缺氧诱导因子-1α在脑缺血中的作用及其机制研究

发布时间：2019-03-08 15:19

【摘要】：目的研究在脑缺血过程中HIF-1α、VEGF因子的表达及其作用机制。方法选取60只成年健康雄性SD大鼠,体重250~300g,清洁级。将大鼠随机分为两组:实验组和对照组,每组各30只。造模取得成功2h后,实验组注射HIF-1α质粒液,对照组注射同等量的PBS缓冲液,按照注射后时间不同分为6h,24h,48h,72h,7d五个时间点,每个时间点亚组为6只大鼠。采用TTC染色法观察各组大鼠脑梗塞面积,来判断脑组织缺血程度;采用免疫印迹试验检测脑组织梗塞区中HIF-1α和VEGF的蛋白表达水平。结果1 TTC染色法观察各组大鼠脑梗塞面积与对照组相比,实验组6h、24h、48h时间点上梗塞面积无明显差异(P0.05),而在72h、7d时间点上有差异,其中7d时差异更为显著(P0.01)。2注射HIF-1α质粒液对大鼠死亡率的影响大鼠在缺血再灌注后注射HIF-1α质粒液,在4h~60h之间死亡率最高,若存活时间超过72h死亡率低(P0.01)。3免疫印迹试验检测脑组织梗死区中HIF-1α和VEGF两种因子其蛋白的表达结果对照组和实验组中两种蛋白表达量在6h、24h逐渐增高(P0.01),24h时表达量最高,48h、72h、7d时逐渐下降,7d时表达量最低(P0.01)。与对照组相比,实验组各个时间点HIF-1α和VEGF蛋白表达量均升高,具有统计学意义(P0.05)。对HIF-1α、VEGF两种蛋白表达水平进行相关性分析,结果表明VEGF和HIF-1α的表达具有正相关性。结论1 HIF-1α质粒在大鼠脑缺血再灌注损伤中,有助于改善脑组织缺血梗死区,使其缩小。2在脑缺血再灌注损伤中,HIF-1α、VEGF因子在此调控过程中起着非常重要的作用。
[Abstract]:Objective to study the expression and mechanism of HIF-1 伪 and VEGF during cerebral ischemia. Methods 60 adult healthy male SD rats weighing 250 g and 300 g were selected. Rats were randomly divided into two groups: experimental group and control group, 30 rats in each group. Two hours after the establishment of the model, the experimental group was injected with HIF-1 伪 granule solution and the control group was injected with the same amount of PBS buffer. According to the different time after injection, the rats were divided into 6 hours, 24 hours, 48 hours, 72 hours. At 7 days, 6 rats in each subgroup were divided into five time points. The cerebral infarction area of each group was observed by TTC staining to determine the degree of cerebral ischemia, and the protein expression of HIF-1 伪 and VEGF in cerebral infarction area was detected by immunoblotting test. Results 1Compared with the control group, there was no significant difference in the infarct area between the experimental group and the control group at 6 h, 24 h, 48 h (P0.05), but at 72 h and 7 d, there was no significant difference in the infarct area between the experimental group and the control group by TTC staining. The effect of injection of HIF-1 伪 granule solution on the mortality of rats was more significant at the 7th day (P0.01). (2) the death rate of rats injected with HIF-1 伪 granule solution after ischemia reperfusion was the highest among 4h~60h. If the survival time was longer than 72 hours, the mortality was low (P0.01). 3 the expression of HIF-1 伪 and VEGF in cerebral infarction area was detected by immunoblotting assay. The expression of two proteins in the control group and the experimental group was 6 hours. The expression level increased gradually at 24 h (P0.01), the highest at 24 h, the highest at 48 h, at 72 h, gradually decreased at 7 d, and the lowest at 7 d (P 0.01). Compared with the control group, the expression of HIF-1 伪 and VEGF protein in the experimental group was significantly higher than that in the control group (P0.05). The expression levels of HIF-1 伪 and VEGF were analyzed. The results showed that there was a positive correlation between the expression of VEGF 伪 and HIF-1 伪. Conclusion (1) HIF-1 伪 plasmid can improve the ischemic infarct area and reduce it in cerebral ischemia reperfusion injury in rats. 2 in cerebral ischemia reperfusion injury, HIF-1 伪 and VEGF factor play a very important role in the regulation of cerebral ischemia reperfusion injury. [WT5HZ] conclusion\?
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614

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