共有13个差异表达1.5倍的mi RNAs。对差异表达的mi RNAs的靶基因预测及信号通路分析显示,有16个差异表达的miRNAs分别涉及PTEN、BCL2、RAS、MAPK1等23个靶基因,与MAPK信号通路、粘着斑、肌动蛋白骨架调节等11条信号通路相关。结论:1、粘着斑和MAPK信号通路在主动脉夹层的发生发展可能有重要的调节作用。2、细胞凋亡可能与主动脉夹层的发生、发展密切相关。3、对主动脉中膜组织mi RNAs的差异表达及调控机制的研究对于探索主动脉夹层发生发展机制具有重要意义。
[Abstract]:Aim: to study the difference of microRNA (miRNA) expression in aortic mesangial tissue by case-control study, and to explore the role of mi RNAs in the pathogenesis and development of aortic dissection and its possible target and signal pathway. Methods: from September 2013 to February 2014, the media of aortic wall of Stanford A aortic dissection patients who underwent surgical treatment in Shenzhen Sun Yat Xian Cardiovascular Hospital, including 5 cases of diseased aortic media (group A), were selected. The distal wall of the diseased aorta was relative to the media of the normal vascular wall in 5 cases (group B). Control group C (5 cases) were treated with coronary artery bypass grafting (CABG), and aortic associated diseases were excluded in the control group (5 cases). All the selected samples were taken from the vascular wall media of male patients with Han nationality in southern China. The differentially expressed miRNA, of three groups of samples was detected and analyzed based on Agilent human miRNA chip technique and its possible target gene and signal pathway were analyzed. Results: there was no significant difference in age, body mass index (BMI), smoking history and drinking history between patients (group A, B) and control group (group C) (P0.05). The results of Agilent chip analysis showed that there were 89 different expression of mi RNAs. between group A, group B and group C, and there were 13 differentially expressed mi RNAs. 1.5 times between group A and group B. The target gene prediction and signal pathway analysis of differentially expressed miRNAs showed that there were 16 differentially expressed miRNAs involving 23 target genes, including PTEN,BCL2,RAS,MAPK1, MAPK signaling pathway, adhesion spot, and so on. The regulation of actin cytoskeleton is related to 11 signal pathways. Conclusion: 1. Plaque of adhesion and MAPK signaling pathway may play an important role in the occurrence and development of aortic dissection. 2. Apoptosis may be closely related to the occurrence and development of aortic dissection. The study on the differential expression and regulation of mi RNAs in aortic media is of great significance for exploring the pathogenesis of aortic dissection.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R654.3

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