不同免疫学方法对兔小关节异体移植的影响研究
发布时间：2019-03-09 15:47
【摘要】：目的:本研究采用多种免疫学处理方法,对比研究兔同种异体小关节移植的实验效果,旨在探讨一种减少同种异体小关节移植免疫排斥反应,促进骨折愈合及避免关节退变的有效措施,为临床上行同种异体小关节移植术提供新途径。方法:随机选取48只成年雄性新西兰兔,以新西兰兔上肢第三掌指关节为研究对象,术前1月处死18只新西兰兔取第三掌指关节进行深低温保存,余下30只随机分A、B、C、D、E组,每组实验动物为6只。A组为同种异体关节直接移植组,B组为同种异体关节直接移植+FK560免疫药物组,C组为冷冻处理的同种异体关节移植组,D组为冷冻处理后的同种异体关节移植+FK560免疫药物组,E组为冷冻处理后的同种异体关节移+FK560+MMF+Pred药物组。异体关节移植术后分别于4周将实验各组处死1只新西兰兔观察其大体标本,8周抽取各实验动物组血清进行IL-2及IL-6含量检测,12周进行影像学检查,48周将实验动物全部处死进行常规HE染色切片来观察骨折的愈合以及关节面软骨的情况。对比各组的实验结果,实验数据应用SPSS19.0统计软件分析处理。结果:1)大体结果:术后4周大体标本除A实验组3只新西兰兔创口出现不同程度的红肿、溃烂,其余4组创口均顺利愈合。2)术后8周取兔血清观察白介素-2结果:方差分析显示各组间总差异有统计学意义(F=52.163,P0.001)。B、C组与A组比较P0.001,D、E组与B、C组比较P0.001。B与C组之间无差异(P=0.150),D与E组之间无差异(P=0.721)。结果显示AB、CD、E。术后8周白介素-6结果:方差分析显示各组间总差异有统计学意义(F=482.864,P0.001).B、C组与A组比较P0.001,D、E组与B、C组比较P0.001。但B与C组之间无差异(P=0.164),D与E组之间无差异(P=0.378)。总结果显示AB、CD、E。3)术后12周X线显示:骨折端骨痂生长情况及皮质骨塑形情况,总体相比B、C、D组结果均明显优于A.B组。4)术后48周组织学观察关节软骨面Wakitani score评分:A组得分远高于其它组,B、C组间以及D、E组间差异不大。总体得分为:AB、CD、E。结论:同种异体小关节移植时,供体关节术前经深低温冷冻处理,术后短期使用免疫抑制药抗排斥,不仅可以有效的减少免疫排斥反应,也可以促进自体骨与供体骨更好的愈合,而且能延缓移植关节远期的关节退变,从而最大程度的保存异体移植关节的功能。
[Abstract]:Objective: to compare the experimental results of rabbit facet allograft transplantation with various immunologic methods in order to explore a method to reduce the immune rejection of allogeneic facet transplantation. The effective measures to promote fracture healing and to avoid joint degeneration provide a new way for clinical transplantation of allogeneic facet. Methods: forty-eight adult male New Zealand rabbits were randomly selected. The third metacarpophalangeal joints of the upper limbs of New Zealand rabbits were killed 1 month before operation for cryopreservation. The remaining 30 rabbits were randomly divided into A, B, C, A, B, C. Group D, Group E, 6 animals in each group. Group A: direct transplantation of joints, Group B: direct transplantation of FK560 immunopharmaceuticals, Group C: cryopreserved allografts, Group A: allografts, Group B: allografts, Group C: cryopreserved allografts. Group D was FK560 immunopharmaceutical group after cryopreservation and group E was cryopreserved allogeneic joint transplantation with FK560 MMF Pred drug group. At 4 weeks after allogeneic joint transplantation, 1 New Zealand rabbit was sacrificed to observe the gross specimen, 8 weeks after transplantation, the serum levels of IL-2 and IL-6 were measured, and imaging examination was carried out at 12 weeks. All the animals were killed at 48 weeks and routine HE staining sections were used to observe the healing of fracture and articular cartilage. Comparing the experimental results of each group, the experimental data were analyzed and processed by SPSS19.0 statistical software. Results: 1) Gross results: 4 weeks after operation, except for 3 New Zealand rabbits in experimental group A, there were different degrees of redness and ulceration in the wound. The results of ANOVA showed that there was a significant difference among the four groups (F = 52.163, P 0.001). B, C group compared with A group P 0.001, D, P < 0.01, P < 0.01, There was no difference between P0.001.B and C (P = 0.150), D vs E, P = 0.721). Results show AB,CD,E. Results of IL-6 at 8 weeks after operation: analysis of variance showed that there was a significant difference among the three groups (F = 482.864, P 0.001). B, C group compared with A group P0.001, D, E group compared with B, C group P 0.001. But there was no difference between group B and C (P = 0.164), D vs group E, P = 0.378). The results showed that 12 weeks after AB,CD,E.3, the growth of callus at fracture end and the shape of cortical bone were observed by X-ray, as compared with those of B, C, C, The results of group D were significantly better than those of group A. 4) the Wakitani score scores of articular cartilage surface in group A were significantly higher than those in other groups at 48 weeks after operation: group B, group C and group D, E had little difference. The overall score is: AB,CD,E. Conclusion: during allogeneic facet transplantation, cryopreservation of donor joints before operation and short-term use of immunosuppressive drugs against rejection after transplantation can not only effectively reduce the immune rejection, but also decrease the rejection of the donor joints. It can also promote the healing of autogenous bone and donor bone, and delay the long-term joint degeneration of transplanted joint, thus preserving the function of allogeneic joint to the maximum extent.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R687.4
