MPPa-PDT抑制骨肉瘤MG-63细胞侵袭和迁移
发布时间：2019-03-11 20:21
【摘要】：目的:探讨焦脱镁叶绿酸-a甲酯介导的光动力疗法(methyl ester pyropheophorbide-a mediated photodynamic therapy,MPPa-PDT)对人骨肉瘤MG-63细胞迁移及侵袭的影响及其可能机制。方法:采用CCK-8检测经MPPa-PDT处理后不同时间点的人骨肉瘤MG-63细胞增殖活性;划痕实验及Transwell实验检测细胞迁移、侵袭能力;Western blotting检测E-钙黏蛋白(E-cad)和基质金属蛋白酶(matrix metalloproteinase,MMP)-2、-9蛋白的表达水平。结果:MPPa-PDT处理MG-63细胞12、24及48 h后MPPa-PDT组细胞增殖能力明显较对照组、MPPa组和LED组细胞降低(均P0.05),且MPPa-PDT组细胞的划痕愈合能力、迁移活力及侵袭能力也较这3组细胞显著下降(均P0.05);MPPa-PDT组细胞E-cad的表达显著高于其他3组,MMP-2、MMP-9的表达则明显低于其他3组(均P0.05)。结论:MPPa-PDT抑制人骨肉瘤MG-63细胞的侵袭和迁移能力;上调E-cad的表达,下调MMP-2、-9的表达可能是其作用机制之一。
[Abstract]:Aim: to investigate the effect of photodynamic therapy (methyl ester pyropheophorbide-a mediated photodynamic therapy,MPPa-PDT) mediated by pyromagnesium chlorophyllin-a methyl ester on migration and invasion of human osteosarcoma MG-63 cells and its possible mechanism. Methods: CCK-8 was used to detect the proliferation activity of human osteosarcoma MG-63 cells treated with MPPa-PDT at different time points, scratch test and Transwell test were used to detect the migration and invasion ability of the cells. The expression levels of E-cadherin (E-cad), matrix metalloproteinases (matrix metalloproteinase,MMP)-2, and protein 9 were detected by Western blotting. Results: the proliferation ability of MG-63 cells treated with MPPa-PDT for 12 h, 24 h and 48 h was significantly lower than that of control group, MPPa group and LED group (P 0.05), and the ability of scratch healing in MPPa-PDT group was significantly lower than that of control group, MPPa group and LED group (P 0.05). The migration activity and invasion ability of the three groups were significantly lower than those of the three groups (P 0.05). The expression of E-cad in MPPa-PDT group was significantly higher than that in the other 3 groups, while the expression of MMP-2,MMP-9 in the other 3 groups was significantly lower than that in the other 3 groups (P 0.05). Conclusion: MPPa-PDT can inhibit the invasion and migration of human osteosarcoma MG-63 cells, up-regulate the expression of E-cad and down-regulate the expression of MMP-2,-9 may be one of its mechanisms.
【作者单位】： 重庆医科大学附属第一医院骨科
【基金】：国家自然科学基金项目(No.81572634) 重庆市教育委员会研究生科研创新项目(No.CYS15141)~~
【分类号】：R738.1
[Abstract]:Aim: to investigate the effect of photodynamic therapy (methyl ester pyropheophorbide-a mediated photodynamic therapy,MPPa-PDT) mediated by pyromagnesium chlorophyllin-a methyl ester on migration and invasion of human osteosarcoma MG-63 cells and its possible mechanism. Methods: CCK-8 was used to detect the proliferation activity of human osteosarcoma MG-63 cells treated with MPPa-PDT at different time points, scratch test and Transwell test were used to detect the migration and invasion ability of the cells. The expression levels of E-cadherin (E-cad), matrix metalloproteinases (matrix metalloproteinase,MMP)-2, and protein 9 were detected by Western blotting. Results: the proliferation ability of MG-63 cells treated with MPPa-PDT for 12 h, 24 h and 48 h was significantly lower than that of control group, MPPa group and LED group (P 0.05), and the ability of scratch healing in MPPa-PDT group was significantly lower than that of control group, MPPa group and LED group (P 0.05). The migration activity and invasion ability of the three groups were significantly lower than those of the three groups (P 0.05). The expression of E-cad in MPPa-PDT group was significantly higher than that in the other 3 groups, while the expression of MMP-2,MMP-9 in the other 3 groups was significantly lower than that in the other 3 groups (P 0.05). Conclusion: MPPa-PDT can inhibit the invasion and migration of human osteosarcoma MG-63 cells, up-regulate the expression of E-cad and down-regulate the expression of MMP-2,-9 may be one of its mechanisms.
【作者单位】： 重庆医科大学附属第一医院骨科
【基金】：国家自然科学基金项目(No.81572634) 重庆市教育委员会研究生科研创新项目(No.CYS15141)~~
【分类号】：R738.1
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