脊髓半横断损伤对大鼠骨折愈合的影响

发布时间：2019-04-01 09:30

【摘要】：背景:研究证明,神经系统显著影响骨折的愈合过程,神经不同层面、不同成分对骨痂影响各不相同。目的:制备脊髓半横断损伤并骨折的大鼠模型,观察神经完整性不同的骨折,愈合时骨痂量的不同和组织形态学变化,探讨其对骨痂生成的影响。方法:雄性SD大鼠48只,分2组,制备单纯骨折模型及脊髓半横断损伤并骨折模型,切断右半侧脊髓。于术后14,28 d分批取材,取胫骨,称量湿质量,X射线观察骨折愈合情况,苏木精-伊红染色观察骨痂组织形态变化,q PCR SYBR GREEN法检测骨折断端骨组织降钙素基因相关肽、骨形态发生蛋白2 m RNA的表达。结果与结论:(1)脊髓半横断损伤并骨折组,对侧胫骨湿质量大于患侧(P0.05),均大于单纯骨折组(P0.05);(2)X射线显示脊髓半横断损伤并骨折,对侧骨痂最大,患侧次之,单纯骨折骨痂最小;(3)苏木精-伊红染色显示,28 d脊髓半横断损伤并骨折组,对侧细胞排列紊乱;患侧纤维骨痂为主,细胞排列较规则;单纯骨折,骨性骨痂为主,细胞排列规则。(4)q PCR SYBR GREEN法结果显示,单纯骨折组降钙素基因相关肽、骨形态发生蛋白2表达较脊髓半横断损伤并骨折组高(P0.05);(5)结果提示,完整神经支配是骨折愈合的必须因素。
[Abstract]:Background: studies have shown that the nervous system significantly affects the healing process of fractures, and that different levels and components of nerve have different effects on callus. Aim: to establish a rat model of spinal cord hemisection injury with fracture, to observe the different fracture of nerve integrity, the different amount of callus and the histomorphological changes during healing, and to explore its effect on the formation of callus. Methods: 48 male SD rats were divided into two groups. Simple fracture model and spinal cord hemisection injury with fracture model were made and right hemisection spinal cord was cut off. On the 14th and 28th day after operation, the tibia was taken in batches to measure wet mass, X-ray was used to observe the healing of fracture, hematoxylin-eosin staining was used to observe the morphological changes of callus tissue, q PCR SYBR GREEN method was used to detect calcitonin gene-related peptide (CGRP) in the broken bone of fracture. Expression of bone morphogenetic protein 2 m RNA. Results & conclusion: (1) in the group of spinal cord hemisection with fracture, the wet mass of the contralateral tibia was greater than that of the affected side (P0.05), which was higher than that of the simple fracture group (P0.05). (2) X-ray showed spinal cord hemisection injury with fracture, the contralateral callus was the largest, the affected side was the second, and the simple fracture callus was the smallest, (3) hematoxylin-eosin staining showed that the contralateral cells were disarranged in the group of spinal cord hemisection injury with fracture 28 days after injury. The fibrous callus in the affected side was dominant, and the arrangement of cells was regular. (4) the expression of calcitonin gene-related peptide (CGRP) and bone morphogenetic protein-2 (BMP-2) in the simple fracture group was higher than that in the spinal cord hemisection injury with fracture group (P0.05). (5) the results suggest that complete innervation is an essential factor in fracture healing.
【作者单位】：厦门大学附属福州第二医院骨科;
【基金】：国家自然科学基金(81302986) 福州市科技计划项目(2013-s-123-12)~~
【分类号】：R683

【相似文献】