本文编号：2437615
[Abstract]:Objective: to compare the experimental results of rabbit facet allograft transplantation with various immunologic methods in order to explore a method to reduce the immune rejection of allogeneic facet transplantation. The effective measures to promote fracture healing and to avoid joint degeneration provide a new way for clinical transplantation of allogeneic facet. Methods: forty-eight adult male New Zealand rabbits were randomly selected. The third metacarpophalangeal joints of the upper limbs of New Zealand rabbits were killed 1 month before operation for cryopreservation. The remaining 30 rabbits were randomly divided into A, B, C, A, B, C. Group D, Group E, 6 animals in each group. Group A: direct transplantation of joints, Group B: direct transplantation of FK560 immunopharmaceuticals, Group C: cryopreserved allografts, Group A: allografts, Group B: allografts, Group C: cryopreserved allografts. Group D was FK560 immunopharmaceutical group after cryopreservation and group E was cryopreserved allogeneic joint transplantation with FK560 MMF Pred drug group. At 4 weeks after allogeneic joint transplantation, 1 New Zealand rabbit was sacrificed to observe the gross specimen, 8 weeks after transplantation, the serum levels of IL-2 and IL-6 were measured, and imaging examination was carried out at 12 weeks. All the animals were killed at 48 weeks and routine HE staining sections were used to observe the healing of fracture and articular cartilage. Comparing the experimental results of each group, the experimental data were analyzed and processed by SPSS19.0 statistical software. Results: 1) Gross results: 4 weeks after operation, except for 3 New Zealand rabbits in experimental group A, there were different degrees of redness and ulceration in the wound. The results of ANOVA showed that there was a significant difference among the four groups (F = 52.163, P 0.001). B, C group compared with A group P 0.001, D, P < 0.01, P < 0.01, There was no difference between P0.001.B and C (P = 0.150), D vs E, P = 0.721). Results show AB,CD,E. Results of IL-6 at 8 weeks after operation: analysis of variance showed that there was a significant difference among the three groups (F = 482.864, P 0.001). B, C group compared with A group P0.001, D, E group compared with B, C group P 0.001. But there was no difference between group B and C (P = 0.164), D vs group E, P = 0.378). The results showed that 12 weeks after AB,CD,E.3, the growth of callus at fracture end and the shape of cortical bone were observed by X-ray, as compared with those of B, C, C, The results of group D were significantly better than those of group A. 4) the Wakitani score scores of articular cartilage surface in group A were significantly higher than those in other groups at 48 weeks after operation: group B, group C and group D, E had little difference. The overall score is: AB,CD,E. Conclusion: during allogeneic facet transplantation, cryopreservation of donor joints before operation and short-term use of immunosuppressive drugs against rejection after transplantation can not only effectively reduce the immune rejection, but also decrease the rejection of the donor joints. It can also promote the healing of autogenous bone and donor bone, and delay the long-term joint degeneration of transplanted joint, thus preserving the function of allogeneic joint to the maximum extent.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R687.4
【参考文献】
相关期刊论文 前1条
1 陆军,吴苏稼,施鑫;大块骨关节软骨冷冻保存的实验研究[J];医学研究生学报;2002年03期
，本文编号：2437615
本文链接：https://www.wllwen.com/yixuelunwen/waikelunwen/2437615.html
最近更新
教材专著
